Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   A4G13_RS10285 Genome accession   NZ_CP015031
Coordinates   2210116..2210568 (-) Length   150 a.a.
NCBI ID   WP_011199546.1    Uniprot ID   Q65VN9
Organism   Basfia succiniciproducens strain JF4016     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2205116..2215568
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4G13_RS10255 (A4G13_10185) - 2205619..2205891 (-) 273 WP_090654049.1 GNAT family N-acetyltransferase -
  A4G13_RS10260 (A4G13_10190) yacG 2205908..2206126 (-) 219 WP_011199540.1 DNA gyrase inhibitor YacG -
  A4G13_RS10265 (A4G13_10195) coaE 2206104..2206748 (-) 645 WP_090654048.1 dephospho-CoA kinase -
  A4G13_RS10270 (A4G13_10200) - 2206775..2207491 (-) 717 WP_090654046.1 A24 family peptidase -
  A4G13_RS10275 (A4G13_10205) pilC 2207488..2208705 (-) 1218 WP_165898036.1 type II secretion system F family protein Machinery gene
  A4G13_RS10280 (A4G13_10210) pilB 2208702..2210102 (-) 1401 WP_090654044.1 GspE/PulE family protein Machinery gene
  A4G13_RS10285 (A4G13_10215) pilA 2210116..2210568 (-) 453 WP_011199546.1 prepilin peptidase-dependent pilin Machinery gene
  A4G13_RS10290 (A4G13_10220) ampD 2210675..2211244 (+) 570 WP_102030684.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  A4G13_RS10295 (A4G13_10225) - 2211382..2211852 (+) 471 WP_011199548.1 surface-adhesin E family protein -
  A4G13_RS10300 (A4G13_10230) era 2211927..2212880 (-) 954 WP_090654043.1 GTPase Era -
  A4G13_RS10305 (A4G13_10235) rnc 2212877..2213551 (-) 675 WP_090654041.1 ribonuclease III -
  A4G13_RS10310 (A4G13_10240) lepB 2213553..2214275 (-) 723 WP_041639506.1 signal peptidase I -
  A4G13_RS10315 (A4G13_10245) lepB 2214291..2215313 (-) 1023 WP_090654039.1 signal peptidase I -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15845.14 Da        Isoelectric Point: 8.4444

>NTDB_id=176336 A4G13_RS10285 WP_011199546.1 2210116..2210568(-) (pilA) [Basfia succiniciproducens strain JF4016]
MKNYRTLSQYPAIRKGFTLIELMIVIAIIAILATIAIPSYQNYTKRAAISELLQAGAPYKADVELCIYEKGGEKDCSSGA
NGIAEPAKTKGKYVDAVTVSSGTISVTGKGSLSGVSYSTKATGNASEGISWTTTCTPKDIFPAGFCDNKE

Nucleotide


Download         Length: 453 bp        

>NTDB_id=176336 A4G13_RS10285 WP_011199546.1 2210116..2210568(-) (pilA) [Basfia succiniciproducens strain JF4016]
ATGAAAAATTACCGCACTTTATCTCAGTACCCTGCCATTCGCAAAGGTTTTACACTGATTGAATTAATGATTGTTATCGC
TATTATTGCCATTCTCGCTACTATTGCAATTCCCTCTTATCAAAATTACACCAAACGGGCGGCAATTTCCGAATTATTGC
AGGCGGGCGCACCCTATAAAGCCGATGTGGAATTATGTATTTATGAAAAAGGCGGTGAAAAAGACTGCTCAAGCGGTGCC
AACGGTATTGCCGAACCGGCGAAAACAAAAGGGAAATACGTTGATGCCGTCACCGTGAGCAGTGGTACGATTTCCGTTAC
CGGTAAAGGCAGTTTATCCGGTGTCAGTTATTCGACCAAAGCTACCGGTAATGCTTCGGAAGGTATCAGTTGGACGACAA
CCTGCACGCCTAAAGATATTTTCCCGGCAGGTTTTTGTGATAATAAAGAATAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(14-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q65VN9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

61.268

94.667

0.58
  pilA Haemophilus influenzae Rd KW20

60.563

94.667

0.573
  pilA Glaesserella parasuis strain SC1401

52.258

100

0.54
  pilA Acinetobacter nosocomialis M2

41.429

93.333

0.387

Multiple sequence alignment