Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   A4G13_RS10280 Genome accession   NZ_CP015031
Coordinates   2208702..2210102 (-) Length   466 a.a.
NCBI ID   WP_090654044.1    Uniprot ID   -
Organism   Basfia succiniciproducens strain JF4016     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2203702..2215102
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4G13_RS10250 (A4G13_10180) - 2203818..2205209 (-) 1392 WP_011199535.1 sodium:alanine symporter family protein -
  A4G13_RS10255 (A4G13_10185) - 2205619..2205891 (-) 273 WP_090654049.1 GNAT family N-acetyltransferase -
  A4G13_RS10260 (A4G13_10190) yacG 2205908..2206126 (-) 219 WP_011199540.1 DNA gyrase inhibitor YacG -
  A4G13_RS10265 (A4G13_10195) coaE 2206104..2206748 (-) 645 WP_090654048.1 dephospho-CoA kinase -
  A4G13_RS10270 (A4G13_10200) - 2206775..2207491 (-) 717 WP_090654046.1 A24 family peptidase -
  A4G13_RS10275 (A4G13_10205) pilC 2207488..2208705 (-) 1218 WP_165898036.1 type II secretion system F family protein Machinery gene
  A4G13_RS10280 (A4G13_10210) pilB 2208702..2210102 (-) 1401 WP_090654044.1 GspE/PulE family protein Machinery gene
  A4G13_RS10285 (A4G13_10215) pilA 2210116..2210568 (-) 453 WP_011199546.1 prepilin peptidase-dependent pilin Machinery gene
  A4G13_RS10290 (A4G13_10220) ampD 2210675..2211244 (+) 570 WP_102030684.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  A4G13_RS10295 (A4G13_10225) - 2211382..2211852 (+) 471 WP_011199548.1 surface-adhesin E family protein -
  A4G13_RS10300 (A4G13_10230) era 2211927..2212880 (-) 954 WP_090654043.1 GTPase Era -
  A4G13_RS10305 (A4G13_10235) rnc 2212877..2213551 (-) 675 WP_090654041.1 ribonuclease III -
  A4G13_RS10310 (A4G13_10240) lepB 2213553..2214275 (-) 723 WP_041639506.1 signal peptidase I -

Sequence

Protein


Download         Length: 466 a.a.        Molecular weight: 53286.01 Da        Isoelectric Point: 6.9611

>NTDB_id=176335 A4G13_RS10280 WP_090654044.1 2208702..2210102(-) (pilB) [Basfia succiniciproducens strain JF4016]
MQSIKIIAGNGEQYMIDHELWQRNQQQQHVLLRYLAVPIKEEEQKLWLAIDNVENIAACETFSFLTGKIVEPVLVSNETL
KSLLQPDEPQSLSIEETSLIYTESLSQNKENKNTDEPIIRLLNNIFESALSKNASDIHFEPQKNQLRIRFRIDGVLQQQT
PVNLSLAGRIISRLKLLAKLDISETRLPQDGRFDFKTTFAETLDFRISTLATRNGEKIVLRLQQNKPVDFSFEQLGMEPA
QQQKLEQALNQPQGLILVTGPTGSGKSITLYTALQWLNSASKHIMTAEDPIEIELEGIIQTQIQPQIGLSFSRLLRTFLR
QDPDVIMLGEIRDDESAQMALRAAQTGHLVLSTLHTNDAYSAISRLLQLGIKQHEIDNSLLLIIAQRLVRKRCQKCGQFS
KSFINCDCHQGYRGRIGIYQFLHPWWQAQKWQYVTDFPSLYQAAKNKVQQQITDKQELLRVLGSEK

Nucleotide


Download         Length: 1401 bp        

>NTDB_id=176335 A4G13_RS10280 WP_090654044.1 2208702..2210102(-) (pilB) [Basfia succiniciproducens strain JF4016]
ATGCAATCAATTAAAATCATTGCCGGCAATGGCGAACAATACATGATTGATCACGAATTGTGGCAACGCAATCAACAGCA
GCAACATGTATTGTTACGCTATCTTGCCGTACCCATCAAAGAGGAAGAGCAAAAACTATGGCTTGCCATTGATAATGTAG
AAAATATCGCCGCTTGCGAAACCTTTTCCTTTTTAACCGGCAAAATTGTTGAACCCGTATTAGTCAGTAACGAAACCTTA
AAATCATTATTGCAACCGGATGAACCGCAATCATTAAGTATTGAAGAAACATCATTGATTTATACGGAAAGCCTCTCTCA
AAATAAGGAAAATAAAAATACCGACGAACCTATTATCCGTTTATTAAATAATATTTTTGAGAGCGCCCTAAGTAAAAATG
CCTCCGATATTCATTTCGAGCCGCAAAAAAACCAGCTTCGAATTCGTTTCAGAATCGACGGTGTGTTGCAACAACAAACA
CCCGTTAATCTTTCTTTAGCAGGGCGCATTATTTCAAGATTAAAACTGTTGGCAAAATTAGATATCAGCGAAACCCGCTT
ACCGCAAGACGGACGTTTTGATTTCAAAACCACCTTCGCCGAAACCCTGGATTTTCGTATATCGACCCTTGCCACCAGAA
ACGGTGAAAAAATCGTGCTGCGTTTACAGCAAAATAAACCGGTGGATTTCAGCTTTGAACAACTTGGTATGGAGCCTGCG
CAACAACAAAAACTCGAACAAGCACTTAATCAGCCGCAAGGGTTAATTTTAGTGACCGGCCCGACCGGTAGCGGGAAAAG
TATCACCCTTTATACGGCGCTGCAATGGCTTAATAGCGCCTCAAAACATATAATGACGGCGGAAGATCCCATTGAAATTG
AACTTGAAGGGATTATTCAAACCCAAATACAGCCTCAAATAGGCTTGAGTTTCAGCCGACTGCTGCGCACATTTCTACGT
CAGGATCCGGATGTCATTATGCTAGGTGAAATCCGTGATGATGAAAGTGCTCAAATGGCATTACGTGCAGCGCAAACCGG
GCATTTGGTTCTATCCACCTTGCATACTAACGATGCTTACTCCGCAATTTCACGCTTATTGCAACTGGGAATTAAACAGC
ATGAAATCGATAATAGCCTGCTCCTTATTATCGCCCAACGCTTAGTGCGAAAGCGTTGCCAAAAGTGCGGTCAATTTTCC
AAAAGTTTTATCAACTGCGACTGTCATCAAGGTTACCGAGGACGTATCGGCATTTATCAGTTTCTTCATCCCTGGTGGCA
AGCTCAGAAATGGCAATATGTTACGGATTTTCCTTCCCTTTATCAGGCGGCAAAAAACAAAGTACAACAACAAATTACCG
ATAAGCAGGAATTATTGCGCGTATTAGGAAGTGAAAAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(124-391)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(12-85)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

66.304

98.712

0.655
  pilB Haemophilus influenzae Rd KW20

65.795

98.498

0.648
  pilB Glaesserella parasuis strain SC1401

60.222

96.567

0.582
  pilB Vibrio parahaemolyticus RIMD 2210633

41.286

100

0.427
  pilB Vibrio campbellii strain DS40M4

41.164

100

0.425
  pilB Vibrio cholerae strain A1552

40.286

100

0.423
  pilB Legionella pneumophila strain ERS1305867

38.956

100

0.416
  pilB Acinetobacter baylyi ADP1

37.475

100

0.401
  pilB Acinetobacter baumannii D1279779

37.63

100

0.388

Multiple sequence alignment