Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   AYO71_RS04800 Genome accession   NZ_CP014947
Coordinates   1075052..1077130 (+) Length   692 a.a.
NCBI ID   WP_041477572.1    Uniprot ID   -
Organism   Pseudomonas koreensis strain D26     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1070052..1082130
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AYO71_RS04780 (AYO71_04780) comM 1072267..1073331 (+) 1065 WP_041477568.1 pilus assembly protein PilM Machinery gene
  AYO71_RS04785 (AYO71_04785) - 1073331..1073897 (+) 567 WP_041477569.1 PilN domain-containing protein -
  AYO71_RS04790 (AYO71_04790) pilO 1073894..1074517 (+) 624 WP_041477570.1 type 4a pilus biogenesis protein PilO -
  AYO71_RS04795 (AYO71_04795) - 1074514..1075038 (+) 525 WP_041477571.1 pilus assembly protein PilP -
  AYO71_RS04800 (AYO71_04800) pilQ 1075052..1077130 (+) 2079 WP_041477572.1 type IV pilus secretin PilQ Machinery gene
  AYO71_RS04805 (AYO71_04805) aroK 1077135..1077653 (+) 519 WP_024011270.1 shikimate kinase AroK -
  AYO71_RS04810 (AYO71_04810) aroB 1077806..1078906 (+) 1101 WP_041477573.1 3-dehydroquinate synthase -
  AYO71_RS04815 (AYO71_04815) - 1078917..1080503 (+) 1587 WP_041477574.1 AAA family ATPase -

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 74836.09 Da        Isoelectric Point: 5.4106

>NTDB_id=175286 AYO71_RS04800 WP_041477572.1 1075052..1077130(+) (pilQ) [Pseudomonas koreensis strain D26]
MNRIFSTLGFSLWIALVSPMVMAASLKALDVAALPGDRVELKLSFDGPPPQPKGYTTESPARIALDLPGVTSQLANKNLD
LGSGNARTATVAEAKDRTRLIVSLTQLAPYSTRVEGNSLFVVVGQGASTTAPRPAAVAPRAATAAAAPARPAAQRSRAIR
GVDFQRGTEGEGNVVIDLSDPTIAPDIQEHDGKIILSFARTQLPEKLRVRLDVKDFATPVQFVNAAVTGDRAVISVEPSG
TFDYSTFQTDNKLTVSVRPMSIDDLQKRNADRQAYVGDKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQN
VPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEA
KVDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVQNKGNWNASGVN
GSSSTIGTPGSTGTNSPFVDMGTVNNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPY
QEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSN
TQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=175286 AYO71_RS04800 WP_041477572.1 1075052..1077130(+) (pilQ) [Pseudomonas koreensis strain D26]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGGTGTCACCGATGGTAATGGCGGCCAGTCTGAA
AGCGCTGGATGTTGCCGCGCTGCCGGGTGACCGCGTCGAACTGAAGCTGTCGTTCGACGGCCCGCCACCACAGCCCAAGG
GCTACACAACCGAGTCACCGGCACGCATTGCACTGGATCTGCCGGGCGTGACCAGCCAGTTGGCCAACAAGAATCTTGAT
CTGGGCAGCGGCAACGCGCGCACGGCCACGGTGGCCGAAGCCAAGGATCGCACGCGGCTGATTGTCAGCCTGACGCAGTT
GGCGCCTTACAGCACGCGGGTCGAGGGCAATAGCCTGTTTGTGGTGGTCGGTCAGGGCGCCTCCACGACAGCGCCGCGTC
CGGCTGCCGTTGCACCGCGTGCCGCCACGGCCGCTGCGGCACCGGCCAGACCAGCCGCGCAACGCAGCAGGGCGATTCGC
GGTGTCGATTTCCAGCGCGGCACCGAGGGCGAGGGCAACGTAGTCATCGACCTCTCTGATCCGACCATCGCCCCGGATAT
CCAGGAGCACGATGGCAAGATCATCCTCAGTTTCGCCCGTACGCAACTGCCGGAAAAACTGCGCGTGCGCCTCGACGTCA
AGGACTTCGCCACGCCGGTGCAGTTCGTCAATGCCGCGGTAACCGGTGATCGCGCAGTGATCAGCGTCGAGCCCAGCGGC
ACCTTCGACTACTCCACGTTTCAGACCGACAACAAGCTCACCGTGAGCGTTCGGCCGATGAGCATCGACGACCTGCAAAA
GCGCAACGCCGACCGTCAGGCCTATGTCGGCGACAAGCTCTCGCTGAATTTTCAGGACATCGATGTGCGCTCGGTGCTGC
AACTGATCGCGGACTTCACCAATCTCAACCTGGTGGCGAGCGACACGGTGCAGGGCGGTATCACCTTGCGCCTGCAAAAC
GTGCCGTGGGATCAGGCACTGGATCTGGTGTTGAAAACCAAGGGGCTGGACAAGCGCAAGATCGGCAACGTGCTGCTGGT
CGCGCCGGCCGATGAAATTGCCGCCCGCGAGCGTCAGGAACTGGAATCGCAGAAGCAGATCGCCGAGCTGGCGCCCCTGC
GTCGTGAGCTGCTGCAAGTCAATTACGCCAAGGCAGCGGACATTGCCAAGCTGTTCCAGTCGGTGACCAGTGCCGAGGCC
AAGGTCGACGAGCGCGGTTCGATTACCGTCGATGAGCGAACCAACAACATCATTGCCTACCAGACCCAGGATCGCCTCGA
CGAACTCCGGCGGATCGTCGCGCAGCTGGATATTCCGGTACGCCAGGTGATGATCGAGGCGCGCATCGTTGAGGCCAACG
TCGATTATGACAAGAGCCTGGGCGTGCGCTGGGGCGGTTCGGTGCAGAACAAGGGCAACTGGAACGCGTCCGGGGTCAAT
GGCTCCTCGAGCACCATCGGTACGCCGGGCAGCACCGGCACCAACTCGCCGTTCGTCGACATGGGCACGGTCAACAATAC
CTCGGGGATCGGCATCGCGTTCATCACCGACAACGTGCTGCTGGATCTGGAACTGACGGCGATGGAAAAGACCGGCAACG
GTGAAATCGTCTCGCAGCCGAAGGTGGTCACCTCGGACAAGGAAACCGCAAAAATCCTCAAAGGCACCGAGATTCCCTAT
CAGGAGGCCAGCTCCAGCGGCGCGACCTCGGTGTCGTTCAAGGAGGCTTCGCTGTCACTGGAAGTCACCCCGCAGATCAC
CCCGGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCCGATTACCTGAACAAAGTGCAGGATGTGCCGC
CGATCAAGAAAAACGAGGTCAACGCCAAAGTGCTGGTAAATGACGGCGAGACCATCGTAATCGGCGGTGTTTTTTCAAAT
ACTCAGAGCAAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGT
TTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(303-349)

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)

AMIN domain (AMIN)

(37-122)

Bacterial type II and III secretion system protein (Secretin)

(527-683)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.067

100

0.746
  comQ Acinetobacter baylyi ADP1

41.787

100

0.419
  pilQ Acinetobacter baumannii strain A118

37.652

100

0.403
  pilQ Acinetobacter baumannii D1279779

37.652

100

0.403
  pilQ Legionella pneumophila strain ERS1305867

38.214

98.699

0.377
  pilQ Legionella pneumophila strain Lp02

38.123

98.555

0.376

Multiple sequence alignment