Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PMA3_RS01505 Genome accession   NZ_CP014870
Coordinates   328428..330509 (+) Length   693 a.a.
NCBI ID   WP_064675509.1    Uniprot ID   A0A191YLZ0
Organism   Pseudomonas silesiensis strain A3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 323428..335509
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PMA3_RS01485 (PMA3_01485) comM 325643..326707 (+) 1065 WP_064675505.1 pilus assembly protein PilM Machinery gene
  PMA3_RS01490 (PMA3_01490) - 326707..327270 (+) 564 WP_064675506.1 PilN domain-containing protein -
  PMA3_RS01495 (PMA3_01495) pilO 327267..327890 (+) 624 WP_064675507.1 type 4a pilus biogenesis protein PilO -
  PMA3_RS01500 (PMA3_01500) - 327887..328414 (+) 528 WP_064675508.1 pilus assembly protein PilP -
  PMA3_RS01505 (PMA3_01505) pilQ 328428..330509 (+) 2082 WP_064675509.1 type IV pilus secretin PilQ Machinery gene
  PMA3_RS01510 (PMA3_01510) aroK 330514..331032 (+) 519 WP_010464421.1 shikimate kinase AroK -
  PMA3_RS01515 (PMA3_01515) aroB 331183..332283 (+) 1101 WP_064675510.1 3-dehydroquinate synthase -
  PMA3_RS01520 (PMA3_01520) - 332293..333882 (+) 1590 WP_064675511.1 AAA family ATPase -

Sequence

Protein


Download         Length: 693 a.a.        Molecular weight: 75044.45 Da        Isoelectric Point: 5.8358

>NTDB_id=174903 PMA3_RS01505 WP_064675509.1 328428..330509(+) (pilQ) [Pseudomonas silesiensis strain A3]
MNRIFSTLGISLWIALLSPMVLAANLKTLDVAALPGDRIELKLSFDGPPPTPRGYTTESPARIALDLPGVVSQLKSKNRE
LGGGNARSATVVEAKDRTRLIINLTQLTPYDARVEGNTLFVVVGQGATKAAKAAPRPASAPRAATATPAPARTYAPVGKA
IRGVDFQRGTQGEGNVVIDLSDPSIAPDIQERDGKIILGFARTQLPEPLRVRLDVKDFATPVQFVNASATGDRATISIEP
TGAFDYSTYQTDNKLTVSIRPMTVDDLQKRNAERFAYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRL
QNVPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSA
EAKIDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVQKGNWNGSGV
NGSSTTIGTPGSTSTNSPFVDLGTAGNTSGLGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIP
YQEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFS
NTQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSH

Nucleotide


Download         Length: 2082 bp        

>NTDB_id=174903 PMA3_RS01505 WP_064675509.1 328428..330509(+) (pilQ) [Pseudomonas silesiensis strain A3]
ATGAACAGGATTTTCTCCACCCTCGGTATTTCGCTATGGATAGCGCTGCTGTCGCCGATGGTACTAGCGGCCAACCTCAA
GACGCTGGATGTCGCGGCGTTACCAGGAGATCGCATCGAGTTGAAGTTGTCTTTCGATGGACCGCCCCCGACACCCCGTG
GTTACACGACGGAGTCGCCGGCACGGATTGCACTGGACCTGCCCGGGGTGGTCAGTCAATTGAAGAGCAAGAATCGTGAA
CTGGGGGGCGGCAATGCCCGCAGCGCCACGGTAGTGGAGGCGAAGGATCGCACGCGGCTGATCATCAACCTTACCCAGTT
GACCCCGTATGACGCGCGGGTCGAAGGCAACACGCTGTTCGTGGTGGTCGGGCAAGGCGCCACGAAGGCCGCGAAGGCCG
CGCCCCGGCCCGCCAGTGCGCCTCGCGCTGCCACTGCAACGCCAGCCCCGGCCAGGACTTATGCGCCGGTTGGCAAAGCC
ATCCGGGGCGTGGATTTCCAGCGCGGCACCCAGGGCGAAGGCAATGTGGTCATCGATCTGTCGGACCCCTCCATCGCCCC
GGACATTCAGGAGCGTGACGGCAAGATCATCCTCGGTTTCGCCAGGACACAACTGCCCGAGCCACTGCGGGTGCGCCTGG
ACGTCAAGGACTTCGCCACGCCCGTGCAGTTCGTCAATGCCAGCGCCACGGGCGATCGGGCCACCATCAGCATCGAGCCC
ACCGGTGCTTTTGACTATTCGACCTATCAGACCGACAACAAGCTGACGGTCAGCATCCGGCCGATGACCGTCGACGACCT
GCAAAAACGCAACGCCGAGCGTTTCGCCTACACCGGTGAAAAGCTCTCGCTGAATTTCCAGGACATCGATGTGCGCTCGG
TGCTGCAACTGATCGCCGATTTCACCAACCTCAACCTGGTGGCCAGCGACACGGTGCAGGGCGGCATCACCCTGCGATTG
CAGAACGTGCCGTGGGATCAGGCGCTGGACCTGGTGCTGAAAACCAAAGGCCTGGATAAACGCAAGATCGGCAACGTGTT
GCTGGTGGCGCCCGCCGATGAAATCGCCGCGCGCGAACGCCAGGAGCTCGAGTCGCAAAAGCAGATCGCCGAACTGGCGC
CTTTGCGTCGCGAGCTGCTGCAGGTCAACTATGCCAAGGCCGCGGATATCGCCAAGCTGTTCCAGTCGGTGACCAGTGCA
GAGGCAAAAATCGACGAACGGGGCTCGATCACCGTCGATGAGCGGACCAACAACATCATTGCCTACCAGACCCAGGACCG
CCTTGATGAACTTCGTCGTATCGTTGCGCAGCTGGATATTCCGGTGCGACAGGTGATGATCGAGGCGCGCATCGTCGAGG
CCAATGTCGATTACGACAAAAGCCTCGGTGTGCGCTGGGGCGGTTCGGTGCAGAAAGGTAACTGGAACGGCTCCGGGGTC
AACGGCTCCTCGACCACCATTGGTACGCCGGGCAGTACCAGCACCAACTCGCCGTTCGTCGACTTGGGCACCGCCGGCAA
CACCTCGGGACTGGGTATCGCGTTCATCACCGACAATGTGTTGCTGGACCTGGAGCTGACCGCGATGGAGAAGACCGGCA
ACGGCGAAATCGTCTCGCAGCCCAAGGTGGTCACGTCCGACAAGGAGACCGCGAAAATCCTCAAGGGCACTGAAATTCCC
TATCAGGAAGCCAGTTCCAGCGGCGCCACGTCGGTATCGTTCAAGGAGGCCTCGCTGTCGCTGGAAGTGACGCCGCAAAT
CACCCCGGACAACCGGATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGATTACTTGAACAAGGTCCAGGATGTGC
CGCCGATCAAGAAAAACGAGGTCAATGCCAAGGTGCTGGTCAATGACGGCGAGACCATCGTGATCGGCGGGGTTTTCTCA
AATACTCAGAGTAAGGTTGTAGATAAGGTGCCATTTCTCGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGACGT
GGTTTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGAGTCATT
GA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(528-684)

AMIN domain (AMIN)

(37-122)

Bacterial type II/III secretion system short domain (Secretin_N)

(379-444)

Secretin and TonB N terminus short domain (STN)

(305-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A191YLZ0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.95

100

0.762
  comQ Acinetobacter baylyi ADP1

41.583

100

0.417
  pilQ Acinetobacter baumannii strain A118

37.766

100

0.41
  pilQ Acinetobacter baumannii D1279779

37.766

100

0.41
  pilQ Legionella pneumophila strain Lp02

37.408

97.98

0.367
  pilQ Legionella pneumophila strain ERS1305867

37.278

97.547

0.364

Multiple sequence alignment