Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   PMA3_RS01485 Genome accession   NZ_CP014870
Coordinates   325643..326707 (+) Length   354 a.a.
NCBI ID   WP_064675505.1    Uniprot ID   A0A191YM94
Organism   Pseudomonas silesiensis strain A3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 320643..331707
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PMA3_RS01475 (PMA3_01475) - 321854..322801 (+) 948 WP_064680598.1 IS110 family transposase -
  PMA3_RS01480 (PMA3_01480) - 322984..325428 (-) 2445 WP_420848678.1 penicillin-binding protein 1A -
  PMA3_RS01485 (PMA3_01485) comM 325643..326707 (+) 1065 WP_064675505.1 pilus assembly protein PilM Machinery gene
  PMA3_RS01490 (PMA3_01490) - 326707..327270 (+) 564 WP_064675506.1 PilN domain-containing protein -
  PMA3_RS01495 (PMA3_01495) pilO 327267..327890 (+) 624 WP_064675507.1 type 4a pilus biogenesis protein PilO -
  PMA3_RS01500 (PMA3_01500) - 327887..328414 (+) 528 WP_064675508.1 pilus assembly protein PilP -
  PMA3_RS01505 (PMA3_01505) pilQ 328428..330509 (+) 2082 WP_064675509.1 type IV pilus secretin PilQ Machinery gene
  PMA3_RS01510 (PMA3_01510) aroK 330514..331032 (+) 519 WP_010464421.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38197.61 Da        Isoelectric Point: 4.9112

>NTDB_id=174902 PMA3_RS01485 WP_064675505.1 325643..326707(+) (comM) [Pseudomonas silesiensis strain A3]
MLGLFNKKANTLLGIDISSTSVKLLELSRQGDRYRVEAYAVEPLPSSAVVEKNIAELEGVGQALSRVLIKAKTSLRNVAV
AVAGSAVITKTIEMDAGLSDDEMENQLKIEADQYIPYPLDEVAIDFEVQGVSARNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSYGLLTRHLAASQEGLTVAVVDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLTV
EQAGLAKKQGGLPDDYVSEVLQPFREALVQQVSRSLQFFFASGQYNSVDHILLAGGTASVSGLDRLIEQRLSTPTRVANP
FSEMSLSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=174902 PMA3_RS01485 WP_064675505.1 325643..326707(+) (comM) [Pseudomonas silesiensis strain A3]
GTGTTAGGACTCTTCAATAAAAAGGCCAATACGCTGCTGGGGATCGACATCAGTTCCACGTCGGTAAAGCTTCTGGAACT
CAGCCGCCAGGGTGATCGCTACCGGGTCGAGGCCTATGCGGTCGAGCCACTGCCCTCCAGCGCGGTGGTCGAGAAAAATA
TCGCCGAGCTCGAAGGCGTGGGTCAGGCCCTTTCACGCGTGTTGATCAAGGCCAAAACCAGCCTCAGGAACGTCGCCGTA
GCCGTGGCCGGTTCGGCAGTGATCACCAAGACCATCGAGATGGATGCCGGCCTTTCCGACGACGAGATGGAAAACCAGCT
GAAGATCGAGGCCGATCAATACATCCCTTATCCATTGGACGAAGTCGCCATCGACTTTGAAGTCCAGGGCGTGTCGGCGC
GCAACCCCGAGCGGGTCAATGTGCTGCTGGCGGCCTGTCGCAAGGAGAACGTCGAAGTCCGTGAAGCGGCCTTGGCCCTG
GCCGGCCTGACAGCTCGGGTAGTCGACGTCGAGGCCTATGCGCTGGAGCGATCGTACGGCCTGCTGACGCGTCATCTGGC
GGCCTCCCAGGAGGGGCTGACGGTGGCGGTGGTCGACATCGGCGCCACCATGACCACCCTGAGCGTGCTGCACAACGGGC
GCATCATCTATACCCGCGAGCAATTGTTCGGCGGTCGCCAGCTGACGGAAGAAATCCAGCGCCGTTACGGGCTGACTGTT
GAACAGGCAGGGTTGGCGAAGAAACAGGGCGGTCTGCCGGACGATTACGTCAGCGAGGTCCTGCAGCCGTTTCGCGAGGC
GCTGGTGCAGCAGGTGTCCCGTTCATTGCAGTTTTTCTTCGCCTCCGGCCAGTACAACTCGGTCGATCACATCCTGCTGG
CCGGCGGTACCGCTTCGGTCTCGGGGCTGGACCGCTTGATCGAGCAGCGACTGAGCACCCCCACGCGAGTGGCCAATCCC
TTCTCCGAAATGAGCCTGAGCAGCAAGGTCAACGCCGGTGCCCTGGCCAGTGATGCCCCGGCCTTGATGATTGCCTGCGG
GCTGGCCCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A191YM94

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

55.65

100

0.557
  pilM Acinetobacter baumannii D1279779

55.367

100

0.554
  comM Acinetobacter baylyi ADP1

53.955

100

0.54
  pilM Legionella pneumophila strain ERS1305867

48.305

100

0.483

Multiple sequence alignment