Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   A3218_RS10510 Genome accession   NZ_CP014867
Coordinates   2437194..2439266 (-) Length   690 a.a.
NCBI ID   WP_062823903.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis strain 189 isolate 189     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2432194..2444266
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A3218_RS10495 (A3218_10495) - 2433822..2435411 (-) 1590 WP_062823901.1 AAA family ATPase -
  A3218_RS10500 (A3218_10500) aroB 2435422..2436522 (-) 1101 WP_062823902.1 3-dehydroquinate synthase -
  A3218_RS10505 (A3218_10505) aroK 2436671..2437189 (-) 519 WP_009041824.1 shikimate kinase AroK -
  A3218_RS10510 (A3218_10510) pilQ 2437194..2439266 (-) 2073 WP_062823903.1 type IV pilus secretin PilQ Machinery gene
  A3218_RS10515 (A3218_10515) - 2439280..2439807 (-) 528 WP_062823904.1 pilus assembly protein PilP -
  A3218_RS10520 (A3218_10520) pilO 2439804..2440421 (-) 618 WP_009041821.1 type 4a pilus biogenesis protein PilO -
  A3218_RS10525 (A3218_10525) - 2440418..2440984 (-) 567 WP_009041820.1 PilN domain-containing protein -
  A3218_RS10530 (A3218_10530) pilM 2440984..2442036 (-) 1053 WP_062823905.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 690 a.a.        Molecular weight: 74712.87 Da        Isoelectric Point: 5.2124

>NTDB_id=174876 A3218_RS10510 WP_062823903.1 2437194..2439266(-) (pilQ) [Pseudomonas chlororaphis strain 189 isolate 189]
MNRIFSAFGIALWMALLSPMAMAASLKALDVAALPGDRLELKLTFDGPVPEPRGYTTDQPARIALDLPGVVSQLADKSRD
LGNGNARSVTVVESGQRTRVVVNLVALAPYSTRVAGNQLFVVIGQGATAALSAPGQPAVASGRAKAVASVGRSIRSVDFQ
RGEQGEGNVLIDLSDSEMSPDIQERDGKLVLTFAKARLPEPLRVRLDVKDFATPVQFVSARAESGKAIVTIEPGGTFDYS
TYQTDNKLTVSVRPMTSGDLQKRNAERSAYNGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPWDQ
ALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLEPLRRELLQVNYAKAAEIAKLFQSVTRAEDKSDER
GSITVDERTNNIIAYQTRERLDELRRIVSQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNQGNWNASGVSNGANG
SSTIGTPGSTSSNSPFVDLGTTANTSGLGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQE
AASSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLINDGETIVIGGVFSNTQ
SKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2073 bp        

>NTDB_id=174876 A3218_RS10510 WP_062823903.1 2437194..2439266(-) (pilQ) [Pseudomonas chlororaphis strain 189 isolate 189]
ATGAACAGGATCTTTTCAGCCTTCGGTATTGCGCTATGGATGGCGTTGCTTTCACCGATGGCCATGGCGGCCAGCCTCAA
GGCCCTGGATGTCGCGGCACTGCCGGGGGATCGCCTGGAGTTGAAGCTGACCTTCGATGGACCGGTGCCCGAGCCGCGCG
GCTATACCACCGACCAGCCGGCGCGGATCGCCCTCGATCTGCCCGGTGTCGTCAGCCAGCTGGCAGACAAGAGCCGTGAC
CTGGGCAATGGCAATGCGCGCAGTGTGACGGTGGTCGAATCGGGGCAGCGTACCCGGGTTGTCGTCAATCTGGTCGCGCT
GGCGCCCTACAGCACTCGGGTCGCTGGCAATCAGCTGTTTGTGGTGATCGGCCAGGGGGCGACGGCGGCGCTGAGTGCGC
CGGGGCAGCCGGCTGTCGCGTCGGGGCGGGCCAAGGCCGTGGCTTCCGTGGGTCGGTCGATTCGCAGCGTGGATTTCCAG
CGCGGCGAGCAGGGCGAGGGTAATGTACTGATCGACCTCTCCGACTCGGAAATGAGCCCGGACATACAGGAGCGCGACGG
CAAACTCGTCCTCACCTTCGCCAAGGCCCGCCTGCCCGAGCCCCTGCGGGTCAGGCTGGACGTCAAGGATTTCGCCACCC
CCGTGCAGTTCGTCAGCGCCCGGGCGGAGTCCGGCAAGGCCATCGTCACCATCGAGCCCGGCGGCACTTTCGATTACTCG
ACCTACCAGACGGATAACAAACTCACGGTGAGCGTCAGGCCGATGACCTCTGGCGACCTGCAGAAACGCAACGCCGAGCG
CTCGGCCTACAACGGCGAAAAGCTCTCGCTGAACTTCCAGGACATCGAGGTTCGCTCGGTGCTGCAACTGATCGCCGACT
TCACCAACCTCAACCTGGTGGCCAGCGATACGGTGCAGGGCGGCATTACCTTGCGCCTGCAGAATGTGCCGTGGGACCAG
GCCCTCGATCTGGTGCTCAAGACCAAGGGGCTGGACAAGCGCAAGGTGGGCAACGTGTTGCTGGTGGCGCCGGCCGATGA
AATCGCTGCCCGCGAACGCCAGGAATTGGAGTCGCAGAAGCAGATCGCCGACCTGGAACCATTGCGGCGCGAACTGCTGC
AGGTCAACTACGCCAAGGCCGCGGAGATCGCCAAGTTGTTCCAGTCGGTCACCCGCGCTGAGGACAAGTCCGACGAACGC
GGGTCGATCACTGTCGATGAGCGGACCAACAACATCATCGCCTACCAGACCCGGGAGCGCCTGGACGAGCTGCGGCGGAT
CGTCAGCCAGCTGGATATCCCGGTGCGCCAGGTGATGATCGAGGCGCGGATCGTCGAGGCCAACGTCGACTACGACAAAA
GCCTGGGCGTGCGCTGGGGCGGTTCGATCCAGAACCAGGGCAACTGGAATGCCTCGGGGGTCAGCAACGGCGCCAATGGT
TCCTCCACCATAGGCACGCCGGGTAGTACCAGCAGCAACTCACCGTTCGTCGACCTGGGCACTACCGCCAATACCTCCGG
CCTGGGTATCGCCTTCATCACCGACAATGTGTTGCTGGATCTTGAGCTGACAGCCATGGAAAAGACCGGTAACGGGGAAA
TCGTCTCCCAGCCCAAGGTCGTCACGTCCGACAAGGAGACGGCGAAGATCCTCAAGGGCACGGAAATCCCCTATCAGGAA
GCCGCCTCCAGCGGGGCGACCTCGGTGTCGTTCAAGGAGGCCTCGCTGTCCCTGGAGGTGACGCCACAGATCACCCCGGA
TAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAGCCGGACTACCTGAACAAAGTCCAGGACGTGCCGCCGATCA
AGAAGAACGAGGTCAACGCCAAGGTCCTGATCAACGATGGAGAGACCATCGTCATCGGTGGAGTTTTCTCCAATACGCAA
AGCAAGGTTGTAGATAAGGTGCCATTTCTCGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGACGTGGTGTCGGA
GAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(372-437)

Bacterial type II and III secretion system protein (Secretin)

(525-681)

AMIN domain (AMIN)

(40-122)

Secretin and TonB N terminus short domain (STN)

(298-344)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

70.769

100

0.733
  comQ Acinetobacter baylyi ADP1

40.055

100

0.423
  pilQ Acinetobacter baumannii strain A118

37.82

100

0.407
  pilQ Acinetobacter baumannii D1279779

37.82

100

0.407
  pilQ Legionella pneumophila strain ERS1305867

38.187

97.536

0.372
  pilQ Legionella pneumophila strain Lp02

38.187

97.536

0.372

Multiple sequence alignment