Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   A3218_RS10530 Genome accession   NZ_CP014867
Coordinates   2440984..2442036 (-) Length   350 a.a.
NCBI ID   WP_062823905.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis strain 189 isolate 189     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2435984..2447036
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A3218_RS10505 (A3218_10505) aroK 2436671..2437189 (-) 519 WP_009041824.1 shikimate kinase AroK -
  A3218_RS10510 (A3218_10510) pilQ 2437194..2439266 (-) 2073 WP_062823903.1 type IV pilus secretin PilQ Machinery gene
  A3218_RS10515 (A3218_10515) - 2439280..2439807 (-) 528 WP_062823904.1 pilus assembly protein PilP -
  A3218_RS10520 (A3218_10520) pilO 2439804..2440421 (-) 618 WP_009041821.1 type 4a pilus biogenesis protein PilO -
  A3218_RS10525 (A3218_10525) - 2440418..2440984 (-) 567 WP_009041820.1 PilN domain-containing protein -
  A3218_RS10530 (A3218_10530) pilM 2440984..2442036 (-) 1053 WP_062823905.1 pilus assembly protein PilM Machinery gene
  A3218_RS10535 (A3218_10535) - 2442253..2444688 (+) 2436 WP_202908062.1 penicillin-binding protein 1A -
  A3218_RS10540 (A3218_10540) - 2444831..2446099 (-) 1269 WP_062823907.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 37129.46 Da        Isoelectric Point: 4.9975

>NTDB_id=174877 A3218_RS10530 WP_062823905.1 2440984..2442036(-) (pilM) [Pseudomonas chlororaphis strain 189 isolate 189]
MLGLFSKKASSLLGIDISSTSVKLLELSRVGGRFRVEAYGVESLPANAVVEKNIVEPEGVGHALSRLLARARPGAKHAAL
AMAGSAVISKVIEMEAGLSDDDRESQLKVDADQYIPYPLDEAAIDFDVLGYSARNPERVDVLLAACRKEHIEVREAALEL
AGLTARVVDLEVCALERSFALAAGSPTARTVALLDIGATLTTLSVVHEGQIVYSREQLFGGHQLTEHIQRRYGLSKEEAG
QAKKQGGLPDDYASEVLQPFQEAVVQQVSRSLQFFQASAPQHQVDSILLAGGSASVPGLVPLIEQRLGLPALIANPFVGM
SIGARVDAAALASEAPSLMIACGLALRSFD

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=174877 A3218_RS10530 WP_062823905.1 2440984..2442036(-) (pilM) [Pseudomonas chlororaphis strain 189 isolate 189]
GTGCTAGGACTCTTCAGTAAAAAGGCGAGTTCCCTTCTGGGGATCGATATCAGCTCCACCTCGGTCAAGCTTCTTGAATT
GAGCCGTGTCGGTGGCCGCTTTCGGGTCGAGGCCTATGGCGTGGAGTCGTTGCCGGCCAACGCCGTGGTCGAAAAAAACA
TCGTCGAGCCTGAAGGGGTCGGGCATGCCCTGTCTCGCCTGCTGGCCAGGGCCCGGCCGGGTGCGAAGCACGCGGCGCTG
GCGATGGCCGGCTCGGCGGTGATCAGCAAGGTCATCGAGATGGAGGCCGGGCTGTCCGACGACGATCGGGAAAGCCAGCT
CAAGGTCGACGCCGACCAGTACATTCCCTACCCGCTGGACGAGGCCGCCATCGATTTCGACGTCCTCGGCTATTCGGCCC
GCAATCCCGAACGCGTCGATGTGCTGTTGGCGGCCTGTCGCAAGGAGCACATCGAAGTTCGCGAAGCGGCGCTCGAGCTC
GCCGGGCTGACGGCCCGAGTGGTGGACCTGGAAGTCTGCGCGCTGGAGCGTTCGTTCGCCCTGGCGGCCGGCTCGCCGAC
TGCCCGGACCGTGGCGCTGCTCGATATCGGCGCCACCCTGACCACCCTGAGCGTCGTGCATGAAGGGCAGATCGTCTATT
CACGTGAGCAGTTGTTCGGCGGACACCAACTGACCGAACACATCCAGCGCCGCTACGGGCTGTCGAAAGAAGAGGCCGGC
CAGGCAAAAAAGCAGGGTGGCCTGCCGGACGATTACGCCAGTGAGGTGCTGCAACCCTTTCAGGAGGCGGTCGTGCAACA
GGTTTCGCGCTCGTTGCAGTTTTTCCAGGCCTCCGCCCCGCAACACCAGGTCGATTCCATTCTGTTGGCGGGTGGCAGCG
CCTCGGTTCCGGGGCTTGTCCCGTTGATCGAGCAGCGCCTGGGCTTGCCTGCGCTGATCGCCAATCCCTTCGTTGGCATG
AGCATCGGCGCCAGGGTCGACGCCGCGGCCCTGGCTAGCGAGGCCCCGTCCTTGATGATCGCCTGCGGGCTGGCCTTGCG
GAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-349)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.398

100

0.557
  comM Acinetobacter nosocomialis M2

55.114

100

0.554
  comM Acinetobacter baylyi ADP1

52.273

100

0.526
  pilM Legionella pneumophila strain ERS1305867

44.633

100

0.451

Multiple sequence alignment