Detailed information NTG0174048

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: BSZ13_RS03440
• Genome accession: NZ_CP018206
• Coordinates: 672823..674208 (-)
• Length: 461 a.a.
• NCBI ID: WP_001550607.1
• Uniprot ID: -
• Organism: Escherichia coli strain MRSN346647
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 667823..679208

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BSZ13_RS03410 (BSZ13_03415)	mutT	668273..668671 (+)	399	WP_001550604.1	8-oxo-dGTP diphosphatase MutT	-
BSZ13_RS03415 (BSZ13_03425)	yacG	668759..668956 (-)	198	WP_000005042.1	DNA gyrase inhibitor YacG	-
BSZ13_RS03420 (BSZ13_03430)	zapD	668966..669709 (-)	744	WP_001194736.1	cell division protein ZapD	-
BSZ13_RS03425 (BSZ13_03435)	coaE	669709..670329 (-)	621	WP_001269520.1	dephospho-CoA kinase	-
BSZ13_RS28175	-	670354..670398 (+)	45	WP_120795372.1	protein YacM	-
BSZ13_RS03430 (BSZ13_03440)	guaC	670554..671597 (+)	1044	WP_001217337.1	GMP reductase	-
BSZ13_RS03435 (BSZ13_03445)	hofC	671632..672833 (-)	1202	Protein_608	protein transport protein HofC	-
BSZ13_RS03440 (BSZ13_03450)	pilB	672823..674208 (-)	1386	WP_001550607.1	type II secretion system protein GspE	Machinery gene
BSZ13_RS03445 (BSZ13_03455)	ppdD	674218..674657 (-)	440	Protein_610	prepilin peptidase-dependent pilin	-
BSZ13_RS03455 (BSZ13_03465)	nadC	674861..675754 (-)	894	WP_001135168.1	carboxylating nicotinate-nucleotide diphosphorylase	-
BSZ13_RS03460 (BSZ13_03470)	ampD	675842..676393 (+)	552	WP_001550610.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
BSZ13_RS03465 (BSZ13_03475)	ampE	676390..677244 (+)	855	WP_001550611.1	beta-lactamase regulator AmpE	-
BSZ13_RS03470 (BSZ13_03480)	aroP	677287..678660 (-)	1374	WP_000969915.1	aromatic amino acid transporter AroP	-

Sequence

Protein

• Length: 461 a.a.
• Molecular weight: 50699.41 Da
• Isoelectric Point: 6.6782

>NTDB_id=174048 BSZ13_RS03440 WP_001550607.1 672823..674208(-) (pilB) [Escherichia coli strain MRSN346647]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPRWQAPGCRHCYHGFYGRTALFE
VLPITPIIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide

• Length: 1386 bp

>NTDB_id=174048 BSZ13_RS03440 WP_001550607.1 672823..674208(-) (pilB) [Escherichia coli strain MRSN346647]
ATGAATATTCCACAGCTCACCGCCCTGTGTCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTGGTCGATGCCCCTTCGCATGAGCTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGCAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGATTGTTACAGCA
GGTGAGTCAGGCACTGGATGTCAACACGCTTGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACAGGCCCTACAGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAACGCTGAAT
ACTGCTGATATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCCGGTCTTACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGACCCTGACGTCATTATGATCGGAGAGATCC
GCGATGGCGAAACAGCAGAGATCGCTATTAAAGCGGCGCAAACCGGTCACCTGGTGTTGTCTACCCTACACACCAACTCC
ACCTGCGAAACGCTGGTGCGTTTACAGCAAATGGGCGTTGCTCGCTGGATGCTCTCATCAGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGCAAACTTTGCCCACATTGTCGCCGACAGCAAGGGGAGCCAATCCATATTCCAGACAATGTATGGC
CGTCGCCGCTGCCCCGCTGGCAAGCCCCTGGTTGTAGACACTGCTACCACGGTTTTTATGGTCGTACGGCCTTATTTGAA
GTTCTACCCATAACACCGATCATACGCCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACACACGCCCGACA
GGCGGGTATGCGTACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TATTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (8-68)

• Type II/IV secretion system protein (T2SSE) (88-353)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Legionella pneumophila strain ERS1305867	50	83.297	0.416
pilB	Glaesserella parasuis strain SC1401	41.253	100	0.414
pilB	Acinetobacter baylyi ADP1	40.385	100	0.41
pilB	Vibrio campbellii strain DS40M4	48.446	83.731	0.406
pilB	Vibrio cholerae strain A1552	46.667	84.599	0.395
pilB	Vibrio parahaemolyticus RIMD 2210633	46.667	84.599	0.395
pilB	Haemophilus influenzae 86-028NP	45.22	83.948	0.38
pilB	Haemophilus influenzae Rd KW20	44.444	83.948	0.373
pilF	Neisseria gonorrhoeae MS11	44.416	83.514	0.371
pilB	Acinetobacter baumannii D1279779	43.846	84.599	0.371
pilF	Thermus thermophilus HB27	41.278	88.286	0.364