Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   A0T30_RS04385 Genome accession   NZ_CP014784
Coordinates   959555..961666 (+) Length   703 a.a.
NCBI ID   WP_061903210.1    Uniprot ID   -
Organism   Aquipseudomonas alcaligenes strain NEB 585     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 954555..966666
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A0T30_RS04365 (A0T30_04365) pilM 956650..957714 (+) 1065 WP_061903206.1 pilus assembly protein PilM Machinery gene
  A0T30_RS04370 (A0T30_04370) - 957714..958343 (+) 630 WP_061903207.1 PilN domain-containing protein -
  A0T30_RS04375 (A0T30_04375) pilO 958340..958978 (+) 639 WP_061903208.1 type 4a pilus biogenesis protein PilO -
  A0T30_RS04380 (A0T30_04380) pilP 958975..959502 (+) 528 WP_061903209.1 type 4a pilus biogenesis lipoprotein PilP -
  A0T30_RS04385 (A0T30_04385) pilQ 959555..961666 (+) 2112 WP_061903210.1 type IV pilus secretin PilQ Machinery gene
  A0T30_RS04390 (A0T30_04390) aroK 961669..962187 (+) 519 WP_061903211.1 shikimate kinase AroK -
  A0T30_RS04395 (A0T30_04395) aroB 962237..963340 (+) 1104 WP_061905626.1 3-dehydroquinate synthase -
  A0T30_RS04400 (A0T30_04400) - 963350..965002 (+) 1653 WP_061903212.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 703 a.a.        Molecular weight: 76055.35 Da        Isoelectric Point: 4.6062

>NTDB_id=174022 A0T30_RS04385 WP_061903210.1 959555..961666(+) (pilQ) [Aquipseudomonas alcaligenes strain NEB 585]
MIGMLSRLGVSLVLASLAPAVLAAELQKLDVAALPGDKIELKLSFDQPVSSPRGYTIEQPARIALDLPGVSNGLGVKNRE
LGVGNARSVTVVEAKDRTRLIVNLTTLVPYSTRAEGNNLYLLVGDAAVRSDTVASSPVAAKPLVAAPAGRAIRGIDFQRG
EQGEGNVVIELSDPSVNPDIQEQGGKIRLNFSKTQLPENLRVRLDVKDFATPVQFVSASDQDGLSTISIEPVGFYDYLAF
QADNKLTISVKPLSQDDVEKRKKERFAYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITLRLQNVPWDQAL
DLVLKTRGLDKRKVGNVLLIAPADEIAARERQELEAQNQIAELAPLRRELIQVNYAKAADIATLFQSVAATEGGEAGERG
TVTVDDRTNSIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVGYEKDIGVRWGGTISNGKWSTYGKDGAQGIKD
EEGFSCGPFAGQCTFPTVKNGDAQLPVPFVDMGATDASSGIGIGFLTDNVLLDLQLSAMEASGNGEIISQPKVVTSDKET
AKILKGAEIPYQEASSSGATTISFKEAALSLEVTPQITPDNRIIMEVKVNKDEPDYDREVNGVPPINKNEVNAKVLVEDG
ETIVIGGVFSNTQSKVVEKVPLLGDVPFIGRLFRRDAVRDQKNELLVFITPRIMNNQSIAVSN

Nucleotide


Download         Length: 2112 bp        

>NTDB_id=174022 A0T30_RS04385 WP_061903210.1 959555..961666(+) (pilQ) [Aquipseudomonas alcaligenes strain NEB 585]
ATGATCGGCATGCTTTCTCGCCTCGGGGTCTCCCTCGTCCTTGCTTCGCTCGCACCAGCTGTTCTGGCTGCTGAGCTGCA
GAAGCTTGATGTGGCCGCGTTGCCAGGTGACAAGATCGAGCTGAAATTGTCCTTTGATCAGCCGGTTTCCAGTCCTCGTG
GTTACACCATTGAGCAACCGGCGCGAATTGCTTTGGATCTTCCAGGTGTTTCCAATGGTCTGGGGGTAAAGAATCGTGAG
CTGGGTGTTGGTAATGCGCGCAGTGTGACGGTAGTCGAGGCTAAGGATCGAACCAGGTTGATTGTCAACCTGACTACACT
GGTTCCCTACAGCACTCGTGCCGAAGGTAACAATCTCTACCTGCTGGTAGGTGATGCTGCTGTGCGCTCCGATACCGTCG
CCTCGTCGCCCGTGGCTGCTAAGCCTCTGGTCGCTGCGCCGGCTGGTCGAGCCATTCGCGGGATTGATTTCCAGCGGGGC
GAGCAGGGTGAGGGTAACGTCGTTATCGAGCTTTCAGACCCCTCCGTGAATCCTGATATTCAGGAGCAGGGTGGTAAAAT
TCGCTTGAATTTCTCCAAGACCCAGCTGCCAGAGAACTTGCGAGTTCGTCTGGATGTGAAGGACTTTGCGACGCCGGTGC
AGTTCGTGAGTGCTTCGGATCAGGATGGGCTATCAACTATCTCCATTGAGCCTGTTGGGTTCTATGACTATCTGGCTTTC
CAGGCGGACAATAAGCTGACTATCAGTGTCAAGCCGCTAAGCCAGGATGATGTTGAGAAGCGGAAGAAGGAGCGTTTCGC
CTACAGTGGCGAAAAGCTTTCTCTTAACTTCCAAGACATTGATGTTCGCTCGGTCCTGCAGCTGATTGCAGACTTCACCG
ACTTGAACCTGGTTGCCAGTGACACGGTACAGGGCAACATCACGCTGCGTCTGCAAAACGTACCTTGGGATCAGGCTCTC
GATCTGGTGTTGAAAACCCGGGGGCTTGACAAGCGTAAGGTTGGCAACGTTCTGCTGATCGCTCCTGCTGATGAGATTGC
TGCCAGAGAGCGCCAGGAGCTTGAAGCACAAAATCAAATTGCGGAACTTGCGCCTTTGCGCCGTGAGTTGATTCAGGTCA
ATTATGCCAAAGCTGCGGATATTGCCACTTTGTTCCAGTCGGTTGCTGCGACTGAAGGCGGCGAAGCTGGTGAGCGCGGC
ACTGTCACTGTGGACGACAGAACCAACAGCATCATTGCCTATCAGACGCAGGATCGCTTGGATGAGCTGCGTCGAATTGT
GGCTCAGCTGGATATTCCTGTGCGTCAGGTAATGATTGAGGCGCGCATCGTCGAGGCCAACGTCGGCTATGAGAAGGATA
TCGGTGTGCGTTGGGGGGGGACAATCAGTAATGGCAAGTGGTCCACCTATGGTAAGGATGGCGCCCAAGGTATCAAGGAT
GAAGAAGGTTTCTCGTGCGGGCCCTTTGCCGGGCAATGTACCTTCCCGACCGTAAAGAACGGTGATGCACAGCTTCCAGT
TCCTTTCGTTGATATGGGGGCTACTGACGCCAGCTCTGGCATCGGTATCGGTTTCCTTACTGACAATGTGCTGTTGGATT
TGCAGCTGAGTGCCATGGAGGCCAGCGGTAATGGCGAGATTATTTCCCAGCCCAAGGTTGTTACTTCTGACAAGGAAACT
GCAAAAATCCTGAAAGGTGCGGAGATTCCTTATCAGGAAGCCAGTTCCAGTGGTGCGACGACTATTTCCTTTAAAGAGGC
CGCGCTCTCTCTGGAGGTGACTCCGCAGATTACGCCGGATAACCGCATCATCATGGAGGTCAAGGTTAATAAAGACGAGC
CTGACTATGATCGTGAGGTCAATGGTGTTCCGCCTATCAACAAGAATGAGGTCAATGCCAAAGTTCTTGTGGAAGATGGC
GAGACCATTGTGATCGGTGGCGTGTTCTCCAATACTCAGTCCAAGGTCGTTGAGAAAGTCCCGCTGCTGGGGGATGTGCC
GTTTATCGGTCGTCTGTTCCGCCGCGATGCGGTAAGGGATCAGAAGAACGAACTGCTGGTCTTCATTACTCCGCGGATCA
TGAACAACCAGTCAATCGCGGTAAGCAACTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(538-694)

Bacterial type II/III secretion system short domain (Secretin_N)

(370-436)

AMIN domain (AMIN)

(37-120)

Secretin and TonB N terminus short domain (STN)

(296-342)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

76.197

100

0.77
  comQ Acinetobacter baylyi ADP1

42.014

98.862

0.415
  pilQ Acinetobacter baumannii strain A118

40.563

100

0.41
  pilQ Acinetobacter baumannii D1279779

40.563

100

0.41
  pilQ Legionella pneumophila strain ERS1305867

39.143

96.302

0.377
  pilQ Legionella pneumophila strain Lp02

38.304

97.297

0.373

Multiple sequence alignment