Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   A0T30_RS04365 Genome accession   NZ_CP014784
Coordinates   956650..957714 (+) Length   354 a.a.
NCBI ID   WP_061903206.1    Uniprot ID   A0AA42MZ32
Organism   Aquipseudomonas alcaligenes strain NEB 585     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 951650..962714
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A0T30_RS04355 (A0T30_04355) - 952721..953989 (+) 1269 WP_061903204.1 malic enzyme-like NAD(P)-binding protein -
  A0T30_RS04360 (A0T30_04360) - 954051..956465 (-) 2415 WP_373869750.1 penicillin-binding protein 1A -
  A0T30_RS04365 (A0T30_04365) pilM 956650..957714 (+) 1065 WP_061903206.1 pilus assembly protein PilM Machinery gene
  A0T30_RS04370 (A0T30_04370) - 957714..958343 (+) 630 WP_061903207.1 PilN domain-containing protein -
  A0T30_RS04375 (A0T30_04375) pilO 958340..958978 (+) 639 WP_061903208.1 type 4a pilus biogenesis protein PilO -
  A0T30_RS04380 (A0T30_04380) pilP 958975..959502 (+) 528 WP_061903209.1 type 4a pilus biogenesis lipoprotein PilP -
  A0T30_RS04385 (A0T30_04385) pilQ 959555..961666 (+) 2112 WP_061903210.1 type IV pilus secretin PilQ Machinery gene
  A0T30_RS04390 (A0T30_04390) aroK 961669..962187 (+) 519 WP_061903211.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37952.32 Da        Isoelectric Point: 4.4244

>NTDB_id=174021 A0T30_RS04365 WP_061903206.1 956650..957714(+) (pilM) [Aquipseudomonas alcaligenes strain NEB 585]
MLGLFNKKANTLLGIDISSTSVKLLELSRSGSRYKVEAYAVEPLPPNAVVEKNIAELEGVGQALARVLSKAKTSVRTAAV
AVAGSAVITKTIEMEAGLNDDELENQLKIEADQYIPYPLEEVAIDFEVQGLSPRNPERVEVLLAACRKENVEVREAALGL
AGVTAKVVDVEAYALERAYGLLESQLGGSVDELTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYESEVLQPFKEAVVQQVSRSLQFFFAAGQFNDVDYILLAGGTASIPDLDRMIQQKIGTPTLVANP
FADMALGSKVNAGALASDAPSLMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=174021 A0T30_RS04365 WP_061903206.1 956650..957714(+) (pilM) [Aquipseudomonas alcaligenes strain NEB 585]
GTGCTAGGGCTCTTCAATAAGAAAGCGAACACGCTTCTGGGGATCGACATCAGTTCGACCTCGGTCAAGCTCCTTGAATT
GAGTCGCTCCGGAAGCCGCTACAAGGTAGAGGCCTATGCTGTCGAGCCGCTCCCACCCAATGCTGTTGTCGAAAAGAACA
TCGCCGAGCTGGAGGGGGTCGGTCAGGCTCTTGCGCGCGTTCTGAGCAAGGCGAAAACCAGCGTGCGTACTGCGGCGGTG
GCGGTGGCCGGCTCTGCGGTGATCACCAAGACCATCGAGATGGAAGCCGGTCTCAACGATGACGAGCTGGAGAACCAGCT
GAAGATAGAGGCCGACCAGTACATCCCCTATCCATTGGAAGAGGTGGCTATCGACTTCGAGGTGCAGGGCCTTTCGCCGC
GCAACCCCGAACGAGTCGAAGTCCTGCTGGCTGCCTGCCGTAAAGAGAACGTCGAGGTTCGCGAGGCGGCTCTTGGGCTC
GCCGGTGTTACGGCCAAGGTTGTCGATGTGGAAGCTTATGCGCTCGAGCGCGCCTATGGCCTGCTCGAGTCCCAGCTTGG
CGGTAGCGTCGATGAGCTGACGGTTGCCGTGGTCGACATCGGTGCCACCATGACCACCCTGAGCGTGCTGCACAATGGCC
GCACGATCTACACCCGGGAGCAGCTGTTCGGTGGGCGTCAGCTGACCGAAGAGATTCAGCGCCGCTACGGTCTCTCCGTC
GAGGAGGCCGGTCTGGCCAAGAAACAGGGTGGCCTGCCGGATGACTACGAAAGCGAAGTGCTGCAGCCCTTCAAGGAAGC
GGTCGTGCAGCAGGTTTCGCGTTCGCTGCAATTCTTCTTCGCGGCCGGTCAGTTCAACGATGTCGACTACATCCTGCTGG
CAGGTGGTACGGCCTCGATTCCCGATCTGGATCGGATGATTCAACAGAAGATTGGTACTCCTACCCTGGTGGCCAACCCC
TTCGCCGACATGGCGCTGGGAAGCAAGGTCAATGCCGGAGCCCTGGCTAGCGATGCCCCGTCCTTGATGATTGCCTGTGG
CCTGGCGATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.932

100

0.559
  comM Acinetobacter nosocomialis M2

55.932

100

0.559
  comM Acinetobacter baylyi ADP1

53.107

100

0.531
  pilM Legionella pneumophila strain ERS1305867

45.763

100

0.458

Multiple sequence alignment