Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC   Type   Machinery gene
Locus tag   A0V43_RS10065 Genome accession   NZ_CP014749
Coordinates   2058269..2058565 (-) Length   98 a.a.
NCBI ID   WP_015375395.1    Uniprot ID   L8A1X1
Organism   Geobacillus sp. JS12     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2053269..2063565
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A0V43_RS10030 (A0V43_10030) - 2053872..2055536 (+) 1665 WP_063193028.1 SNF2-related protein -
  A0V43_RS10035 (A0V43_10035) - 2055523..2056317 (+) 795 WP_042380976.1 YqhG family protein -
  A0V43_RS10040 (A0V43_10040) - 2056420..2056599 (-) 180 WP_011231910.1 YqzE family protein -
  A0V43_RS10045 (A0V43_10045) comGG 2056645..2057034 (-) 390 WP_012820555.1 competence type IV pilus minor pilin ComGG -
  A0V43_RS10050 (A0V43_10050) comGF 2057109..2057597 (-) 489 WP_335339946.1 competence type IV pilus minor pilin ComGF -
  A0V43_RS20930 (A0V43_10055) - 2057534..2057859 (-) 326 Protein_2088 type II secretion system protein -
  A0V43_RS10060 (A0V43_10060) - 2057899..2058285 (-) 387 WP_236930795.1 type II secretion system protein -
  A0V43_RS10065 (A0V43_10065) comGC 2058269..2058565 (-) 297 WP_015375395.1 competence type IV pilus major pilin ComGC Machinery gene
  A0V43_RS10070 (A0V43_10070) - 2058797..2060454 (+) 1658 Protein_2091 IS1634 family transposase -
  A0V43_RS10075 (A0V43_10075) comGB 2060644..2061672 (-) 1029 WP_063193030.1 competence type IV pilus assembly protein ComGB -
  A0V43_RS10080 (A0V43_10080) comGA 2061669..2062742 (-) 1074 WP_015375397.1 competence type IV pilus ATPase ComGA Machinery gene

Sequence

Protein


Download         Length: 98 a.a.        Molecular weight: 10761.58 Da        Isoelectric Point: 7.2422

>NTDB_id=173590 A0V43_RS10065 WP_015375395.1 2058269..2058565(-) (comGC) [Geobacillus sp. JS12]
MNQKGFTLIEMLIVMMVISVLLLIAIPNITKHNSMINSKGCEAFLNTVQAQVKAYEMEHNKIPTVQELLAGRYIKSDKCP
NGHAIQISTNGDVSESGS

Nucleotide


Download         Length: 297 bp        

>NTDB_id=173590 A0V43_RS10065 WP_015375395.1 2058269..2058565(-) (comGC) [Geobacillus sp. JS12]
ATGAATCAAAAGGGATTCACATTGATCGAAATGTTGATCGTGATGATGGTGATTTCTGTTCTGTTGCTGATTGCCATTCC
GAATATAACAAAGCACAACAGCATGATCAATTCGAAAGGATGCGAGGCGTTTTTGAACACCGTGCAAGCGCAGGTGAAAG
CGTATGAGATGGAGCATAACAAAATTCCGACGGTGCAGGAATTGCTCGCTGGCCGCTATATCAAGTCGGACAAATGTCCG
AACGGTCATGCGATCCAAATCAGCACGAACGGCGATGTGAGTGAAAGTGGCTCGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB L8A1X1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Bacillus subtilis subsp. subtilis str. 168

53.125

97.959

0.52
  comGC Staphylococcus aureus MW2

43.011

94.898

0.408
  comGC Staphylococcus aureus N315

43.011

94.898

0.408

Multiple sequence alignment