Detailed information NTG0173591

Bioinformatically predicted

Overview

• Name: comGA
• Type: Machinery gene
• Locus tag: A0V43_RS10080
• Genome accession: NZ_CP014749
• Coordinates: 2061669..2062742 (-)
• Length: 357 a.a.
• NCBI ID: WP_015375397.1
• Uniprot ID: L7ZU71
• Organism: Geobacillus sp. JS12
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2056669..2067742

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A0V43_RS10050 (A0V43_10050)	comGF	2057109..2057597 (-)	489	WP_335339946.1	competence type IV pilus minor pilin ComGF	-
A0V43_RS20930 (A0V43_10055)	-	2057534..2057859 (-)	326	Protein_2088	type II secretion system protein	-
A0V43_RS10060 (A0V43_10060)	-	2057899..2058285 (-)	387	WP_236930795.1	type II secretion system protein	-
A0V43_RS10065 (A0V43_10065)	comGC	2058269..2058565 (-)	297	WP_015375395.1	competence type IV pilus major pilin ComGC	Machinery gene
A0V43_RS10070 (A0V43_10070)	-	2058797..2060454 (+)	1658	Protein_2091	IS1634 family transposase	-
A0V43_RS10075 (A0V43_10075)	comGB	2060644..2061672 (-)	1029	WP_063193030.1	competence type IV pilus assembly protein ComGB	-
A0V43_RS10080 (A0V43_10080)	comGA	2061669..2062742 (-)	1074	WP_015375397.1	competence type IV pilus ATPase ComGA	Machinery gene
A0V43_RS10085 (A0V43_10085)	-	2062935..2063633 (+)	699	WP_015375398.1	helix-turn-helix domain-containing protein	-
A0V43_RS10090 (A0V43_10090)	-	2063789..2064031 (+)	243	WP_008879854.1	DUF2626 family protein	-
A0V43_RS10095 (A0V43_10095)	-	2064115..2065247 (-)	1133	Protein_2096	SAM-dependent methyltransferase	-
A0V43_RS10100 (A0V43_10100)	-	2065567..2066199 (-)	633	WP_063193031.1	MBL fold metallo-hydrolase	-
A0V43_RS19065	-	2066368..2066544 (+)	177	WP_011231921.1	DUF2759 domain-containing protein	-
A0V43_RS10105 (A0V43_10105)	-	2066580..2067740 (-)	1161	WP_063193032.1	M14 family metallocarboxypeptidase	-

Sequence

Protein

• Length: 357 a.a.
• Molecular weight: 39640.10 Da
• Isoelectric Point: 8.1836

>NTDB_id=173591 A0V43_RS10080 WP_015375397.1 2061669..2062742(-) (comGA) [Geobacillus sp. JS12]
MLNEIEQTANRLLAEAVQRRASDLHLVPRRRDAAVRLRLDGMLVDVGALPKETAERLIVHFKFLAGMDIGERRRPQSGAM
EVAEFGETVYLRLSTLPTLYDESLVIRLLPQRLSLPLRELSLFFHSTARLFSFMQHPQGLVLLTGPTGSGKTTTLYTLLD
LCQAERQRNIITLEDPIEKQNERLLQVQINEKAGITYAAGLKAALRHDPDVLMVGEIRDHDTAAIAIRSALSGHLVVSTM
HAADAVGAVYRLHEFGIPLGDLAETLLAVSAQRLVELCCPLCGDDCHPSCGRLGRRRRTAVYELLCGSALEDVIHFLSNG
RGKPKRSYMTLARLIRKGIALGYLPVRMLELVGGEER

Nucleotide

• Length: 1074 bp

>NTDB_id=173591 A0V43_RS10080 WP_015375397.1 2061669..2062742(-) (comGA) [Geobacillus sp. JS12]
GTGCTGAACGAGATCGAACAAACGGCAAACCGCCTCCTCGCTGAAGCGGTGCAGCGCCGCGCCTCGGATCTCCACCTCGT
TCCGCGCCGCCGTGATGCCGCCGTTCGTCTTCGGCTCGATGGCATGCTTGTCGACGTTGGCGCTCTTCCGAAAGAAACGG
CGGAACGCCTCATCGTTCACTTTAAATTTTTAGCCGGCATGGACATCGGGGAACGACGCCGTCCGCAAAGCGGCGCCATG
GAAGTAGCGGAGTTTGGGGAAACGGTGTATTTGCGTTTATCGACATTGCCGACGCTCTACGATGAAAGCCTCGTCATTCG
ACTTCTGCCGCAGCGCCTCTCGCTGCCGCTTCGCGAACTATCCTTATTCTTCCACTCCACCGCACGATTATTTTCCTTCA
TGCAGCATCCGCAAGGACTCGTGCTGTTGACCGGTCCGACGGGGTCAGGAAAAACGACGACCCTGTACACCCTTCTTGAT
CTTTGTCAAGCGGAAAGGCAGCGCAATATCATCACGCTTGAAGATCCAATCGAAAAACAGAACGAACGACTGTTGCAAGT
GCAAATCAATGAAAAAGCAGGCATCACGTACGCAGCTGGGTTAAAAGCGGCGCTGCGTCATGACCCGGATGTGTTGATGG
TCGGCGAGATCCGCGATCATGACACGGCGGCCATTGCCATCCGTTCAGCATTGAGCGGCCATTTGGTCGTATCGACGATG
CACGCCGCCGATGCAGTCGGCGCCGTCTACCGGCTGCATGAATTCGGCATTCCGCTCGGCGATTTGGCTGAGACGCTGCT
TGCCGTCTCTGCCCAGCGGCTTGTCGAGCTTTGCTGCCCGTTGTGCGGCGACGATTGCCATCCATCGTGCGGCCGTCTTG
GGCGCCGCCGGCGCACGGCTGTTTATGAGTTGCTTTGCGGTTCAGCCCTGGAGGACGTGATTCATTTTCTCTCCAATGGG
AGAGGGAAGCCCAAGCGCTCTTATATGACGCTCGCTCGCTTGATCCGCAAGGGGATTGCGCTCGGCTACCTGCCCGTCCG
CATGCTTGAGCTCGTCGGAGGGGAAGAAAGATGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (12-276)

3D structure

• AlphaFold DB: L7ZU71

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGA	Bacillus subtilis subsp. subtilis str. 168	50.581	96.359	0.487