Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   A0U95_RS14000 Genome accession   NZ_CP014693
Coordinates   3257564..3259654 (-) Length   696 a.a.
NCBI ID   WP_013692093.1    Uniprot ID   F2KDE9
Organism   Pseudomonas brassicacearum strain L13-6-12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3252564..3264654
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A0U95_RS13985 (A0U95_13960) - 3254202..3255782 (-) 1581 WP_003196987.1 AAA family ATPase -
  A0U95_RS13990 (A0U95_13965) aroB 3255792..3256892 (-) 1101 WP_003196985.1 3-dehydroquinate synthase -
  A0U95_RS13995 (A0U95_13970) aroK 3257041..3257559 (-) 519 WP_003196983.1 shikimate kinase AroK -
  A0U95_RS14000 (A0U95_13975) pilQ 3257564..3259654 (-) 2091 WP_013692093.1 type IV pilus secretin PilQ Machinery gene
  A0U95_RS14005 (A0U95_13980) - 3259668..3260195 (-) 528 WP_003196979.1 pilus assembly protein PilP -
  A0U95_RS14010 (A0U95_13985) pilO 3260192..3260815 (-) 624 WP_003196977.1 type 4a pilus biogenesis protein PilO -
  A0U95_RS14015 (A0U95_13990) - 3260812..3261378 (-) 567 WP_003196975.1 PilN domain-containing protein -
  A0U95_RS14020 (A0U95_13995) comM 3261378..3262442 (-) 1065 WP_003196973.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 696 a.a.        Molecular weight: 75130.19 Da        Isoelectric Point: 5.2961

>NTDB_id=173289 A0U95_RS14000 WP_013692093.1 3257564..3259654(-) (pilQ) [Pseudomonas brassicacearum strain L13-6-12]
MNRFFSALGISLWIALLSPMVLGASLKTLDVAALPGDRVELKLSFDGPPPSPRGYTTEQPARIALDLPGVTNQLSSKRRD
LGGGNARSATVVEAGDRTRLIIGLTQLAPYDTRIEGNNLFVVVGKGAALKSTAPKNSTTQAPRPAVASAAPARASAPVGK
AIRGVDFQRGTQGEGNVVIDLSDPSIAPDIQEREGKIILNFARTQLPEPLRVRLDVKDFATPVQFVNASAGSDRATISIE
PSGTFDYSTYQTDNKLTVSVRPMTVDDLQKRNADRAAYSGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLR
LQNVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLAPLRRELLQVNYAKATDIAKLFQSVTS
AEAKADERGTITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVQNKGNWSSS
GVTTGDSTTVGTPGSTSTNQPFVDLGASGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGT
EIPYQEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVNDVPPIKKNEVNAKVLVNDGETIVIGG
VFSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSDSKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2091 bp        

>NTDB_id=173289 A0U95_RS14000 WP_013692093.1 3257564..3259654(-) (pilQ) [Pseudomonas brassicacearum strain L13-6-12]
ATGAACAGGTTTTTTTCAGCCCTCGGTATTTCGCTATGGATAGCGCTGTTGTCGCCGATGGTTCTCGGGGCCAGCCTGAA
GACGTTGGATGTGGCGGCGTTGCCGGGGGACCGTGTCGAGTTGAAGCTCTCCTTCGACGGGCCGCCGCCGTCACCCCGGG
GCTACACCACCGAACAACCGGCGCGGATTGCGCTGGACCTGCCGGGCGTGACCAATCAACTTTCCAGCAAGCGCCGCGAC
CTGGGCGGGGGCAATGCACGCAGCGCCACTGTGGTCGAGGCTGGGGATCGTACTCGGTTGATCATCGGCCTTACCCAGCT
GGCGCCGTATGACACCCGGATCGAAGGTAACAACCTGTTCGTGGTGGTTGGCAAGGGCGCCGCCCTGAAGAGCACTGCCC
CGAAGAACTCCACCACCCAGGCCCCGCGCCCGGCGGTTGCCAGCGCTGCGCCTGCGAGGGCCAGTGCGCCGGTGGGCAAG
GCGATCCGTGGGGTGGACTTCCAGCGCGGGACCCAGGGCGAAGGCAACGTGGTGATCGACCTGTCTGATCCGTCCATTGC
CCCGGACATCCAGGAGCGCGAAGGCAAGATCATCCTCAATTTCGCCAGGACCCAGTTGCCCGAGCCGCTGCGGGTGCGCC
TGGACGTCAAGGACTTCGCCACCCCGGTGCAATTCGTCAATGCCAGCGCCGGCAGCGACCGGGCGACCATCAGCATCGAA
CCCAGCGGCACCTTCGACTACTCGACCTACCAGACCGACAACAAGCTCACCGTCAGCGTCCGGCCCATGACCGTCGACGA
CCTGCAAAAACGCAACGCCGACCGCGCGGCCTACAGTGGCGAGAAGCTCTCGCTCAACTTCCAGGACATCGAGGTGCGTT
CGGTCCTGCAACTGATTGCCGATTTTACCAACCTGAACCTCGTGGCCAGCGACACGGTGCAGGGTGGCATCACCCTGCGG
TTGCAGAATGTGCCCTGGGACCAGGCGCTCGACCTGGTGCTCAAGACCAAGGGGTTGGACAAGCGCAAGGTCGGCAACGT
GCTGTTGGTGGCTCCGGCCGATGAAATTGCCGCTCGTGAGCGCCAGGAACTGGAGTCGCAGAAGCAGATTGCCGACCTGG
CGCCGCTGCGGCGTGAGCTGTTGCAGGTCAACTATGCGAAGGCGACGGACATCGCCAAGCTGTTCCAGTCGGTGACCAGT
GCCGAGGCCAAAGCCGATGAGCGCGGCACGATCACGGTCGATGAGCGGACCAACAACATCATTGCCTACCAGACCCAGGA
CCGTCTCGACGAACTGCGCCGGATCGTGGCGCAACTGGATATTCCGGTGCGCCAGGTGATGATTGAGGCGCGGATTGTCG
AAGCCAACGTCGACTACGACAAAAGCCTGGGTGTGCGCTGGGGCGGTTCGGTTCAAAACAAGGGCAACTGGAGCTCCTCG
GGGGTTACCACGGGCGATTCCACCACCGTTGGCACGCCGGGTAGCACCAGCACCAACCAGCCGTTCGTCGACCTGGGTGC
CTCGGGCAATACCTCGGGCATCGGCATTGCCTTCATCACCGACAACGTATTGCTGGACCTTGAGCTGACGGCCATGGAGA
AAACCGGCAACGGGGAGATCGTCTCCCAGCCCAAGGTGGTGACGTCCGACAAGGAGACGGCAAAGATCCTCAAGGGCACG
GAAATTCCTTATCAGGAAGCCAGCTCCAGCGGCGCTACGTCGGTGTCGTTCAAGGAGGCTTCGCTGTCGCTGGAGGTGAC
CCCGCAGATCACTCCAGACAACCGGATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGACTACCTGAACAAGGTGA
ATGATGTACCGCCGATCAAGAAAAACGAGGTCAATGCCAAGGTCCTGGTCAATGATGGCGAGACCATTGTGATTGGCGGG
GTTTTCTCAAATACTCAGAGCAAGGTTGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCG
GCGTGATGTGGTTTCGGATAGCAAATCCGAGCTGCTGGTGTTCCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTG
TGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(531-687)

Secretin and TonB N terminus short domain (STN)

(306-352)

Bacterial type II/III secretion system short domain (Secretin_N)

(380-445)

AMIN domain (AMIN)

(37-121)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F2KDE9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.398

100

0.757
  pilQ Acinetobacter baumannii strain A118

38.71

100

0.414
  pilQ Acinetobacter baumannii D1279779

38.71

100

0.414
  comQ Acinetobacter baylyi ADP1

40.805

100

0.408
  pilQ Legionella pneumophila strain ERS1305867

38.201

97.414

0.372
  pilQ Legionella pneumophila strain Lp02

37.794

97.701

0.369

Multiple sequence alignment