Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PG2T_RS12795 Genome accession   NZ_CP014671
Coordinates   2692143..2693861 (-) Length   572 a.a.
NCBI ID   WP_068808361.1    Uniprot ID   A0A1B1YY22
Organism   Immundisolibacter cernigliae strain TR3.2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2687143..2698861
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PG2T_RS12770 (PG2T_12785) - 2687859..2688521 (+) 663 WP_145931090.1 toluene tolerance protein -
  PG2T_RS15605 - 2688467..2688700 (-) 234 WP_083214921.1 DNA gyrase inhibitor YacG -
  PG2T_RS12775 (PG2T_12790) zapD 2688637..2689410 (-) 774 WP_068806223.1 cell division protein ZapD -
  PG2T_RS12780 (PG2T_12795) coaE 2689446..2690048 (-) 603 WP_068806226.1 dephospho-CoA kinase -
  PG2T_RS12785 (PG2T_12800) pilD 2690048..2690887 (-) 840 WP_418268541.1 prepilin peptidase Machinery gene
  PG2T_RS12790 (PG2T_12805) pilC 2690899..2692134 (-) 1236 WP_068806232.1 type II secretion system F family protein Machinery gene
  PG2T_RS12795 (PG2T_12810) pilB 2692143..2693861 (-) 1719 WP_068808361.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PG2T_RS12800 (PG2T_12815) - 2694185..2695174 (+) 990 WP_145931092.1 hypothetical protein -
  PG2T_RS12805 (PG2T_12820) - 2695286..2696371 (+) 1086 WP_068806236.1 efflux RND transporter periplasmic adaptor subunit -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62430.59 Da        Isoelectric Point: 5.2373

>NTDB_id=173198 PG2T_RS12795 WP_068808361.1 2692143..2693861(-) (pilB) [Immundisolibacter cernigliae strain TR3.2]
MATPSTRLSLIGLAKRLARDGLLSEAEAAHASDIAAARETPLVAYLVETTGLDAAQIALCASSEFGLPVFDLDAFDAELL
SLHGVSDQIIRKYRALPLFRRGNRLFAAVSDPTDYRALDEIKFSSGLSTEAVLVPHDKLSALLTKVLANSENQPLDEIND
ADLDALVVQDEENDPGAGTSNIDVDDAPVVRYVNKLLLDAISKGASDLHFEPYEKRYRVRYRVDGILREMAAPPPGLANR
LAARIKVLARLDIAERRVPQDGRIKLKVSRNRAVDLRVSTCPTLYGEKIVMRVLDGGNGLLTVDELGFNPAQRELFEHNL
QKPYGMVLITGPTGSGKTMTLYAGLKELNQPGINISTAEDPVEIVMEGVNQVNVNVKAGLTFAAALRSFLRQDPDVILIG
EIRDLETAEIAVKAAQTGHMVLATLHTNDAPQTITRLINMGVPSYNVAAALNLIMAQRLARRLCKVCKRPLELPRQTLLK
EGFGEEEIDAGLTIYEAVGCDRCTDGYKGRIGIYQVMTISDDMRKLITREASDIEIAAQAKHEGIADLIQAGLDKVREGV
TSLSEIHRITKE

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=173198 PG2T_RS12795 WP_068808361.1 2692143..2693861(-) (pilB) [Immundisolibacter cernigliae strain TR3.2]
ATGGCAACTCCCAGCACCCGACTGAGCCTGATCGGGCTCGCCAAGCGCCTTGCCAGGGATGGACTGCTGAGCGAGGCAGA
GGCGGCGCACGCCAGCGATATCGCGGCGGCCCGCGAGACACCCCTCGTAGCCTACCTGGTCGAGACGACAGGACTGGACG
CGGCGCAGATCGCGCTGTGCGCCTCCAGCGAGTTCGGTCTTCCGGTGTTCGACCTGGACGCCTTCGACGCCGAGCTGCTC
AGTCTGCACGGCGTGAGCGACCAGATCATCCGCAAATACCGCGCCCTGCCGCTTTTCCGGCGCGGCAACCGGCTGTTTGC
GGCGGTGTCCGATCCGACCGACTACCGGGCCCTGGACGAGATCAAGTTCAGCTCCGGCCTGAGCACCGAGGCAGTGCTGG
TGCCGCACGACAAGCTGTCGGCCCTGCTGACCAAGGTGCTTGCCAACTCCGAAAACCAGCCGCTGGACGAAATCAACGAC
GCCGACCTGGACGCCCTGGTCGTGCAGGACGAGGAAAACGACCCGGGCGCCGGCACCAGCAACATCGACGTCGACGACGC
CCCGGTCGTGCGCTACGTCAACAAACTGCTGCTGGATGCGATCAGCAAGGGCGCTTCCGACCTGCATTTCGAACCGTATG
AGAAGCGCTATCGGGTCCGCTACCGCGTGGACGGCATCCTGCGCGAGATGGCGGCGCCACCGCCGGGCCTGGCCAATCGC
CTGGCGGCCCGCATCAAGGTGCTGGCGCGCCTGGACATTGCCGAGCGACGCGTGCCGCAGGACGGACGCATCAAGCTGAA
GGTGTCGCGCAACCGGGCGGTGGATCTTCGTGTCAGCACCTGCCCGACCCTGTACGGCGAGAAGATCGTCATGCGCGTTC
TGGACGGTGGCAACGGACTGCTGACCGTCGACGAGCTGGGATTCAACCCGGCTCAGCGGGAGCTGTTCGAACACAACCTG
CAAAAGCCATACGGCATGGTGCTGATCACCGGCCCCACCGGCAGCGGCAAAACGATGACGCTGTATGCCGGGCTGAAGGA
ACTGAACCAACCCGGCATCAACATTTCGACCGCTGAAGACCCGGTCGAGATCGTCATGGAAGGGGTCAACCAGGTCAACG
TCAACGTCAAGGCCGGTCTCACCTTTGCGGCGGCCCTGCGATCGTTCCTGCGCCAGGATCCGGACGTGATCCTGATCGGC
GAGATTCGTGATCTTGAAACGGCCGAGATTGCGGTCAAGGCAGCCCAGACCGGACACATGGTGCTGGCCACGCTGCACAC
CAACGACGCCCCGCAGACCATCACGCGCCTGATCAACATGGGCGTGCCATCCTACAACGTGGCCGCTGCGCTGAACCTGA
TCATGGCGCAGCGTCTGGCGCGGCGCCTGTGCAAGGTATGCAAGCGACCACTGGAGTTGCCACGCCAGACCCTGCTCAAG
GAAGGTTTCGGCGAGGAAGAAATCGACGCCGGTCTGACGATCTATGAAGCGGTCGGCTGCGACCGCTGTACCGACGGCTA
CAAGGGGCGTATCGGTATCTATCAGGTCATGACGATCTCGGATGACATGCGCAAGCTCATCACGCGCGAAGCCTCCGACA
TCGAAATCGCTGCCCAGGCCAAACACGAAGGCATCGCCGACTTGATACAGGCCGGTCTGGACAAGGTCCGCGAGGGCGTC
ACCAGCCTGAGCGAGATCCACCGCATCACCAAGGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)

Type II/IV secretion system protein (T2SSE)

(195-462)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B1YY22

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

51.832

100

0.519
  pilB Acinetobacter baumannii D1279779

51.562

100

0.519
  pilB Legionella pneumophila strain ERS1305867

49.738

100

0.498
  pilB Vibrio cholerae strain A1552

51.589

93.531

0.483
  pilB Vibrio parahaemolyticus RIMD 2210633

47.331

98.252

0.465
  pilB Vibrio campbellii strain DS40M4

46.643

98.951

0.462
  pilF Neisseria gonorrhoeae MS11

44.84

98.252

0.441
  pilF Thermus thermophilus HB27

39.81

91.783

0.365

Multiple sequence alignment