Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   PG2T_RS12785 Genome accession   NZ_CP014671
Coordinates   2690048..2690887 (-) Length   279 a.a.
NCBI ID   WP_418268541.1    Uniprot ID   -
Organism   Immundisolibacter cernigliae strain TR3.2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2685048..2695887
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PG2T_RS12760 (PG2T_12775) - 2685117..2686490 (+) 1374 WP_068806215.1 TolC family outer membrane protein -
  PG2T_RS12765 (PG2T_12780) waaA 2686491..2687747 (-) 1257 WP_236953253.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  PG2T_RS12770 (PG2T_12785) - 2687859..2688521 (+) 663 WP_145931090.1 toluene tolerance protein -
  PG2T_RS15605 - 2688467..2688700 (-) 234 WP_083214921.1 DNA gyrase inhibitor YacG -
  PG2T_RS12775 (PG2T_12790) zapD 2688637..2689410 (-) 774 WP_068806223.1 cell division protein ZapD -
  PG2T_RS12780 (PG2T_12795) coaE 2689446..2690048 (-) 603 WP_068806226.1 dephospho-CoA kinase -
  PG2T_RS12785 (PG2T_12800) pilD 2690048..2690887 (-) 840 WP_418268541.1 prepilin peptidase Machinery gene
  PG2T_RS12790 (PG2T_12805) pilC 2690899..2692134 (-) 1236 WP_068806232.1 type II secretion system F family protein Machinery gene
  PG2T_RS12795 (PG2T_12810) pilB 2692143..2693861 (-) 1719 WP_068808361.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PG2T_RS12800 (PG2T_12815) - 2694185..2695174 (+) 990 WP_145931092.1 hypothetical protein -

Sequence

Protein


Download         Length: 279 a.a.        Molecular weight: 29788.52 Da        Isoelectric Point: 8.8073

>NTDB_id=173196 PG2T_RS12785 WP_418268541.1 2690048..2690887(-) (pilD) [Immundisolibacter cernigliae strain TR3.2]
MGTLLGSPALFAGVCLLLGLLVGSFLNVVVHRLPVMLQRQWQVDCGGAAAEGRYDLLAPASHCPHCKAPIAWRHNIPLLG
WLWLRGRCAACKAPISARYPLLELTAGVLAAAVAVRFGPGITAVAAIGLSLTLLTLTLIDIDHMLLPDAITLPGIWAGLL
VNLGGTFTDLRSAVIGAVAGYLVLWLVYHLFKWLTGKEGMGYGDFKLLALLGAWLGWQALPACILLASLVGTVYGLGAIL
HQGRNRQQPFPFGPFLAVAGWINLMWGEALRAAYWQAAL

Nucleotide


Download         Length: 840 bp        

>NTDB_id=173196 PG2T_RS12785 WP_418268541.1 2690048..2690887(-) (pilD) [Immundisolibacter cernigliae strain TR3.2]
CTGGGCACACTGCTGGGTAGCCCGGCGCTGTTTGCCGGTGTTTGTCTGCTGCTTGGGTTGCTGGTCGGCAGTTTCCTGAA
CGTGGTCGTGCATCGCCTGCCGGTGATGCTGCAGCGACAGTGGCAGGTCGATTGCGGCGGCGCGGCGGCAGAAGGCCGCT
ACGACCTGCTCGCGCCAGCCTCGCACTGCCCACACTGCAAGGCGCCGATTGCCTGGCGCCACAACATCCCCCTGCTGGGC
TGGCTGTGGCTGCGCGGTCGCTGCGCCGCCTGCAAGGCACCGATATCGGCCCGTTATCCGCTGCTGGAGCTGACCGCCGG
TGTGCTGGCTGCCGCGGTAGCCGTGCGCTTTGGACCCGGCATCACCGCCGTGGCGGCAATCGGCCTGAGCCTGACGCTGC
TGACACTCACCCTGATCGATATCGACCACATGCTGCTGCCGGATGCGATCACGCTGCCGGGCATCTGGGCCGGTTTGCTG
GTGAACCTGGGCGGCACCTTCACGGACCTGCGCTCGGCGGTCATCGGCGCGGTGGCCGGCTATCTTGTCCTGTGGCTGGT
GTATCACCTGTTCAAGTGGCTGACCGGCAAGGAAGGCATGGGTTACGGCGACTTCAAGCTGCTGGCGCTGCTGGGCGCCT
GGCTCGGCTGGCAGGCGCTGCCGGCGTGCATCCTGCTCGCCTCCCTGGTCGGAACCGTCTACGGCCTTGGCGCCATCCTG
CACCAGGGCCGGAATCGGCAGCAGCCGTTCCCGTTCGGGCCATTTCTGGCCGTCGCCGGCTGGATCAACCTGATGTGGGG
CGAAGCGCTGCGCGCGGCGTACTGGCAGGCGGCGCTGTGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(17-117)

Type IV leader peptidase family (Peptidase_A24)

(129-236)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

51.799

99.642

0.516
  pilD Vibrio campbellii strain DS40M4

47.5

100

0.477
  pilD Neisseria gonorrhoeae MS11

48.315

95.699

0.462
  pilD Acinetobacter baumannii D1279779

46.792

94.982

0.444
  pilD Acinetobacter nosocomialis M2

46.792

94.982

0.444

Multiple sequence alignment