Detailed information NTG0171854

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: AYJ52_RS09850
• Genome accession: NZ_CP014477
• Coordinates: 2109128..2110246 (-)
• Length: 372 a.a.
• NCBI ID: WP_017386714.1
• Uniprot ID: A0A009I1Y6
• Organism: Acinetobacter pittii strain AP_882
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2104128..2115246

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AYJ52_RS09820 (AYJ52_09820)	-	2104956..2105804 (+)	849	WP_017386716.1	class II glutamine amidotransferase	-
AYJ52_RS09825 (AYJ52_09825)	-	2106056..2106511 (+)	456	WP_002121698.1	bacteriohemerythrin	-
AYJ52_RS09830 (AYJ52_09830)	-	2106665..2107732 (+)	1068	WP_029769005.1	membrane protein	-
AYJ52_RS09835 (AYJ52_09835)	-	2107732..2108046 (+)	315	WP_014205982.1	RnfH family protein	-
AYJ52_RS09840 (AYJ52_09840)	omlA	2108111..2108509 (-)	399	WP_002121740.1	outer membrane protein assembly factor BamE	-
AYJ52_RS09845 (AYJ52_09845)	fur	2108621..2109058 (+)	438	WP_002121758.1	ferric iron uptake transcriptional regulator	-
AYJ52_RS09850 (AYJ52_09850)	pilU	2109128..2110246 (-)	1119	WP_017386714.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
AYJ52_RS09855 (AYJ52_09855)	pilT	2110266..2111303 (-)	1038	WP_002121749.1	type IV pilus twitching motility protein PilT	Machinery gene
AYJ52_RS09860 (AYJ52_09860)	yggS	2111430..2112122 (+)	693	WP_017386713.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41917.12 Da
• Isoelectric Point: 6.6404

>NTDB_id=171854 AYJ52_RS09850 WP_017386714.1 2109128..2110246(-) (pilU) [Acinetobacter pittii strain AP_882]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEIIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKSA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISYRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEVALKNTLRQAPDVILIGEIRTREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKSGQITYKDALKHADSPNDLRLTIKLAEEGPEQIAGTNQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=171854 AYJ52_RS09850 WP_017386714.1 2109128..2110246(-) (pilU) [Acinetobacter pittii strain AP_882]
ATGGATTTTAATGACCTGCTCAACCTGATGGTTGAAAAAAAATCATCCGACCTCTTTATTACAGATGGCGTTGCACCATC
GATGAAGATTAACGGGCAAATTGTTCCCATTTCAAAAAATAGTCTTTCAGGCGAAATTATCGGGCAACTATTACACTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAGACTCGTGAATGTAACTTTGCGATTATGAACCGTGATAAAAGTGCG
CGCTTTCGTGTAAGTGCTTTTCAGCAACGCGACATGCCAGGCATGGTACTACGTCGAATTGAAACCAAAATTCCTTCAAT
TGACGACTTACAATTACCGCCAGTGCTTAAAGATTTATCGATGACCAAACGTGGCATCATTATTTTTGTAGGGGCAACAG
GTACAGGTAAATCGACATCATTGGCTTCAATGATTAGCTATCGTAACCATAACTCTAAAGGTCACATCATTACCATTGAA
GACCCTATCGAGTTTATTCATGAACATGCTGGCTGCATCATTACCCAACGTGAAGTCGGGATCGATACTGATTCATTTGA
AGTAGCTTTAAAGAATACTTTGCGACAAGCACCCGATGTAATCTTGATTGGTGAGATCCGTACGCGTGAAGTCATGGACT
ATGCGATTGGTTTTGCCGAAACGGGTCACTTGGTCTTAGCCACGATGCATGCGAACAACGCCAACCAAGCACTTGACCGT
ATTATTCACTTCTTTGAAAGTGACCGTCATAGCCAGCTCTACATGGATTTATCGCTTAATTTAAAGGCCATGATTGCACA
ACAACTTATTCCGACTCCAGATGGTAATTCGCGCCGTGCAGCGATTGAGATTCTCATTAACTCACCACTAATTTCAGACT
ATATTCGCAAAGGCGAAATTCATGAAATTAAAGATTTAATGAAACGCTCACGTGAACTCGGTATGCAAACTTTTGACCAA
GCACTGTTTGATCTCTATAAGTCAGGACAAATTACCTACAAAGATGCATTAAAACATGCCGACTCACCAAACGATTTACG
TTTAACAATTAAGCTTGCCGAAGAAGGTCCTGAGCAAATTGCAGGCACAAATCAGCATTTAACCTTCGACAGACAGTAA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (32-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.132	100	0.763
pilU	Pseudomonas stutzeri DSM 10701	69.272	99.731	0.691
pilU	Vibrio cholerae strain A1552	54.054	99.462	0.538
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.934	97.849	0.401
pilT	Legionella pneumophila strain Lp02	39.941	90.86	0.363
pilT	Legionella pneumophila strain ERS1305867	39.941	90.86	0.363