Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   VN23_RS15360 Genome accession   NZ_CP014222
Coordinates   3413764..3415473 (+) Length   569 a.a.
NCBI ID   WP_046351590.1    Uniprot ID   -
Organism   Janthinobacterium sp. B9-8     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3408764..3420473
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VN23_RS15335 (VN23_15335) rimM 3409055..3409582 (-) 528 WP_046351594.1 ribosome maturation factor RimM -
  VN23_RS15340 (VN23_15340) rpsP 3409597..3409857 (-) 261 WP_046351593.1 30S ribosomal protein S16 -
  VN23_RS15345 (VN23_15345) ffh 3410098..3411450 (-) 1353 WP_046351592.1 signal recognition particle protein -
  VN23_RS15350 (VN23_15350) ccsA 3411506..3412354 (+) 849 WP_062654917.1 cytochrome c biogenesis protein CcsA -
  VN23_RS15355 (VN23_15355) - 3412400..3413662 (+) 1263 WP_046351591.1 HlyC/CorC family transporter -
  VN23_RS15360 (VN23_15360) pilB 3413764..3415473 (+) 1710 WP_046351590.1 type IV-A pilus assembly ATPase PilB Machinery gene
  VN23_RS15365 (VN23_15365) pilC 3415492..3416727 (+) 1236 WP_046351589.1 type II secretion system F family protein Machinery gene
  VN23_RS15370 (VN23_15370) pilD 3416727..3417596 (+) 870 WP_046351588.1 A24 family peptidase Machinery gene
  VN23_RS15375 (VN23_15375) coaE 3417596..3418222 (+) 627 WP_231743277.1 dephospho-CoA kinase -
  VN23_RS15380 (VN23_15380) zapD 3418283..3419041 (+) 759 WP_046351587.1 cell division protein ZapD -
  VN23_RS15385 (VN23_15385) - 3419038..3419229 (+) 192 WP_046351586.1 DNA gyrase inhibitor YacG -
  VN23_RS15390 (VN23_15390) - 3419320..3420063 (+) 744 WP_046351585.1 GNAT family N-acyltransferase -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62126.69 Da        Isoelectric Point: 5.2932

>NTDB_id=169551 VN23_RS15360 WP_046351590.1 3413764..3415473(+) (pilB) [Janthinobacterium sp. B9-8]
MSAPNSPVSGLARLLVQHGRLIEADAEALQSTANSSKTPFIEQLIRSRKMTARDVAEFSSQAFGYPLLDLDHIDPSYIPQ
GVLDNKVMLIQRIVPLFKRGTKLFIGLSDITNLQALEEVRFQTGLQVQPLVVEDSKLVSLLEKIVESTGANLSSMALDDV
DLDMGGGEEETSQNEAAALEVDDAPIVKYLQKILLDAINAGVSDIHFEPYEKFFRIRYRLDGVLREVAQPPLAIKDKLAS
RIKVISKLDISEKRVPQDGRMKLVLSKSRAIDFRVSTMPTMHGEKICMRILDPSSATLGIDALGYDPDQKEILLEAIQRP
YGMILVTGPTGSGKTVSLYTCLNILNQPGINISTAEDPCEINLPGVNQVNVNEKSGLTFAVALKAFLRQDPDIIMVGEIR
DLGTADISIKAAQTGHMVFSTLHTNDAPTTLTRMLNMGIAPFNIASSVILITAQRLARKLCSCKAPIDIPHQALLDAGFA
EEDLDGTWSPYKPVGCDICKGSGYKGRVGIYQVMPITDAMNRIIMTNGNAIDIADQAKRDGVRDLRGSGLRKVKQGLTSL
EEIMAVTNE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=169551 VN23_RS15360 WP_046351590.1 3413764..3415473(+) (pilB) [Janthinobacterium sp. B9-8]
ATGTCAGCACCTAACTCACCTGTATCTGGTCTTGCCCGCCTGCTTGTTCAGCACGGTAGATTAATTGAAGCAGACGCTGA
AGCTTTACAATCTACGGCAAACAGCAGCAAAACGCCCTTTATTGAGCAACTCATTCGCAGTCGTAAAATGACTGCGAGGG
ACGTTGCTGAATTCTCGTCGCAAGCGTTTGGCTACCCCCTGCTCGATCTTGACCATATTGATCCAAGCTATATACCTCAA
GGGGTTTTGGACAATAAAGTAATGTTGATACAGCGCATCGTGCCGCTCTTTAAACGGGGCACGAAGCTGTTCATCGGTCT
TTCAGACATCACTAATTTGCAGGCATTGGAAGAAGTTCGCTTTCAAACCGGCCTGCAAGTCCAGCCTCTGGTCGTCGAAG
ACAGCAAGCTTGTTTCCTTGCTTGAAAAAATCGTCGAATCCACAGGAGCCAACTTAAGCAGTATGGCGCTGGATGATGTT
GATTTAGACATGGGTGGTGGCGAGGAAGAAACAAGCCAGAATGAAGCTGCGGCCCTAGAGGTCGATGACGCGCCCATCGT
TAAATACCTGCAAAAGATTCTGCTCGACGCCATTAATGCAGGTGTTTCCGATATTCACTTTGAGCCTTACGAGAAATTTT
TCCGTATCCGCTACCGCCTTGATGGTGTGCTACGAGAAGTAGCACAACCTCCGCTGGCGATCAAAGACAAGCTCGCCTCA
CGCATTAAAGTTATTTCCAAGCTCGATATTTCTGAGAAGCGCGTTCCACAAGATGGCCGTATGAAATTGGTTTTGTCCAA
AAGCCGGGCCATCGACTTCCGAGTTTCCACCATGCCAACCATGCATGGCGAGAAAATTTGTATGCGGATTTTAGATCCTT
CCTCAGCCACCTTGGGGATTGATGCGCTGGGTTACGATCCAGACCAAAAAGAAATCTTGCTGGAAGCCATCCAGCGGCCG
TATGGCATGATTTTGGTTACAGGACCAACCGGCTCAGGAAAAACGGTTTCTCTTTACACTTGCTTGAACATCCTGAACCA
GCCGGGGATTAATATCTCTACCGCTGAAGATCCTTGTGAAATTAACTTGCCGGGTGTTAATCAGGTTAATGTAAACGAAA
AATCCGGGCTTACTTTTGCTGTCGCTCTGAAAGCTTTCTTACGCCAGGATCCCGACATCATCATGGTGGGGGAGATTCGC
GACCTAGGAACGGCAGATATTTCCATTAAAGCCGCGCAAACAGGGCATATGGTGTTCTCTACCCTGCACACCAATGATGC
ACCCACCACGCTCACACGGATGCTCAATATGGGAATTGCTCCTTTTAATATTGCGTCATCCGTTATTTTAATTACCGCAC
AACGCTTAGCCAGAAAGCTGTGTAGCTGCAAAGCGCCGATTGATATTCCTCATCAGGCCTTACTGGACGCAGGTTTTGCA
GAAGAAGATCTTGATGGCACATGGAGCCCTTACAAGCCCGTTGGCTGCGATATCTGCAAGGGTTCTGGCTATAAAGGTCG
GGTCGGTATTTACCAAGTGATGCCAATTACCGATGCAATGAACCGCATTATTATGACCAACGGTAATGCAATTGATATTG
CAGACCAAGCAAAACGCGATGGCGTACGTGATTTACGAGGCTCTGGCTTACGTAAAGTAAAACAAGGGCTAACCTCATTA
GAAGAGATCATGGCTGTTACCAATGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.052

100

0.555
  pilB Acinetobacter baumannii D1279779

55.575

99.297

0.552
  pilF Neisseria gonorrhoeae MS11

53.369

99.121

0.529
  pilB Legionella pneumophila strain ERS1305867

52.113

99.824

0.52
  pilB Vibrio cholerae strain A1552

46.679

97.891

0.457
  pilB Vibrio parahaemolyticus RIMD 2210633

45.406

99.473

0.452
  pilB Vibrio campbellii strain DS40M4

44.681

99.121

0.443
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.474

93.497

0.369
  pilB/pilB1 Synechocystis sp. PCC 6803

37.681

97.012

0.366
  pilF Thermus thermophilus HB27

36.814

99.297

0.366

Multiple sequence alignment