Detailed information NTG0169553

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: VN23_RS15370
• Genome accession: NZ_CP014222
• Coordinates: 3416727..3417596 (+)
• Length: 289 a.a.
• NCBI ID: WP_046351588.1
• Uniprot ID: A0A109RW61
• Organism: Janthinobacterium sp. B9-8
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3411727..3422596

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VN23_RS15355 (VN23_15355)	-	3412400..3413662 (+)	1263	WP_046351591.1	HlyC/CorC family transporter	-
VN23_RS15360 (VN23_15360)	pilB	3413764..3415473 (+)	1710	WP_046351590.1	type IV-A pilus assembly ATPase PilB	Machinery gene
VN23_RS15365 (VN23_15365)	pilC	3415492..3416727 (+)	1236	WP_046351589.1	type II secretion system F family protein	Machinery gene
VN23_RS15370 (VN23_15370)	pilD	3416727..3417596 (+)	870	WP_046351588.1	A24 family peptidase	Machinery gene
VN23_RS15375 (VN23_15375)	coaE	3417596..3418222 (+)	627	WP_231743277.1	dephospho-CoA kinase	-
VN23_RS15380 (VN23_15380)	zapD	3418283..3419041 (+)	759	WP_046351587.1	cell division protein ZapD	-
VN23_RS15385 (VN23_15385)	-	3419038..3419229 (+)	192	WP_046351586.1	DNA gyrase inhibitor YacG	-
VN23_RS15390 (VN23_15390)	-	3419320..3420063 (+)	744	WP_046351585.1	GNAT family N-acyltransferase	-
VN23_RS15395 (VN23_15395)	-	3420149..3420922 (+)	774	WP_046351584.1	lysophospholipid acyltransferase family protein	-
VN23_RS15400 (VN23_15400)	-	3420904..3421725 (-)	822	WP_046351583.1	symmetrical bis(5'-nucleosyl)-tetraphosphatase	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 31074.28 Da
• Isoelectric Point: 7.6148

>NTDB_id=169553 VN23_RS15370 WP_046351588.1 3416727..3417596(+) (pilD) [Janthinobacterium sp. B9-8]
MIELFLDSPAIFGGFLFVLGLLVGSFLNVVIHRIPKMMEHQFRQECQLIDAPIDAITPPSTTYNLVVPRSACPSCGHAIG
ALENIPVISYLVLRGRCKACKTQISPRYPMVELLCATLTTIAGLHFGVTIAAIAAIMLTWALIALVFIDADTYLLPDSIT
LPLLWLGLLFNLNNLFVPLDQAVIGAVAGYLVLWAVYWLFKLVTGKEGMGYGDFKLLAALGAWFGWQALPSIILLSSISG
AVIGIALTILAKRGMGKPMPFGPYLGIAGWLTLLIGGSLQQLLFSGVAA

Nucleotide

• Length: 870 bp

>NTDB_id=169553 VN23_RS15370 WP_046351588.1 3416727..3417596(+) (pilD) [Janthinobacterium sp. B9-8]
ATGATTGAGTTGTTTTTGGATAGCCCCGCCATATTTGGCGGTTTTTTATTCGTATTGGGTTTATTAGTGGGCAGTTTTTT
AAATGTCGTTATTCACCGTATCCCCAAAATGATGGAGCATCAATTCAGGCAAGAATGTCAGCTTATTGATGCCCCAATCG
ATGCCATTACTCCACCAAGCACCACCTACAACTTAGTCGTTCCACGCTCAGCCTGCCCAAGCTGCGGCCATGCAATTGGG
GCACTAGAGAATATCCCGGTGATCAGTTACTTGGTTTTACGAGGACGTTGCAAAGCATGTAAAACGCAGATCAGCCCTCG
CTATCCGATGGTTGAGCTGCTTTGCGCAACACTCACAACCATTGCAGGCTTACATTTTGGCGTTACAATCGCCGCAATCG
CCGCAATCATGCTGACATGGGCTTTAATTGCACTGGTCTTTATTGATGCAGATACTTATCTATTACCCGATAGCATCACA
CTCCCGCTGCTCTGGCTTGGCCTGCTCTTTAACTTAAATAACCTATTTGTTCCCCTCGACCAAGCTGTCATCGGTGCAGT
TGCCGGCTATTTAGTGCTGTGGGCTGTTTACTGGCTATTTAAGCTTGTCACTGGCAAAGAGGGCATGGGCTACGGCGACT
TTAAGCTACTTGCAGCATTGGGCGCTTGGTTTGGGTGGCAAGCTTTGCCAAGCATTATTTTGCTATCCTCAATCAGTGGC
GCAGTAATCGGTATTGCTCTTACCATACTTGCTAAGCGTGGAATGGGAAAACCCATGCCATTTGGGCCTTACCTTGGGAT
TGCAGGCTGGCTTACATTACTGATTGGCGGCTCGCTACAACAGCTTCTTTTTAGTGGTGTGGCGGCTTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (18-126)

• Type IV leader peptidase family (Peptidase_A24) (137-245)

3D structure

• AlphaFold DB: A0A109RW61

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	52.265	99.308	0.519
pilD	Vibrio cholerae strain A1552	51.93	98.616	0.512
pilD	Acinetobacter baumannii D1279779	50.929	93.08	0.474
pilD	Acinetobacter nosocomialis M2	50.186	93.08	0.467
pilD	Neisseria gonorrhoeae MS11	49.438	92.388	0.457