Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   AWU82_RS25485 Genome accession   NZ_CP014205
Coordinates   5570770..5572845 (+) Length   691 a.a.
NCBI ID   WP_064382838.1    Uniprot ID   -
Organism   Pseudomonas glycinae strain MS586     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5565770..5577845
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AWU82_RS25465 (AWU82_21870) comM 5567984..5569048 (+) 1065 WP_064382835.1 pilus assembly protein PilM Machinery gene
  AWU82_RS25470 (AWU82_21875) - 5569048..5569614 (+) 567 WP_064382836.1 PilN domain-containing protein -
  AWU82_RS25475 (AWU82_21880) pilO 5569611..5570234 (+) 624 WP_064382837.1 type 4a pilus biogenesis protein PilO -
  AWU82_RS25480 (AWU82_21885) - 5570231..5570755 (+) 525 WP_007952383.1 pilus assembly protein PilP -
  AWU82_RS25485 (AWU82_21890) pilQ 5570770..5572845 (+) 2076 WP_064382838.1 type IV pilus secretin PilQ Machinery gene
  AWU82_RS25490 (AWU82_21895) aroK 5572850..5573368 (+) 519 WP_011332081.1 shikimate kinase AroK -
  AWU82_RS25495 (AWU82_21900) aroB 5573521..5574621 (+) 1101 WP_011332082.1 3-dehydroquinate synthase -
  AWU82_RS25500 (AWU82_21905) - 5574632..5576239 (+) 1608 WP_064382839.1 AAA family ATPase -

Sequence

Protein


Download         Length: 691 a.a.        Molecular weight: 74995.08 Da        Isoelectric Point: 5.5724

>NTDB_id=169389 AWU82_RS25485 WP_064382838.1 5570770..5572845(+) (pilQ) [Pseudomonas glycinae strain MS586]
MNRIFSTLGFSLWIALLSPMVQAANLKALDVAALPGDRVELKLSFDGPPPQPKGYTTDSPARIALDLPGVANQLASKTRD
LGSGNARTATVVEANNRTRLIISLTQLAPYDTRVEGNTVFVVVGQGSKTAAPRTAARAPRAAPAAPVRTYSPTARAIRGV
DFQRGTAGEGNVVIDLSDPAIAPDIQEHDGKIILNFARTQLPEPLRVRLDVKDFATPVQFVNAGVSGDRATITVEPSGTF
DYSTYQTDNKLTVSIRPLTVDDLQKRNADRNSYNGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVP
WDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEAKV
DERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNTSGVSNG
SSTTIGTPGSTSTNSPFVDMGTVNNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQ
EASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSNT
QSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2076 bp        

>NTDB_id=169389 AWU82_RS25485 WP_064382838.1 5570770..5572845(+) (pilQ) [Pseudomonas glycinae strain MS586]
ATGAACAGGATTTTCTCCACCCTCGGTTTCTCGCTATGGATAGCGCTGCTTTCGCCGATGGTACAGGCAGCCAATCTGAA
GGCGCTGGACGTCGCGGCACTGCCGGGCGACCGGGTCGAGTTGAAGCTGTCGTTCGACGGCCCGCCTCCGCAGCCCAAGG
GCTACACCACCGACTCGCCGGCACGGATCGCCCTCGATCTGCCGGGTGTTGCCAATCAGCTGGCGAGCAAGACCCGTGAC
CTGGGCAGCGGCAATGCCCGCACGGCCACGGTGGTCGAGGCCAATAACCGGACGCGGCTGATCATCAGCCTGACGCAACT
GGCGCCTTACGACACCCGGGTCGAAGGCAATACGGTGTTTGTGGTGGTCGGGCAGGGCAGCAAAACCGCTGCTCCGCGTA
CGGCGGCTCGTGCACCGCGTGCGGCACCGGCGGCGCCGGTCCGCACGTATTCGCCGACCGCCAGGGCCATTCGCGGCGTG
GATTTCCAGCGTGGCACGGCGGGTGAGGGCAATGTGGTCATCGACTTGTCCGACCCGGCTATCGCCCCGGACATTCAGGA
ACACGACGGCAAGATCATCCTCAATTTCGCGCGTACCCAATTGCCCGAGCCGCTGCGGGTGCGCCTCGACGTCAAGGATT
TCGCCACCCCGGTGCAGTTCGTCAATGCCGGTGTCAGCGGTGATCGGGCGACGATCACCGTCGAACCCAGCGGTACGTTC
GACTATTCCACTTACCAGACCGACAACAAGCTGACCGTCAGCATCCGGCCGTTGACCGTCGATGACCTGCAAAAGCGCAA
CGCCGACCGCAACAGCTATAACGGCGAAAAGCTCTCTCTCAACTTCCAGGACATCGATGTGCGTTCGGTGCTGCAACTGA
TCGCCGACTTCACCAACCTCAATCTGGTGGCCAGCGATACGGTGCAGGGCGGGATCACCTTGCGCCTGCAAAACGTGCCG
TGGGATCAGGCGCTGGATCTGGTGCTCAAGACCAAAGGCCTGGACAAGCGCAAGGTCGGCAACGTGTTGCTGGTGGCGCC
GGCCGATGAAATCGCTGCGCGCGAGCGGCAGGAGCTGGAATCGCAGAAACAGATCGCCGAACTGGCGCCGTTGCGCCGCG
AGCTGTTGCAAGTGAACTACGCCAAGGCCGCGGACATCGCCAAGCTGTTCCAGTCGGTCACCAGCGCCGAGGCGAAGGTC
GACGAACGGGGTTCGATCACCGTCGATGAGCGGACCAACAACATCATTGCCTACCAGACCCAGGATCGCCTCGACGAACT
GCGGCGGATTGTGGCGCAGCTGGATATTCCGGTGCGTCAGGTGATGATCGAGGCGCGGATCGTCGAGGCCAACGTCGATT
ACGACAAGAGCCTGGGCGTGCGCTGGGGAGGTTCGATTCAGAACAAGGGCAACTGGAACACTTCCGGGGTCAGCAACGGT
TCGTCGACCACCATTGGCACGCCGGGCAGCACCAGCACCAACTCGCCGTTCGTCGACATGGGCACGGTCAACAACACGTC
CGGGATCGGCATCGCGTTCATCACCGACAACGTCTTGCTCGACCTTGAGCTGACCGCGATGGAGAAGACCGGCAACGGCG
AAATCGTCTCGCAGCCCAAGGTGGTCACCTCCGACAAGGAAACCGCGAAGATCCTCAAGGGCACCGAGATTCCGTACCAG
GAAGCCAGCTCCAGCGGCGCTACGTCGGTGTCCTTCAAGGAGGCGTCGCTGTCGCTGGAGGTGACGCCGCAGATCACCCC
GGACAACCGCATCATCATGGAGGTCAAGGTTACCAAGGACGAACCGGACTACCTGAACAAAGTGCAGGATGTACCGCCGA
TCAAGAAAAACGAGGTCAATGCCAAGGTGCTGGTGAACGACGGCGAAACCATCGTGATCGGGGGCGTTTTCTCAAATACT
CAAAGCAAGGTTGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGTTTC
GGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(375-440)

Bacterial type II and III secretion system protein (Secretin)

(526-682)

AMIN domain (AMIN)

(37-121)

Secretin and TonB N terminus short domain (STN)

(301-347)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.067

100

0.747
  comQ Acinetobacter baylyi ADP1

40.981

100

0.411
  pilQ Acinetobacter baumannii D1279779

37.834

100

0.41
  pilQ Acinetobacter baumannii strain A118

37.834

100

0.41
  pilQ Legionella pneumophila strain ERS1305867

37.798

97.25

0.368
  pilQ Legionella pneumophila strain Lp02

37.685

97.54

0.368

Multiple sequence alignment