Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   AWU82_RS25465 Genome accession   NZ_CP014205
Coordinates   5567984..5569048 (+) Length   354 a.a.
NCBI ID   WP_064382835.1    Uniprot ID   -
Organism   Pseudomonas glycinae strain MS586     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5562984..5574048
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AWU82_RS25455 (AWU82_21860) - 5563785..5565053 (+) 1269 WP_007952395.1 malic enzyme-like NAD(P)-binding protein -
  AWU82_RS25460 (AWU82_21865) - 5565319..5567766 (-) 2448 WP_064382834.1 penicillin-binding protein 1A -
  AWU82_RS25465 (AWU82_21870) comM 5567984..5569048 (+) 1065 WP_064382835.1 pilus assembly protein PilM Machinery gene
  AWU82_RS25470 (AWU82_21875) - 5569048..5569614 (+) 567 WP_064382836.1 PilN domain-containing protein -
  AWU82_RS25475 (AWU82_21880) pilO 5569611..5570234 (+) 624 WP_064382837.1 type 4a pilus biogenesis protein PilO -
  AWU82_RS25480 (AWU82_21885) - 5570231..5570755 (+) 525 WP_007952383.1 pilus assembly protein PilP -
  AWU82_RS25485 (AWU82_21890) pilQ 5570770..5572845 (+) 2076 WP_064382838.1 type IV pilus secretin PilQ Machinery gene
  AWU82_RS25490 (AWU82_21895) aroK 5572850..5573368 (+) 519 WP_011332081.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38165.72 Da        Isoelectric Point: 4.7595

>NTDB_id=169388 AWU82_RS25465 WP_064382835.1 5567984..5569048(+) (comM) [Pseudomonas glycinae strain MS586]
MLGLFNKKANTLLGIDISSTSVKLLELSRQGERYRVEAYAVEPLPANAVVEKNIAELEGVGQALSRVLVKARTGLKTVAV
AVAGSAVITKVIEMDAGLSDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSPRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAKQLAASQERLTVAVVDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLTL
EQAGLAKKQGGLPDDYVSEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEERLNTPTQVANP
FSDMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=169388 AWU82_RS25465 WP_064382835.1 5567984..5569048(+) (comM) [Pseudomonas glycinae strain MS586]
GTGCTAGGACTCTTCAATAAAAAGGCCAATACGTTACTGGGGATCGACATCAGCTCCACGTCGGTGAAGCTACTGGAGCT
GAGCCGTCAGGGGGAGCGCTACCGGGTCGAGGCGTACGCGGTGGAGCCGTTGCCGGCGAACGCCGTGGTCGAAAAGAACA
TCGCCGAACTCGAGGGCGTCGGCCAGGCACTGTCCCGGGTGCTGGTCAAGGCGCGTACCGGGCTGAAAACCGTGGCGGTG
GCCGTGGCGGGCTCGGCGGTGATCACCAAGGTCATCGAGATGGACGCCGGGCTGTCCGACGACGAGCTGGAAAACCAGCT
CAAGATCGAAGCCGATCAATACATTCCCTATCCGCTGGACGAGGTCGCCATCGACTTCGAAGTCCAGGGCGTTTCGCCGC
GCAACCCCGAACGGGTCAACGTGCTGCTGGCCGCCTGTCGCAAGGAAAACGTCGAAGTCCGCGAAGCGGCGCTCGCTCTC
GCCGGGCTGACCGCGCGGGTGGTAGATGTAGAGGCGTATGCGCTGGAGCGCTCGTTCGGCCTGCTGGCCAAGCAACTGGC
GGCTTCCCAGGAGCGCCTGACGGTGGCCGTGGTCGACATCGGCGCCACCATGACCACCCTCAGCGTCCTGCACAACGGCC
GGATCATCTACACCCGCGAACAACTGTTCGGCGGTCGACAGCTGACCGAGGAAATCCAGCGCCGTTACGGCCTGACCCTT
GAGCAGGCGGGGCTCGCCAAGAAGCAGGGCGGCCTGCCGGACGACTACGTCAGTGAGGTGCTGCAGCCATTTCGCGAGGC
GCTGGTGCAGCAGGTGTCGCGCTCCCTGCAGTTCTTTTTCGCCTCCGGCCAGTACAACGCTGTCGACCACATTCTGCTGG
CCGGTGGCACGGCGTCGGTGCCCGGCCTCGACCGGCTGATCGAAGAGCGCCTGAACACCCCGACCCAGGTTGCCAACCCG
TTTTCCGACATGGCCTTGAGCAGCAAGGTCAACGCCGGGGCCCTGGCGAGCGATGCGCCGGCCCTGATGATTGCCTGCGG
GCTCGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

56.215

100

0.562
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

54.237

100

0.542
  pilM Legionella pneumophila strain ERS1305867

48.87

100

0.489

Multiple sequence alignment