Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PcP3B5_RS28160 Genome accession   NZ_CP014158
Coordinates   6455232..6457319 (-) Length   695 a.a.
NCBI ID   WP_024128058.1    Uniprot ID   -
Organism   Pseudomonas citronellolis strain P3B5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6450232..6462319
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PcP3B5_RS28145 (PcP3B5_57880) - 6451864..6453540 (-) 1677 WP_061562995.1 AAA family ATPase -
  PcP3B5_RS28150 (PcP3B5_57890) aroB 6453547..6454653 (-) 1107 WP_009613636.1 3-dehydroquinate synthase -
  PcP3B5_RS28155 (PcP3B5_57900) aroK 6454705..6455196 (-) 492 WP_043268468.1 shikimate kinase AroK -
  PcP3B5_RS28160 (PcP3B5_57910) pilQ 6455232..6457319 (-) 2088 WP_024128058.1 type IV pilus secretin PilQ Machinery gene
  PcP3B5_RS28165 (PcP3B5_57920) pilP 6457376..6457900 (-) 525 WP_009613643.1 type 4a pilus biogenesis lipoprotein PilP -
  PcP3B5_RS28170 (PcP3B5_57930) pilO 6457897..6458553 (-) 657 WP_009613645.1 type 4a pilus biogenesis protein PilO -
  PcP3B5_RS28175 (PcP3B5_57940) pilN 6458550..6459149 (-) 600 WP_009613648.1 type 4a pilus biogenesis protein PilN -
  PcP3B5_RS28180 (PcP3B5_57950) pilM 6459149..6460213 (-) 1065 WP_009613650.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 695 a.a.        Molecular weight: 74822.95 Da        Isoelectric Point: 5.2731

>NTDB_id=168940 PcP3B5_RS28160 WP_024128058.1 6455232..6457319(-) (pilQ) [Pseudomonas citronellolis strain P3B5]
MNRTMTRLGFALLAGLFAPSLLAANLQRVDVASLPGDRVELKLGFDEPVAAPRGYTIEQPARIALDLPGVQNKLGSKNRE
LGVGNARSLTVVEAKDRTRLIINLTTLVPYSTRAQGNDLIILVGQGGGAPAAAASSAPSAAPAYAAAPRPAQPKPYVPVG
KAIRNIDFQRGEQGEGNVVIDLSDPSVSPDIQEQGGKIRLDFPRTQLPDSLRVRLDVKDFATPVQFVNASAAGDKASIVI
EPSGLYDYLVFQTDNRMTVSIKPMTANEAEKRKKDSFAYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITL
RLQNVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELEAQKQIAELAPLRRELIQVNYAKASDIAKLFQSVT
GTSSGPGAQPAAGTDNRGSITVDDRTNAIIAYQTQDRLDELRRIVSQLDIPVRQVMIEARIVEASVDYTKELGVQWGGSL
NNRGNFNITGGANSTSNTFVDLGVSTASPTSGIGIGFVTDNTTLDLQLNAMEKTGNGEVVSQPKVVTSDKETAKILKGSE
IPYQEASSSGATSVSFKEAALSLEVTPQITPDNRIIMQVKVNKDEPDYQNALLGVPPIKKNEVNAKVLVNDGETIVIGGV
FSNTQTKTTSKVPFLGDVPVVGRLFKNDYVTDTKNELLVFLTPRIMNNQAIAIDR

Nucleotide


Download         Length: 2088 bp        

>NTDB_id=168940 PcP3B5_RS28160 WP_024128058.1 6455232..6457319(-) (pilQ) [Pseudomonas citronellolis strain P3B5]
ATGAACAGAACTATGACCCGCCTGGGCTTTGCCCTGTTGGCCGGCCTTTTCGCGCCGAGCCTGCTCGCCGCCAACCTGCA
GCGCGTCGACGTGGCCTCGCTGCCGGGGGATCGCGTGGAGCTCAAGCTGGGCTTCGACGAGCCGGTGGCCGCGCCGCGTG
GCTACACCATCGAGCAGCCGGCGCGCATCGCCCTGGACCTGCCGGGCGTGCAGAACAAGCTGGGCAGCAAGAACCGCGAG
CTGGGCGTGGGCAACGCGCGCAGCCTGACCGTGGTCGAGGCCAAGGACCGCACCCGCCTGATCATCAACCTGACCACCCT
GGTGCCGTATTCCACCCGCGCCCAGGGCAACGACCTGATCATCCTGGTCGGCCAGGGGGGCGGCGCGCCCGCCGCGGCGG
CCAGCTCCGCACCGAGCGCGGCCCCGGCCTATGCCGCGGCGCCTCGCCCGGCGCAGCCCAAGCCCTACGTGCCGGTGGGC
AAGGCGATCCGCAACATCGACTTCCAGCGCGGCGAGCAGGGCGAGGGCAACGTGGTCATCGATCTGTCCGACCCGAGCGT
CAGCCCGGACATCCAGGAGCAGGGCGGCAAGATCCGCCTGGACTTCCCGCGCACCCAGCTGCCCGACAGCCTGCGCGTGC
GCCTGGACGTGAAGGACTTCGCCACCCCGGTGCAGTTCGTCAACGCCTCGGCGGCCGGCGACAAGGCCAGCATCGTCATC
GAGCCCAGCGGCCTGTACGACTACCTGGTGTTCCAGACCGACAACCGCATGACCGTCAGCATCAAGCCGATGACCGCCAA
CGAGGCCGAGAAGCGCAAGAAGGACAGCTTCGCCTACACCGGCGAGAAGCTGTCGCTGAACTTCCAGGACATCGACGTGC
GCTCGGTGCTGCAGCTGATCGCCGACTTCACCGACCTCAACCTGGTGGCCTCGGACACCGTGCAGGGCAACATCACCCTG
CGCCTGCAGAACGTGCCCTGGGACCAGGCGCTGGACCTGGTGCTCAAGACCAAGGGCCTGGACAAGCGCAAGGTCGGCAA
CGTCCTGCTGGTGGCGCCGGCCGACGAGATCGCCGCGCGCGAGCGCCAGGAGCTGGAAGCGCAGAAGCAGATCGCCGAAC
TGGCGCCCCTGCGTCGCGAACTGATCCAGGTGAACTACGCCAAGGCTTCCGACATCGCCAAGCTGTTCCAGTCCGTGACC
GGCACCAGTTCCGGCCCGGGCGCCCAGCCTGCCGCCGGCACCGACAACCGTGGCTCCATCACCGTCGACGACCGCACCAA
CGCCATCATCGCCTACCAGACCCAGGACCGCCTGGACGAGCTGCGACGCATCGTCTCGCAGCTGGACATCCCGGTGCGCC
AGGTGATGATCGAGGCGCGCATCGTCGAGGCCAGCGTCGACTACACCAAGGAACTGGGGGTGCAGTGGGGTGGTTCGCTG
AATAATCGCGGCAACTTCAACATCACCGGTGGCGCCAACAGCACGAGCAATACCTTCGTCGATCTGGGCGTCAGCACCGC
GTCCCCGACCTCCGGCATCGGCATCGGCTTCGTCACCGACAACACCACCCTGGACCTGCAGCTCAACGCCATGGAGAAGA
CCGGCAACGGCGAAGTGGTGTCGCAGCCGAAGGTGGTCACCTCGGACAAGGAAACCGCGAAGATCCTCAAGGGCTCGGAG
ATTCCCTACCAGGAAGCCAGCTCCAGCGGCGCCACCTCGGTGTCCTTCAAGGAGGCCGCGCTGTCCCTGGAGGTCACTCC
GCAGATCACCCCGGACAACCGCATCATCATGCAGGTCAAGGTGAACAAGGACGAGCCCGACTACCAGAACGCGCTGCTCG
GCGTGCCGCCGATCAAGAAGAACGAGGTCAACGCCAAGGTGCTGGTCAACGACGGTGAAACCATCGTCATCGGCGGGGTG
TTCTCCAACACCCAGACCAAGACCACCTCCAAGGTCCCGTTCCTCGGCGACGTACCGGTCGTCGGCCGCCTGTTCAAGAA
CGACTATGTGACGGACACCAAGAACGAGCTTCTGGTTTTCCTCACTCCCCGGATCATGAATAATCAGGCCATTGCCATCG
ATCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(381-454)

Secretin and TonB N terminus short domain (STN)

(307-353)

Bacterial type II and III secretion system protein (Secretin)

(530-686)

AMIN domain (AMIN)

(40-121)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

77.178

100

0.803
  pilQ Acinetobacter baumannii D1279779

40.699

100

0.419
  pilQ Acinetobacter baumannii strain A118

40.699

100

0.419
  comQ Acinetobacter baylyi ADP1

41.463

100

0.416
  pilQ Legionella pneumophila strain Lp02

38.946

98.273

0.383
  pilQ Legionella pneumophila strain ERS1305867

38.799

98.273

0.381

Multiple sequence alignment