Detailed information NTG0168941

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: PcP3B5_RS28180
• Genome accession: NZ_CP014158
• Coordinates: 6459149..6460213 (-)
• Length: 354 a.a.
• NCBI ID: WP_009613650.1
• Uniprot ID: A0A1A5D9E0
• Organism: Pseudomonas citronellolis strain P3B5
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 6454149..6465213

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PcP3B5_RS28155 (PcP3B5_57900)	aroK	6454705..6455196 (-)	492	WP_043268468.1	shikimate kinase AroK	-
PcP3B5_RS28160 (PcP3B5_57910)	pilQ	6455232..6457319 (-)	2088	WP_024128058.1	type IV pilus secretin PilQ	Machinery gene
PcP3B5_RS28165 (PcP3B5_57920)	pilP	6457376..6457900 (-)	525	WP_009613643.1	type 4a pilus biogenesis lipoprotein PilP	-
PcP3B5_RS28170 (PcP3B5_57930)	pilO	6457897..6458553 (-)	657	WP_009613645.1	type 4a pilus biogenesis protein PilO	-
PcP3B5_RS28175 (PcP3B5_57940)	pilN	6458550..6459149 (-)	600	WP_009613648.1	type 4a pilus biogenesis protein PilN	-
PcP3B5_RS28180 (PcP3B5_57950)	pilM	6459149..6460213 (-)	1065	WP_009613650.1	pilus assembly protein PilM	Machinery gene
PcP3B5_RS28185 (PcP3B5_57960)	-	6460399..6462831 (+)	2433	WP_061562996.1	penicillin-binding protein 1A	-
PcP3B5_RS28190 (PcP3B5_57970)	-	6463335..6463826 (-)	492	WP_043268464.1	M48 family metallopeptidase	-
PcP3B5_RS28195 (PcP3B5_57980)	-	6463888..6464658 (-)	771	WP_061562997.1	class I SAM-dependent methyltransferase	-
PcP3B5_RS28200 (PcP3B5_57990)	-	6464701..6465123 (-)	423	WP_009613658.1	hypothetical protein	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37773.09 Da
• Isoelectric Point: 4.4526

>NTDB_id=168941 PcP3B5_RS28180 WP_009613650.1 6459149..6460213(-) (pilM) [Pseudomonas citronellolis strain P3B5]
MLGLFTKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNSVVEKNIAELEGVGQALSRVVAKARTGVKTAAV
AVAGSAVITKTIEMDGGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGVSARNAERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERAFGLLGDQLGGNADELTVAVVDIGATMTTLSVLHHGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKDAVVQQVSRSLQFFFAAGQFNDVDYILLAGGTASIPDLDRLIQQKIGTPTLVANP
FADMVLSNKVNAGALASDAPALMIACGLAMRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=168941 PcP3B5_RS28180 WP_009613650.1 6459149..6460213(-) (pilM) [Pseudomonas citronellolis strain P3B5]
GTGCTAGGGCTCTTCACTAAGAAAGCGAACACACTGCTGGGGATCGACATCAGTTCGACTTCGGTCAAACTCCTCGAGTT
GAGCCGTTCGGGCGGGCGCTACAAGGTAGAGGCCTACGCCGTAGAGCCGCTCCCGCCGAACTCGGTAGTCGAGAAGAACA
TCGCCGAACTGGAGGGGGTCGGCCAGGCGCTGTCGCGGGTGGTCGCCAAGGCGCGCACCGGCGTGAAGACGGCTGCCGTG
GCGGTCGCCGGCTCGGCGGTCATCACCAAGACCATCGAGATGGACGGCGGGCTTTCCGAAGACGAGCTGGAGAACCAGCT
CAAGATCGAAGCCGACCAGTACATTCCCTATCCCCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGCGTATCCGCGC
GCAACGCCGAGCGCGTCGACGTGCTGCTGGCGGCCTGCCGCAAGGAGAACGTCGAGGTGCGCGAGGCCGCGCTGGCCCTG
GCCGGGCTCACCGCCAAGGTGGTGGACGTCGAGGCCTACGCGCTGGAGCGCGCCTTCGGCCTGCTCGGCGACCAGTTGGG
CGGCAACGCCGACGAGCTGACCGTCGCGGTGGTCGACATCGGCGCCACCATGACCACCCTCAGCGTCTTGCACCACGGCC
GCACCATCTATACCCGCGAGCAACTGTTCGGCGGGCGCCAGCTCACCGAGGAGATCCAGCGCCGCTATGGCCTGTCGGTG
GAAGAAGCCGGCCTCGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTGCTGCAACCGTTCAAGGATGC
GGTGGTGCAGCAGGTCTCGCGTTCGCTGCAGTTCTTCTTCGCCGCGGGCCAGTTCAACGACGTCGACTACATCCTGCTCG
CCGGCGGCACGGCTTCCATTCCCGACCTGGACCGGCTCATCCAGCAGAAGATCGGCACCCCGACGCTGGTGGCCAACCCC
TTCGCCGACATGGTGCTGAGCAACAAGGTGAATGCCGGGGCGCTGGCCAGTGATGCGCCGGCGCTGATGATTGCCTGCGG
TCTGGCGATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A1A5D9E0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	57.345	100	0.573
comM	Acinetobacter nosocomialis M2	57.345	100	0.573
comM	Acinetobacter baylyi ADP1	53.39	100	0.534
pilM	Legionella pneumophila strain ERS1305867	46.045	100	0.46