Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BHW76_RS23415 Genome accession   NZ_CP017249
Coordinates   4527101..4528486 (+) Length   461 a.a.
NCBI ID   WP_001025202.1    Uniprot ID   Q8X975
Organism   Escherichia coli strain NADC 5570/86-24/6565 isolate mutant     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4522101..4533486
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BHW76_RS23385 (BHW76_23385) aroP 4522649..4524022 (+) 1374 WP_010904500.1 aromatic amino acid transporter AroP -
  BHW76_RS23390 (BHW76_23390) ampE 4524065..4524919 (-) 855 WP_000172008.1 beta-lactamase regulator AmpE -
  BHW76_RS23395 (BHW76_23395) ampD 4524916..4525467 (-) 552 WP_000923722.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BHW76_RS23400 (BHW76_23400) nadC 4525555..4526448 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  BHW76_RS23410 (BHW76_23410) ppdD 4526651..4527091 (+) 441 WP_000360900.1 prepilin peptidase-dependent pilin -
  BHW76_RS23415 (BHW76_23415) pilB 4527101..4528486 (+) 1386 WP_001025202.1 type II secretion system protein GspE Machinery gene
  BHW76_RS23420 (BHW76_23420) hofC 4528476..4529678 (+) 1203 WP_000157271.1 protein transport protein HofC -
  BHW76_RS23425 (BHW76_23425) guaC 4529713..4530756 (-) 1044 WP_001217330.1 GMP reductase -
  BHW76_RS32275 - 4530912..4530956 (-) 45 WP_120795372.1 protein YacM -
  BHW76_RS23430 (BHW76_23430) coaE 4530981..4531601 (+) 621 WP_001269520.1 dephospho-CoA kinase -
  BHW76_RS23435 (BHW76_23435) zapD 4531601..4532344 (+) 744 WP_001194728.1 cell division protein ZapD -
  BHW76_RS23440 (BHW76_23440) yacG 4532354..4532551 (+) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  BHW76_RS23450 (BHW76_23450) mutT 4532642..4533040 (-) 399 WP_000736023.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50562.35 Da        Isoelectric Point: 6.8050

>NTDB_id=167351 BHW76_RS23415 WP_001025202.1 4527101..4528486(+) (pilB) [Escherichia coli strain NADC 5570/86-24/6565 isolate mutant]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLAGFAHALQQPQGLVLVTGPTGSGKTVTLYSALQKLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPVNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=167351 BHW76_RS23415 WP_001025202.1 4527101..4528486(+) (pilB) [Escherichia coli strain NADC 5570/86-24/6565 isolate mutant]
ATGAATATTCCACAGCTCACGGCCCTGTGCCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAAGTGGTTCATGT
TGCGGTGGTCGATGCCCCCTCACATGAGTTGCTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAGCCAGGCACTGGATGTTAACACGCTGGGAATGCAGCCGTTACAACTGGCGGGCTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACCGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAAAGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCCGGACTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACGGCAGAAATTGCCATTAAAGCCGCGCAAACCGGTCACCTGGTGTTGTCTACCCTACACACTAATTCC
ACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGGGTCGCCCGCTGGATGCTATCATCGGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGCAAACTTTGCCCACATTGTCGCCGGCAGCAAGGGGAGCCCATCCACATTCCAGTCAATGTATGGC
CGTCGCCGCTGCCCCACTGGCAAGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGCACTGCCTTATTTGAA
GTTCTGCCCATAACACCGGTCATACGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCCCGACA
GGCGGGTATGCGAACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TATTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)

Type II/IV secretion system protein (T2SSE)

(88-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8X975

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

50

83.297

0.416
  pilB Glaesserella parasuis strain SC1401

41.253

100

0.414
  pilB Acinetobacter baylyi ADP1

40.385

100

0.41
  pilB Vibrio campbellii strain DS40M4

48.32

83.948

0.406
  pilB Vibrio cholerae strain A1552

46.465

85.9

0.399
  pilB Vibrio parahaemolyticus RIMD 2210633

46.632

83.731

0.39
  pilB Haemophilus influenzae 86-028NP

44.961

83.948

0.377
  pilB Acinetobacter baumannii D1279779

43.909

85.466

0.375
  pilB Haemophilus influenzae Rd KW20

44.444

83.948

0.373
  pilF Neisseria gonorrhoeae MS11

44.156

83.514

0.369
  pilF Thermus thermophilus HB27

40.831

88.72

0.362