Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AL480_RS13280 Genome accession   NZ_CP014014
Coordinates   2755885..2757618 (-) Length   577 a.a.
NCBI ID   WP_033833107.1    Uniprot ID   -
Organism   Stenotrophomonas maltophilia strain FDAARGOS_92     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2750885..2762618
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AL480_RS13265 (AL480_13265) sucC 2751184..2752353 (-) 1170 WP_004154430.1 ADP-forming succinate--CoA ligase subunit beta -
  AL480_RS13270 (AL480_13270) - 2752711..2754324 (+) 1614 WP_005414304.1 ATP-binding protein -
  AL480_RS13275 (AL480_13275) pilR 2754385..2755758 (+) 1374 WP_033833106.1 sigma-54 dependent transcriptional regulator Regulator
  AL480_RS13280 (AL480_13280) pilB 2755885..2757618 (-) 1734 WP_033833107.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AL480_RS13285 (AL480_13285) pilA/pilAI 2757841..2758248 (-) 408 WP_033833108.1 pilin Machinery gene
  AL480_RS13290 (AL480_13290) - 2758398..2758826 (-) 429 WP_033833109.1 pilin -
  AL480_RS13295 (AL480_13295) pilC 2759179..2760438 (+) 1260 WP_033833111.1 type II secretion system F family protein Machinery gene
  AL480_RS13300 (AL480_13300) - 2760446..2761309 (+) 864 WP_005410800.1 A24 family peptidase -
  AL480_RS13305 (AL480_13305) coaE 2761321..2761932 (+) 612 WP_033833112.1 dephospho-CoA kinase -
  AL480_RS13310 (AL480_13310) - 2762125..2762511 (-) 387 WP_104920827.1 hypothetical protein -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62331.28 Da        Isoelectric Point: 5.8733

>NTDB_id=167194 AL480_RS13280 WP_033833107.1 2755885..2757618(-) (pilB) [Stenotrophomonas maltophilia strain FDAARGOS_92]
MNAVTTANLVGITGLARRLVQDGALDEATARDAMAKAAAARQPLPTWFAQNKLVNASQLAAANAVEFGMPLFDVSTFDAS
QNAMSLVSEELLRKHNVLPLFKRGGKLFVGTSNPTHSLDEIKFHTNLVVEPILVDEDQIRRTLEQWHASHDTLGDALGGD
DDGMGNLDVAAGDEDLGATGGESGIDAKGDDTPVVKFVNKVLVDAIRKGASDIHFEPYEDDYRVRLRIDGLLKMVARAPV
KLNQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKL
FLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPD
IIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCGNCKRPSSLPE
HALLAEGFTQAQLDAGIELYEAVGCDECTEGYKGRTGVYQVMPMTDEIATIVLAGGNALQIAEAAQQIGVNDLRQSALKK
AAAGITSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=167194 AL480_RS13280 WP_033833107.1 2755885..2757618(-) (pilB) [Stenotrophomonas maltophilia strain FDAARGOS_92]
ATGAACGCTGTCACCACCGCCAATCTTGTCGGCATCACCGGCCTGGCCCGTCGCCTGGTGCAGGACGGGGCCCTGGACGA
GGCCACCGCCCGCGACGCCATGGCCAAGGCCGCCGCCGCGCGCCAGCCGCTGCCGACGTGGTTCGCGCAGAACAAGCTGG
TCAATGCCTCGCAGCTGGCCGCCGCCAATGCGGTCGAGTTCGGCATGCCGCTGTTCGACGTGTCCACCTTCGACGCCAGC
CAGAACGCGATGAGCCTGGTCAGCGAGGAGCTGCTGCGCAAGCACAACGTGCTGCCGCTGTTCAAGCGCGGTGGCAAGCT
GTTCGTCGGCACCAGCAATCCGACCCACTCGCTGGACGAGATCAAGTTCCACACCAACCTGGTGGTCGAGCCGATCCTGG
TCGACGAGGACCAGATCCGGCGCACGCTGGAACAGTGGCACGCCAGCCATGACACCCTCGGCGATGCCCTGGGTGGTGAT
GACGATGGCATGGGCAACCTGGACGTGGCGGCCGGCGACGAGGATCTCGGCGCCACCGGCGGCGAGTCCGGCATCGATGC
CAAGGGTGACGACACCCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTCGATGCGATCCGCAAGGGCGCCTCGGACATCC
ACTTCGAGCCCTACGAAGACGATTACCGCGTGCGCCTGCGCATCGACGGCCTGCTGAAGATGGTCGCGCGCGCACCGGTG
AAGTTGAACCAGCGCATCGCCGCGCGCCTGAAGGTGATGGCACAGCTGGATATCGCCGAGAAGCGCGTCCCGCAGGACGG
CCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTCAGCACGCTGCCGACCCTGTTCGGCGAGAAGG
TGGTGCTGCGTATCCTCGATGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGTTACGAGCCGGACCAGCAGAAGCTG
TTCCTGGATGCCATCCACAAGCCGTACGGCATGGTGCTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTGTCGCTGTA
TACCGCGCTGGGCATCCTCAATGACGAGACGCGCAACATCTCCACCGCCGAAGACCCGGTGGAAATCCGCCTGCCCGGCG
TCAACCAGGTACAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCACTGCGCTCGTTCCTGCGACAGGACCCGGAC
ATCATCATGGTCGGCGAAATCCGCGACCTGGAAACGGCGGAGATCGCGATCAAGGCCGCGCAGACCGGCCACATGGTGCT
GTCCACGCTGCATACCAACGATGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGTATCGCGCCCTACAACATCACCA
GCTCGGTGACGCTGGTGATCGCCCAGCGCCTGGCGCGCCGCCTGTGCGGCAACTGCAAGCGTCCGTCCAGCCTGCCCGAA
CATGCGCTGTTGGCCGAAGGCTTCACCCAGGCGCAGCTGGATGCGGGCATCGAGCTGTACGAGGCCGTGGGCTGCGACGA
ATGCACCGAGGGCTACAAGGGCCGGACCGGCGTCTACCAGGTCATGCCGATGACCGATGAGATCGCCACCATCGTGCTGG
CCGGTGGCAATGCGCTGCAGATTGCCGAGGCGGCGCAGCAGATCGGGGTGAATGACCTGCGCCAGTCGGCATTGAAGAAG
GCGGCGGCGGGCATTACCAGCCTGGCCGAGATCAACCGCGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-150)

Type II/IV secretion system protein (T2SSE)

(200-467)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.25

100

0.556
  pilB Acinetobacter baylyi ADP1

54.701

100

0.555
  pilB Legionella pneumophila strain ERS1305867

51.93

98.787

0.513
  pilF Neisseria gonorrhoeae MS11

48.772

98.787

0.482
  pilB Vibrio cholerae strain A1552

48.854

98.267

0.48
  pilB Vibrio parahaemolyticus RIMD 2210633

45.455

99.133

0.451
  pilB Vibrio campbellii strain DS40M4

45.518

98.614

0.449

Multiple sequence alignment