Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BHT24_RS00545 Genome accession   NZ_CP017220
Coordinates   119151..120536 (-) Length   461 a.a.
NCBI ID   WP_044863698.1    Uniprot ID   -
Organism   Escherichia coli strain FAM21845     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 114151..125536
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BHT24_RS00515 (BHT24_00510) mutT 114481..114870 (+) 390 WP_000736007.1 8-oxo-dGTP diphosphatase MutT -
  BHT24_RS00520 (BHT24_00520) yacG 115086..115283 (-) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  BHT24_RS00525 (BHT24_00525) zapD 115293..116036 (-) 744 WP_001194734.1 cell division protein ZapD -
  BHT24_RS00530 (BHT24_00530) coaE 116036..116656 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  BHT24_RS27950 - 116681..116725 (+) 45 WP_120795372.1 protein YacM -
  BHT24_RS00535 (BHT24_00535) guaC 116881..117924 (+) 1044 WP_001217338.1 GMP reductase -
  BHT24_RS00540 (BHT24_00540) hofC 117959..119161 (-) 1203 WP_000157266.1 protein transport protein HofC -
  BHT24_RS00545 (BHT24_00545) pilB 119151..120536 (-) 1386 WP_044863698.1 type II secretion system protein GspE Machinery gene
  BHT24_RS00550 (BHT24_00550) pilA 120546..120986 (-) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  BHT24_RS00560 (BHT24_00560) nadC 121189..122082 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  BHT24_RS00565 (BHT24_00565) ampD 122170..122721 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BHT24_RS00570 (BHT24_00570) ampE 122718..123572 (+) 855 WP_044863697.1 beta-lactamase regulator AmpE -
  BHT24_RS00575 (BHT24_00575) aroP 123615..124988 (-) 1374 WP_072146193.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50629.36 Da        Isoelectric Point: 6.6612

>NTDB_id=167018 BHT24_RS00545 WP_044863698.1 119151..120536(-) (pilB) [Escherichia coli strain FAM21845]
MNIPQLTALCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVGQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQKLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDIESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=167018 BHT24_RS00545 WP_044863698.1 119151..120536(-) (pilB) [Escherichia coli strain FAM21845]
ATGAATATTCCACAGCTCACTGCCCTGTGTCTGCGTTATCATGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTAGTCGATGCACCTTCGCATGAGCTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTAACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGGGTCAGGCACTGGATGTCAACACGCTTGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACCGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAAAGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCAGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCCGGACTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACAGCAGAGATCGCTATTAAAGCGGCGCAAACTGGTCACCTGGTGTTGTCTACCCTACACACTAATTCC
ACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGGGTCGCCCGCTGGATGCTATCATCGGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGCAAACTTTGCCCACATTGTCGCCGGCAGCAAGGGGAGCCCATCCACATTCCAGACAATGTATGGC
CATCGCCGCTGCCCCACTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGTACGGCCTTATTTGAA
GTTCTGCCCATAACGCCGGTCATTCGTCAGCTTATTTCCGCTAATACCGACATTGAATCGCTGGAAACGCACGCACGACA
GGCGGGTATGCGTACGCTTTTTGAAAACGGCTGCCTGGCCGTAGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TACTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)

Type II/IV secretion system protein (T2SSE)

(88-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

42.236

100

0.443
  pilB Legionella pneumophila strain ERS1305867

50

83.297

0.416
  pilB Glaesserella parasuis strain SC1401

41.253

100

0.414
  pilB Acinetobacter baylyi ADP1

39.957

100

0.406
  pilB Vibrio cholerae strain A1552

46.923

84.599

0.397
  pilB Vibrio parahaemolyticus RIMD 2210633

45.897

84.599

0.388
  pilB Haemophilus influenzae 86-028NP

45.22

83.948

0.38
  pilB Acinetobacter baumannii D1279779

43.655

85.466

0.373
  pilB Haemophilus influenzae Rd KW20

44.444

83.948

0.373
  pilF Neisseria gonorrhoeae MS11

44.156

83.514

0.369

Multiple sequence alignment