Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   BHT24_RS00550 Genome accession   NZ_CP017220
Coordinates   120546..120986 (-) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain FAM21845     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 115546..125986
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BHT24_RS00530 (BHT24_00530) coaE 116036..116656 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  BHT24_RS27950 - 116681..116725 (+) 45 WP_120795372.1 protein YacM -
  BHT24_RS00535 (BHT24_00535) guaC 116881..117924 (+) 1044 WP_001217338.1 GMP reductase -
  BHT24_RS00540 (BHT24_00540) hofC 117959..119161 (-) 1203 WP_000157266.1 protein transport protein HofC -
  BHT24_RS00545 (BHT24_00545) pilB 119151..120536 (-) 1386 WP_044863698.1 type II secretion system protein GspE Machinery gene
  BHT24_RS00550 (BHT24_00550) pilA 120546..120986 (-) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  BHT24_RS00560 (BHT24_00560) nadC 121189..122082 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  BHT24_RS00565 (BHT24_00565) ampD 122170..122721 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BHT24_RS00570 (BHT24_00570) ampE 122718..123572 (+) 855 WP_044863697.1 beta-lactamase regulator AmpE -
  BHT24_RS00575 (BHT24_00575) aroP 123615..124988 (-) 1374 WP_072146193.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=167019 BHT24_RS00550 WP_000360904.1 120546..120986(-) (pilA) [Escherichia coli strain FAM21845]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=167019 BHT24_RS00550 WP_000360904.1 120546..120986(-) (pilA) [Escherichia coli strain FAM21845]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363