Detailed information NTG0166499

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: AUL54_RS17905
• Genome accession: NZ_CP013950
• Coordinates: 3648783..3649397 (+)
• Length: 204 a.a.
• NCBI ID: WP_060964714.1
• Uniprot ID: A0AAI8HMV7
• Organism: Bacillus sp. SDLI1
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3643783..3654397

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AUL54_RS17870 (AUL54_17880)	aroE	3644471..3645313 (+)	843	WP_060964710.1	shikimate dehydrogenase	-
AUL54_RS17875 (AUL54_17885)	yhbY	3645307..3645597 (+)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
AUL54_RS17880 (AUL54_17890)	-	3645608..3646177 (+)	570	WP_016938889.1	nicotinate-nucleotide adenylyltransferase	-
AUL54_RS17885 (AUL54_17895)	yqeK	3646167..3646727 (+)	561	WP_060964711.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
AUL54_RS17890 (AUL54_17900)	rsfS	3646744..3647100 (+)	357	WP_016938891.1	ribosome silencing factor	-
AUL54_RS17895 (AUL54_17905)	-	3647097..3647834 (+)	738	WP_060964712.1	class I SAM-dependent methyltransferase	-
AUL54_RS17900 (AUL54_17910)	comER	3647903..3648724 (-)	822	WP_060964713.1	late competence protein ComER	-
AUL54_RS17905 (AUL54_17915)	comEA	3648783..3649397 (+)	615	WP_060964714.1	helix-hairpin-helix domain-containing protein	Machinery gene
AUL54_RS17910 (AUL54_17920)	-	3649463..3650032 (+)	570	WP_016938895.1	ComE operon protein 2	-
AUL54_RS17915 (AUL54_17925)	comEC	3650033..3652384 (+)	2352	WP_060964715.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
AUL54_RS20905	-	3652403..3652537 (-)	135	WP_003152870.1	YqzM family protein	-
AUL54_RS21280	-	3652578..3652732 (+)	155	Protein_3561	hypothetical protein	-
AUL54_RS17920 (AUL54_17930)	holA	3652772..3653813 (+)	1042	Protein_3562	DNA polymerase III subunit delta	-
AUL54_RS17925 (AUL54_17935)	rpsT	3653831..3654097 (-)	267	WP_003152876.1	30S ribosomal protein S20	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22272.36 Da
• Isoelectric Point: 4.7944

>NTDB_id=166499 AUL54_RS17905 WP_060964714.1 3648783..3649397(+) (comEA) [Bacillus sp. SDLI1]
MTKAMMTEQMRRNLKILGVCLAAGAVFICIWLFRPEKEEPVKQQSVLGQTASASGTDKTDTADDKIMIDMKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKEKADELNVNLAEQLQDGTVVYIPSEGEEENRPKTAAGEKEDAPVNINTATLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=166499 AUL54_RS17905 WP_060964714.1 3648783..3649397(+) (comEA) [Bacillus sp. SDLI1]
ATGACGAAAGCGATGATGACGGAGCAGATGCGGCGGAACCTGAAAATTCTGGGTGTTTGTCTTGCGGCAGGAGCGGTTTT
TATCTGCATTTGGCTGTTTCGTCCTGAAAAAGAAGAACCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGGCACGGACAAAACAGACACCGCAGACGATAAGATTATGATTGATATGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAGGCGGGAGACAGAGTGACACAAGCCATTGAAAAAGCCGGCGGCCTGAAGGAAAAGGCGGATGAACTGAATGT
TAATTTAGCAGAGCAGCTGCAAGACGGGACGGTCGTTTATATTCCGAGCGAAGGCGAAGAGGAGAATCGCCCGAAAACCG
CAGCAGGTGAGAAGGAGGATGCGCCTGTCAATATCAATACGGCAACTCTGGACGAACTTCAGGCGATTTCCGGTGTGGGG
CAGAAAAAAGCGGAAGCAATTATCGCCTACCGGGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGTTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (142-202)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.5	98.039	0.574
comEA	Lactococcus lactis subsp. cremoris KW2	36.715	100	0.373
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.232	100	0.368
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis SK321	35.749	100	0.363