Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   AT984_RS14790 Genome accession   NZ_CP013692
Coordinates   3412287..3413954 (+) Length   555 a.a.
NCBI ID   WP_082680045.1    Uniprot ID   -
Organism   Paucibacter sp. KCTC 42545     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3407287..3418954
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AT984_RS14765 (AT984_14765) - 3407299..3408111 (+) 813 WP_058720748.1 CheR family methyltransferase -
  AT984_RS14770 (AT984_14770) - 3408130..3408729 (+) 600 WP_231741431.1 chemotaxis protein CheD -
  AT984_RS14775 (AT984_14775) - 3408764..3409873 (+) 1110 WP_058720749.1 chemotaxis response regulator protein-glutamate methylesterase -
  AT984_RS14780 (AT984_14780) - 3409939..3410304 (-) 366 WP_058720750.1 response regulator -
  AT984_RS14785 (AT984_14785) - 3410503..3412047 (+) 1545 WP_197418115.1 methyl-accepting chemotaxis protein -
  AT984_RS14790 (AT984_14790) pilF 3412287..3413954 (+) 1668 WP_082680045.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AT984_RS14795 (AT984_14795) pilC 3413993..3415219 (+) 1227 WP_058720753.1 type II secretion system F family protein Machinery gene
  AT984_RS14800 (AT984_14800) - 3415228..3416157 (+) 930 WP_058720754.1 A24 family peptidase -
  AT984_RS14805 (AT984_14805) coaE 3416154..3416759 (+) 606 WP_058720755.1 dephospho-CoA kinase -
  AT984_RS14810 (AT984_14810) zapD 3416873..3417628 (+) 756 WP_058722348.1 cell division protein ZapD -
  AT984_RS14815 (AT984_14815) - 3417640..3417843 (+) 204 WP_058720756.1 DNA gyrase inhibitor YacG -
  AT984_RS14820 (AT984_14820) - 3417832..3418443 (-) 612 WP_058720757.1 HAD family phosphatase -

Sequence

Protein


Download         Length: 555 a.a.        Molecular weight: 60943.17 Da        Isoelectric Point: 5.7940

>NTDB_id=164102 AT984_RS14790 WP_082680045.1 3412287..3413954(+) (pilF) [Paucibacter sp. KCTC 42545]
MLVHAGKLPQKQAEELMRQAREKKTSFVNAVIAAGTLSSAELAHTLSTALSLPLLDISAMDVQRMPRDIVDTKLAQQYQI
VVLGRRGNRLFIGGADPTDQDVVERIKFATQLSPEWVIVEHDKLSRMLQGSAASASEALDQLAGGDFEFDITDEENAPPQ
EAAELADVEDAPVVRFLQKMLVDAINLRASDLHFEPYEFHYRVRFRVDGELREITQPPIAIKDKLASRIKVLSRLDIAER
RIPQDGRMKLKFGAKAIDFRISTLPTLFGEKIVIRILDPSSAKLGIDALGYEKIEKDRLLAAIARPYGMVLVTGPTGSGK
TVSLYTCLNILNQPGVNISTVEDPAEINLPGINQVNVNDKAGLGFSAALKSFLRQDPDIIMVGEIRDLETADIAIKAAQT
GHMVMSTLHTNDAPKTLSRLLNMGVAPFNIASSVLLITAQRLVRRLCENCKAPAEYPRDALLRAGFAEEELDGSWKQYRA
VGCANCTNGYRGRVGLYQVMPITEPIQRIILAEGSAMDIAVQAQQEGVRDLRQFGLVKVRAGVTTLEEVLAATNE

Nucleotide


Download         Length: 1668 bp        

>NTDB_id=164102 AT984_RS14790 WP_082680045.1 3412287..3413954(+) (pilF) [Paucibacter sp. KCTC 42545]
ATGCTGGTGCATGCGGGCAAGCTGCCGCAAAAGCAGGCCGAGGAGCTGATGCGGCAGGCTCGCGAGAAGAAGACCAGCTT
CGTCAATGCGGTCATCGCGGCGGGCACGCTCAGCTCGGCCGAGCTGGCGCACACCTTGTCAACAGCGCTGTCGCTGCCGC
TGCTGGACATCAGCGCCATGGATGTGCAGCGCATGCCGCGCGACATCGTCGACACCAAGCTAGCCCAGCAGTACCAGATC
GTGGTGCTGGGCCGACGCGGCAACCGGCTCTTCATCGGCGGCGCCGACCCGACCGATCAGGACGTGGTCGAGCGCATCAA
GTTCGCCACCCAACTCAGCCCCGAATGGGTGATCGTCGAGCACGACAAGCTCAGCCGCATGCTGCAGGGTTCGGCCGCCA
GCGCCAGCGAGGCGCTAGACCAACTCGCCGGCGGCGATTTTGAGTTCGACATCACCGACGAGGAAAACGCGCCCCCGCAA
GAAGCCGCCGAATTGGCCGATGTGGAAGACGCGCCGGTGGTGCGCTTCCTGCAGAAAATGTTGGTGGACGCCATCAATCT
GCGCGCCTCGGACTTGCATTTCGAGCCCTACGAGTTCCATTACCGCGTGCGCTTCCGGGTCGACGGAGAGCTGCGCGAAA
TCACCCAACCGCCCATTGCCATCAAGGACAAGCTGGCCTCGCGCATCAAGGTGCTGTCGCGCCTGGACATCGCCGAGCGC
CGCATCCCGCAAGATGGACGCATGAAGCTCAAGTTCGGCGCCAAGGCGATCGACTTCCGGATCTCCACCTTGCCGACCCT
GTTCGGCGAGAAGATCGTGATCCGTATTCTTGACCCGTCCTCCGCCAAGCTGGGCATTGACGCCCTGGGCTACGAGAAGA
TTGAGAAAGACCGCTTGCTGGCCGCCATCGCGCGGCCCTACGGCATGGTGCTGGTCACCGGCCCGACCGGTTCGGGCAAG
ACAGTGTCGCTGTACACCTGCTTGAACATCCTGAACCAGCCGGGCGTCAACATATCTACCGTCGAAGATCCGGCCGAAAT
CAACTTGCCTGGCATCAACCAGGTGAACGTGAATGACAAGGCCGGCCTGGGCTTCTCGGCAGCGCTGAAGTCCTTCCTGC
GCCAGGATCCCGACATCATCATGGTCGGTGAAATTCGCGACCTGGAGACAGCCGATATCGCCATCAAGGCCGCGCAAACC
GGCCATATGGTGATGTCCACCCTGCACACCAATGACGCACCCAAGACGCTCAGCCGTTTGCTGAATATGGGCGTGGCGCC
CTTCAACATCGCCTCCAGCGTCTTGCTGATCACGGCCCAGCGCCTGGTGCGGCGGCTGTGCGAAAACTGCAAGGCGCCGG
CCGAATATCCGCGCGACGCTTTACTGCGCGCCGGATTTGCCGAAGAAGAGTTGGATGGTTCGTGGAAGCAGTACCGCGCC
GTGGGCTGCGCCAATTGCACCAATGGCTACCGCGGTCGGGTTGGCCTCTACCAAGTCATGCCCATCACCGAGCCGATCCA
GCGCATCATCCTGGCCGAAGGCTCGGCCATGGACATCGCTGTACAAGCCCAGCAAGAAGGCGTGCGCGACTTGCGGCAGT
TCGGCTTGGTCAAGGTGCGCGCGGGCGTGACCACCCTGGAAGAAGTGCTTGCCGCAACAAATGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(32-133)

Type II/IV secretion system protein (T2SSE)

(179-445)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

53.546

100

0.544
  pilB Acinetobacter baumannii D1279779

53.532

96.937

0.519
  pilB Acinetobacter baylyi ADP1

51.439

100

0.515
  pilB Legionella pneumophila strain ERS1305867

50.899

100

0.51
  pilB Vibrio cholerae strain A1552

46.512

100

0.468
  pilB Vibrio parahaemolyticus RIMD 2210633

45.31

100

0.461
  pilB Vibrio campbellii strain DS40M4

45.617

100

0.459
  pilF Thermus thermophilus HB27

36.412

100

0.369
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.086

96.036

0.366

Multiple sequence alignment