Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CPter291_RS22240 Genome accession   NZ_CP013236
Coordinates   4969086..4970807 (+) Length   573 a.a.
NCBI ID   WP_062119196.1    Uniprot ID   -
Organism   Collimonas pratensis strain Ter291     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4964086..4975807
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CPter291_RS22225 (CPter291_4700) ispB 4966223..4967173 (+) 951 WP_156480244.1 octaprenyl diphosphate synthase -
  CPter291_RS22235 (CPter291_4702) - 4967516..4968796 (+) 1281 WP_062119193.1 HlyC/CorC family transporter -
  CPter291_RS22240 (CPter291_4704) pilB 4969086..4970807 (+) 1722 WP_062119196.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CPter291_RS22245 (CPter291_4705) pilC 4970823..4972043 (+) 1221 WP_062119198.1 type II secretion system F family protein Machinery gene
  CPter291_RS22250 (CPter291_4706) pilD 4972043..4972909 (+) 867 WP_062119201.1 prepilin peptidase Machinery gene
  CPter291_RS22255 (CPter291_4707) coaE 4972916..4973569 (+) 654 WP_062119204.1 dephospho-CoA kinase -
  CPter291_RS22260 (CPter291_4708) zapD 4973692..4974447 (+) 756 WP_061944425.1 cell division protein ZapD -
  CPter291_RS22265 (CPter291_4709) yacG 4974470..4974658 (+) 189 WP_061944428.1 DNA gyrase inhibitor YacG -
  CPter291_RS22270 (CPter291_4710) mutT 4974688..4975104 (-) 417 WP_062119207.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 62636.44 Da        Isoelectric Point: 5.1882

>NTDB_id=160951 CPter291_RS22240 WP_062119196.1 4969086..4970807(+) (pilB) [Collimonas pratensis strain Ter291]
MAAVFPNTSTTGPISGLARALLQAGRLSTVQMEALHKKASGDQTPFIAALLESGHLDAPALALFCAETFGYPQLDLSVLN
LSTLPEKAIDAKLMESHFVLPLAKRGNKIYVALSDPTNMQALDQIKFQTELMVEPIIVEHPVLVKQIQKLVKSAEQSLSE
MVGDDQEINFVEEDTAAIADATADIDDAPVVRFLQKILTDAINQGASDLHFEPFEKFYRIRFRVDGVLRDMAQPPLSIKE
KLASRIKVISKLDISEKRVPQDGRMRLVVSKTRAIDFRVSTLPTLFGEKIVMRILDPSQAQMGIDALGYDPDQKAILMDA
IQRPYGMVLVTGPTGSGKTVSLYTCLNILNQPGINISTAEDPAEINLPGVNQVNINDRAGLTFPVALKAFLRQDPDIIMV
GEIRDLETADISIKAAQTGHMVFSTLHTNDAPSTLTRLMNMGVPAFNIASSVILITAQRLARRLCSCKQPITIQEEALAE
AGFTEDDIDGSWLPYKAVGCERCNGTGYKGRVGIYQIMPITEEIERIILAHGTALEIEAQAKREGVKTLRQSGLMKVKQG
MTSLEEVLGCTNI

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=160951 CPter291_RS22240 WP_062119196.1 4969086..4970807(+) (pilB) [Collimonas pratensis strain Ter291]
ATGGCTGCAGTATTTCCTAACACCAGTACCACCGGTCCGATCTCGGGACTTGCCCGCGCATTGCTGCAAGCCGGCCGCTT
GTCGACGGTGCAGATGGAAGCCTTGCACAAGAAAGCCAGCGGCGATCAAACGCCGTTCATCGCCGCCTTGCTGGAGAGCG
GCCATCTCGATGCGCCCGCGCTGGCGCTGTTTTGCGCCGAGACCTTCGGTTATCCGCAGCTGGACCTGAGCGTGCTCAAC
CTGAGCACGCTGCCGGAAAAAGCCATCGACGCCAAGCTGATGGAAAGCCACTTCGTGCTGCCGCTGGCGAAGCGCGGCAA
CAAGATCTACGTGGCGCTGTCCGATCCGACCAACATGCAGGCGCTGGACCAGATCAAGTTCCAGACCGAGCTGATGGTCG
AACCGATCATTGTCGAGCATCCGGTGCTGGTGAAGCAGATCCAGAAGCTGGTCAAATCGGCCGAGCAAAGCCTGAGCGAA
ATGGTCGGCGACGACCAGGAAATCAATTTCGTCGAAGAGGATACGGCAGCGATTGCCGACGCCACCGCCGATATCGATGA
CGCGCCGGTAGTACGCTTCCTGCAAAAGATCCTGACCGATGCGATCAACCAGGGCGCGTCCGACTTGCATTTCGAACCGT
TTGAAAAATTCTACCGGATCCGTTTCCGGGTCGACGGTGTGCTGCGCGACATGGCGCAACCGCCGTTGTCGATCAAGGAA
AAACTGGCCTCCCGCATCAAGGTGATCTCGAAGCTGGATATTTCCGAAAAGCGGGTGCCGCAGGATGGCCGGATGCGGCT
GGTGGTGTCCAAGACACGCGCTATCGATTTCCGGGTCAGCACCCTGCCCACGCTGTTCGGTGAAAAAATCGTGATGCGTA
TCCTGGACCCAAGCCAGGCGCAGATGGGGATCGACGCGTTGGGCTACGACCCGGACCAGAAAGCGATCCTGATGGACGCC
ATCCAGCGTCCCTACGGCATGGTGCTGGTGACCGGCCCTACCGGCTCCGGTAAAACCGTGTCGCTCTATACCTGCCTGAA
TATCCTGAACCAGCCGGGCATCAATATCTCCACCGCCGAAGATCCGGCCGAAATCAATTTGCCCGGCGTCAATCAGGTCA
ACATCAACGACCGCGCCGGCCTGACTTTCCCGGTGGCGCTGAAGGCCTTCCTGCGGCAGGATCCCGACATCATCATGGTC
GGTGAAATCCGCGACCTGGAAACCGCCGACATCTCGATCAAGGCTGCGCAAACCGGGCACATGGTGTTTTCCACCCTGCA
TACCAACGATGCGCCGTCGACGCTGACGCGCTTGATGAACATGGGCGTGCCGGCTTTCAATATTGCATCTTCCGTGATCC
TGATCACCGCCCAGCGGCTGGCGCGCCGCTTGTGCAGCTGCAAGCAGCCGATTACGATCCAGGAGGAAGCCCTGGCTGAA
GCCGGCTTCACCGAAGACGACATCGACGGTAGCTGGCTGCCATACAAGGCCGTCGGCTGCGAACGCTGCAACGGCACCGG
CTACAAGGGACGGGTCGGCATCTATCAGATCATGCCGATCACAGAAGAAATAGAACGCATCATTCTGGCCCATGGCACGG
CGCTGGAAATCGAAGCACAAGCCAAGCGCGAAGGCGTCAAAACCCTGCGACAGTCGGGATTGATGAAGGTTAAACAAGGG
ATGACCAGCCTCGAAGAAGTGCTCGGTTGCACCAATATCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(196-463)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.026

98.953

0.544
  pilB Acinetobacter baylyi ADP1

54.021

99.825

0.539
  pilF Neisseria gonorrhoeae MS11

53.004

98.778

0.524
  pilB Legionella pneumophila strain ERS1305867

51.515

97.906

0.504
  pilB Vibrio parahaemolyticus RIMD 2210633

47.979

99.302

0.476
  pilB Vibrio cholerae strain A1552

47.425

98.255

0.466
  pilB Vibrio campbellii strain DS40M4

45.951

99.127

0.456
  pilF Thermus thermophilus HB27

40.183

95.113

0.382
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.454

92.321

0.373

Multiple sequence alignment