Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CFter6_RS21975 Genome accession   NZ_CP013232
Coordinates   5021305..5023029 (+) Length   574 a.a.
NCBI ID   WP_014007897.1    Uniprot ID   -
Organism   Collimonas fungivorans strain Ter6     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5016305..5028029
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CFter6_RS21960 (CFter6_4796) ispB 5018729..5019679 (+) 951 WP_150118924.1 octaprenyl diphosphate synthase -
  CFter6_RS21970 (CFter6_4798) - 5019892..5021181 (+) 1290 WP_014007896.1 HlyC/CorC family transporter -
  CFter6_RS21975 (CFter6_4800) pilB 5021305..5023029 (+) 1725 WP_014007897.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CFter6_RS21980 (CFter6_4801) pilC 5023046..5024278 (+) 1233 WP_061541721.1 type II secretion system F family protein Machinery gene
  CFter6_RS21985 (CFter6_4802) pilD 5024278..5025144 (+) 867 WP_061541722.1 prepilin peptidase Machinery gene
  CFter6_RS21990 (CFter6_4803) coaE 5025151..5025804 (+) 654 WP_061541723.1 dephospho-CoA kinase -
  CFter6_RS21995 (CFter6_4804) zapD 5025935..5026690 (+) 756 WP_014007901.1 cell division protein ZapD -
  CFter6_RS22000 (CFter6_4805) - 5026712..5026900 (+) 189 WP_061541724.1 DNA gyrase inhibitor YacG -
  CFter6_RS22005 (CFter6_4806) mutT 5026920..5027333 (-) 414 WP_061541725.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62834.63 Da        Isoelectric Point: 5.1140

>NTDB_id=160868 CFter6_RS21975 WP_014007897.1 5021305..5023029(+) (pilB) [Collimonas fungivorans strain Ter6]
MAAVFPNANSTSPISGLARALLQAGRLSVPQLEALHKKASESQTPFISALLESGQLDARSLALFCAETFGYPQLDLSVLN
LGMLPEKAIDAKLMETHSVLALAKRGNKIYVAFSDPTNIQALDQIKFQTELTVEPIIVEHPVLLKQIQKLVKSAEQSLNE
MVGDEQEINFVEEDAVAVADAAATDIDDAPVVRFLQKILTDAINQGASDLHFEPFEKFYRIRFRVDGVLRDMAQPPLSIK
EKLVSRIKVISKLDISEKRVPQDGRMRLAVSKTRAIDFRVSTLPTLFGEKVVMRILDPSQAQMGIDALGYDPDQKEILME
AIQRPYGMVLVTGPTGSGKTVSLYTCLNILNQPGINISTAEDPAEINLPGVNQVNINDRAGLTFPVALKAFLRQDPDIIM
VGEIRDLETADISIKAAQTGHMVFSTLHTNDAPSTLTRLMNMGVPAFNIASSVILITAQRLARRLCSCKQPITVQEEALL
EAGFTEDDLDGSWMPYKAVGCDRCNGTGYKGRVGIYQIMPITEEIERIILAHGTALEIEAQAKREGVKTLRLSGLMKVKQ
GLTSLEEVLGCTNI

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=160868 CFter6_RS21975 WP_014007897.1 5021305..5023029(+) (pilB) [Collimonas fungivorans strain Ter6]
ATGGCAGCAGTATTTCCCAACGCTAATTCCACCAGCCCGATCTCCGGACTTGCCCGCGCATTGCTGCAAGCCGGACGTCT
TTCCGTACCCCAGCTGGAGGCCTTGCACAAAAAGGCTAGCGAGAGTCAAACGCCGTTTATCTCCGCCTTGCTGGAAAGCG
GGCAGCTGGATGCCCGTTCGCTTGCCCTGTTTTGCGCCGAGACCTTCGGTTATCCGCAGCTGGACCTGAGCGTACTCAAC
CTCGGCATGCTGCCGGAAAAAGCCATCGACGCCAAGCTGATGGAAACCCATTCCGTATTGGCCCTGGCGAAACGCGGCAA
CAAGATCTATGTCGCCTTTTCCGATCCGACCAATATCCAGGCGCTGGACCAGATCAAGTTCCAGACCGAGCTGACGGTTG
AACCGATCATCGTCGAACACCCGGTGCTGCTGAAACAGATCCAGAAACTGGTCAAGTCGGCCGAGCAAAGCCTCAACGAA
ATGGTCGGCGACGAGCAGGAAATCAACTTCGTCGAAGAGGATGCGGTAGCGGTTGCCGATGCTGCGGCTACCGATATCGA
CGATGCGCCGGTAGTCAGGTTCTTGCAGAAGATACTGACCGACGCCATCAACCAGGGCGCGTCCGACTTGCATTTCGAGC
CGTTCGAAAAATTCTACCGTATCCGTTTCCGGGTCGACGGCGTCTTGCGCGACATGGCGCAGCCGCCGCTGTCGATCAAG
GAAAAACTGGTATCCCGCATCAAGGTGATCTCCAAGCTGGATATTTCCGAAAAGCGGGTGCCGCAAGATGGCCGCATGCG
CCTGGCGGTATCCAAGACCCGCGCAATCGATTTCCGGGTCAGCACATTGCCGACCTTGTTTGGCGAAAAAGTCGTAATGC
GGATCCTGGACCCGAGCCAGGCCCAGATGGGGATTGACGCGCTGGGTTACGACCCGGACCAGAAAGAAATCCTGATGGAG
GCTATCCAGCGTCCCTACGGCATGGTGCTGGTGACCGGGCCGACCGGTTCCGGCAAAACCGTATCGCTGTATACCTGCCT
GAACATCCTGAACCAGCCCGGCATCAACATTTCCACCGCCGAAGATCCAGCCGAAATCAATTTGCCCGGCGTGAACCAGG
TCAACATCAACGACCGTGCCGGACTGACTTTCCCGGTGGCGCTGAAAGCCTTCCTGCGGCAGGATCCCGACATCATCATG
GTCGGTGAAATCCGCGACCTGGAAACCGCCGACATCTCGATCAAGGCCGCGCAAACCGGGCACATGGTGTTTTCCACACT
GCACACCAACGATGCGCCATCGACGCTGACGCGTCTGATGAACATGGGGGTGCCGGCCTTCAATATCGCCTCGTCGGTGA
TCCTGATCACTGCACAGCGGCTGGCGCGCCGCCTGTGCAGCTGCAAGCAGCCGATTACCGTCCAGGAAGAAGCCTTGCTG
GAAGCCGGCTTCACCGAGGACGACCTCGACGGCAGCTGGATGCCGTACAAGGCGGTCGGCTGCGATCGCTGCAACGGCAC
AGGATACAAAGGGCGGGTCGGCATCTACCAGATCATGCCGATCACCGAAGAAATAGAACGGATTATCCTGGCGCACGGCA
CGGCGCTGGAAATCGAAGCGCAGGCTAAGCGCGAGGGGGTCAAGACCCTGCGTTTGTCAGGATTGATGAAGGTTAAACAA
GGGTTGACCAGCCTCGAAGAAGTGCTTGGCTGCACCAATATCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-153)

Type II/IV secretion system protein (T2SSE)

(197-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.816

99.477

0.545
  pilB Acinetobacter baylyi ADP1

53.739

100

0.538
  pilF Neisseria gonorrhoeae MS11

53.792

98.78

0.531
  pilB Legionella pneumophila strain ERS1305867

52.407

94.077

0.493
  pilB Vibrio cholerae strain A1552

47.163

98.258

0.463
  pilB Vibrio parahaemolyticus RIMD 2210633

46.903

98.432

0.462
  pilB Vibrio campbellii strain DS40M4

44.876

98.606

0.443
  pilF Thermus thermophilus HB27

40.449

93.031

0.376
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.414

92.683

0.375

Multiple sequence alignment