Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PP2015_RS02915 Genome accession   NZ_CP013187
Coordinates   654944..656626 (-) Length   560 a.a.
NCBI ID   WP_058028854.1    Uniprot ID   -
Organism   Pseudoalteromonas phenolica strain KCTC 12086     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 649944..661626
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PP2015_RS02885 - 650027..650926 (+) 900 WP_083496495.1 cation diffusion facilitator family transporter -
  PP2015_RS02890 (PP2015_565) - 650878..651843 (-) 966 WP_058028850.1 alpha/beta fold hydrolase -
  PP2015_RS02895 (PP2015_566) - 651840..653237 (-) 1398 WP_083496496.1 ATP-binding protein -
  PP2015_RS02900 (PP2015_567) - 653218..653901 (-) 684 WP_058028851.1 response regulator transcription factor -
  PP2015_RS02905 (PP2015_568) - 654062..654502 (+) 441 WP_058028852.1 Spy/CpxP family protein refolding chaperone -
  PP2015_RS02910 (PP2015_569) yacG 654603..654842 (-) 240 WP_058028853.1 DNA gyrase inhibitor YacG -
  PP2015_RS02915 (PP2015_570) pilB 654944..656626 (-) 1683 WP_058028854.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PP2015_RS02920 (PP2015_571) coaE 656764..657372 (-) 609 WP_058028855.1 dephospho-CoA kinase -
  PP2015_RS02925 (PP2015_572) pilD 657404..658294 (-) 891 WP_058028856.1 prepilin peptidase Machinery gene
  PP2015_RS02930 (PP2015_573) pilC 658330..659565 (-) 1236 WP_058028857.1 type II secretion system F family protein Machinery gene
  PP2015_RS02935 (PP2015_574) - 659617..660117 (-) 501 WP_058028858.1 pilin -
  PP2015_RS02940 (PP2015_575) nadC 660680..661546 (-) 867 WP_058028859.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 560 a.a.        Molecular weight: 62327.86 Da        Isoelectric Point: 5.7347

>NTDB_id=160502 PP2015_RS02915 WP_058028854.1 654944..656626(-) (pilB) [Pseudoalteromonas phenolica strain KCTC 12086]
MEHHSPLLRKFITLGRVTAEQLKLKQAEAQSTAELICLCSGMTSQELAEQCLDLFRVPMFDLSDFDVEQLPKELIKEKLI
RQHHLLPLVKKGRKLYIATSDPTDYGAFENFEFSTGLQCEVVVVDYKLLEQKIDQLFDATGGLSMSEDDFKEFSGMDVEE
TQQQQDTNDEKDKDDAPIIVYINKILMDAIKKGASDLHFEPYEHKYRIRFRIDGMLHEMASPPTSLATRLSARIKVMSRL
DIAEKRKPQDGRIKLKISDRKSIDFRVSTLPTLWGEKIVMRILDSSSAMLGIDVLGYEPEQKALYMDALAQPQGMILVTG
PTGSGKTVSLYTGLNILNKPERNISTAEDPVEINLEGINQVQINPKAEMTFANALKAFLRQDPDVVMVGEIRDLETAEIS
IKAAQTGHLVMSTLHTNSAPETLTRLLNMGVPAYNVASSISLIIAQRLARRLCPKCKTPETLPEEELNRQGFIKAGISEV
TLYKANGCEHCTEGYKGRVGVYEVVKITPEIAQVIMEGGNSIQIAEMAVKAGFANLRISGLKKAAAGVTSLAEINRVTSY

Nucleotide


Download         Length: 1683 bp        

>NTDB_id=160502 PP2015_RS02915 WP_058028854.1 654944..656626(-) (pilB) [Pseudoalteromonas phenolica strain KCTC 12086]
ATGGAACATCACTCTCCCCTTTTACGGAAGTTTATTACGCTCGGCCGTGTGACCGCTGAGCAATTAAAATTAAAACAAGC
CGAGGCACAGTCAACGGCAGAGCTGATCTGTCTATGCAGTGGGATGACCAGTCAAGAGTTAGCTGAACAATGCTTAGATC
TGTTCAGAGTACCGATGTTCGACTTAAGCGACTTTGACGTTGAACAGCTGCCAAAAGAGCTCATAAAAGAAAAACTGATA
CGTCAGCATCATTTACTACCTCTAGTAAAAAAAGGTCGTAAACTCTATATTGCCACCTCTGATCCTACTGATTACGGTGC
ATTTGAAAACTTTGAGTTTAGTACTGGTCTTCAATGCGAAGTCGTAGTTGTTGATTACAAACTGCTCGAACAAAAAATAG
ATCAATTATTTGATGCCACTGGTGGCCTGTCCATGAGCGAGGATGACTTTAAAGAGTTCTCGGGCATGGATGTTGAAGAG
ACTCAACAGCAACAAGACACCAACGATGAAAAAGACAAAGATGATGCCCCTATTATTGTCTACATCAATAAAATCTTGAT
GGATGCGATTAAAAAAGGGGCGTCAGATTTACACTTTGAACCTTACGAGCATAAATACCGCATTCGTTTTCGTATTGATG
GCATGCTGCATGAAATGGCCAGCCCGCCAACTAGCTTAGCAACTCGGTTATCTGCTCGTATCAAGGTTATGTCTCGGTTA
GACATCGCCGAAAAACGTAAACCGCAAGACGGCCGTATCAAATTAAAGATCTCTGATCGTAAAAGCATTGATTTTCGTGT
CAGTACCCTGCCCACTTTATGGGGAGAAAAAATCGTAATGCGTATTCTTGACTCATCAAGTGCTATGTTAGGCATTGATG
TGTTAGGTTACGAGCCTGAGCAAAAAGCACTTTACATGGATGCTTTAGCACAGCCGCAAGGTATGATCTTAGTAACCGGC
CCAACAGGTTCGGGTAAAACAGTTTCACTCTATACAGGTTTAAATATTCTTAATAAACCAGAGCGCAATATCAGTACTGC
GGAAGATCCAGTTGAAATTAACCTCGAAGGTATCAACCAAGTTCAGATCAACCCCAAAGCAGAGATGACTTTTGCCAACG
CGCTCAAAGCCTTCTTACGTCAAGATCCAGATGTCGTGATGGTTGGTGAGATCCGTGACTTAGAAACCGCAGAAATCTCT
ATTAAGGCTGCACAAACAGGTCACTTGGTGATGAGTACACTACACACCAATTCGGCACCAGAAACGTTAACTCGTCTACT
GAATATGGGTGTACCAGCATATAACGTAGCAAGCTCGATCAGCTTGATCATCGCACAGCGTCTAGCACGTCGTTTATGTC
CTAAGTGTAAAACGCCTGAAACTTTGCCTGAAGAAGAGCTTAATCGCCAAGGATTTATAAAAGCAGGGATCAGCGAAGTC
ACGCTGTATAAAGCCAATGGCTGTGAACACTGTACCGAGGGCTATAAAGGCCGTGTCGGTGTGTATGAAGTAGTGAAAAT
CACACCTGAAATTGCACAAGTTATTATGGAAGGTGGCAACTCGATTCAAATCGCTGAAATGGCGGTAAAAGCAGGATTCG
CCAATTTACGTATTTCGGGTCTGAAAAAAGCCGCTGCAGGCGTCACCTCATTAGCTGAAATAAACCGAGTAACCAGTTAC
TAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(184-451)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.81

100

0.566
  pilB Acinetobacter baylyi ADP1

55.971

100

0.561
  pilB Vibrio cholerae strain A1552

53.417

99.286

0.53
  pilB Legionella pneumophila strain ERS1305867

53.676

97.143

0.521
  pilB Vibrio parahaemolyticus RIMD 2210633

53.296

94.821

0.505
  pilB Vibrio campbellii strain DS40M4

52.372

94.107

0.493
  pilF Neisseria gonorrhoeae MS11

48.036

100

0.48
  pilB/pilB1 Synechocystis sp. PCC 6803

37.56

100

0.418

Multiple sequence alignment