Detailed information NTG0160503

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: PP2015_RS02925
• Genome accession: NZ_CP013187
• Coordinates: 657404..658294 (-)
• Length: 296 a.a.
• NCBI ID: WP_058028856.1
• Uniprot ID: A0A0S2JZ15
• Organism: Pseudoalteromonas phenolica strain KCTC 12086
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 652404..663294

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PP2015_RS02900 (PP2015_567)	-	653218..653901 (-)	684	WP_058028851.1	response regulator transcription factor	-
PP2015_RS02905 (PP2015_568)	-	654062..654502 (+)	441	WP_058028852.1	Spy/CpxP family protein refolding chaperone	-
PP2015_RS02910 (PP2015_569)	yacG	654603..654842 (-)	240	WP_058028853.1	DNA gyrase inhibitor YacG	-
PP2015_RS02915 (PP2015_570)	pilB	654944..656626 (-)	1683	WP_058028854.1	type IV-A pilus assembly ATPase PilB	Machinery gene
PP2015_RS02920 (PP2015_571)	coaE	656764..657372 (-)	609	WP_058028855.1	dephospho-CoA kinase	-
PP2015_RS02925 (PP2015_572)	pilD	657404..658294 (-)	891	WP_058028856.1	prepilin peptidase	Machinery gene
PP2015_RS02930 (PP2015_573)	pilC	658330..659565 (-)	1236	WP_058028857.1	type II secretion system F family protein	Machinery gene
PP2015_RS02935 (PP2015_574)	-	659617..660117 (-)	501	WP_058028858.1	pilin	-
PP2015_RS02940 (PP2015_575)	nadC	660680..661546 (-)	867	WP_058028859.1	carboxylating nicotinate-nucleotide diphosphorylase	-
PP2015_RS02945 (PP2015_576)	ampD	661688..662245 (+)	558	WP_405127322.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
PP2015_RS02950 (PP2015_577)	ampE	662253..663104 (+)	852	WP_058028860.1	beta-lactamase regulator AmpE	-

Sequence

Protein

• Length: 296 a.a.
• Molecular weight: 32904.16 Da
• Isoelectric Point: 7.2746

>NTDB_id=160503 PP2015_RS02925 WP_058028856.1 657404..658294(-) (pilD) [Pseudoalteromonas phenolica strain KCTC 12086]
MQEVIYLMQSQQWFFLLTVGLVSLCIGSFLNVVIYRLPIMMQREWQQECRLSLADELANSSKQKTSSNDTFNLIKPNSTC
PHCKTPIKAWHNIPLLGWLMLKGKCASCSAKISIRYPLVELGTALASLWIALHFGATELSFLYIFITWVLMALIFIDIDH
MLLPDQLTLPLVWLALIASVMGYTIAPSEAIIGAAVGYLSLWTVFWAFKLLTGKEGMGYGDFKLLAIFGALLGWQAILTI
VLLSSVVGAIIGSIQLAVQGKEHSTPIPFGPYLAIAGWLAMLYGTEIQTWYFSLLS

Nucleotide

• Length: 891 bp

>NTDB_id=160503 PP2015_RS02925 WP_058028856.1 657404..658294(-) (pilD) [Pseudoalteromonas phenolica strain KCTC 12086]
ATGCAAGAAGTAATTTATTTAATGCAAAGCCAACAGTGGTTTTTTCTTTTGACCGTTGGTTTAGTTAGCCTGTGTATTGG
TAGCTTTTTAAATGTGGTGATTTACCGACTGCCAATCATGATGCAACGTGAGTGGCAGCAAGAGTGCCGTTTATCGTTGG
CCGATGAATTGGCAAACTCTAGCAAGCAAAAAACATCGAGCAACGACACTTTCAACCTAATTAAACCTAACTCGACTTGC
CCTCATTGCAAAACCCCAATTAAAGCTTGGCATAATATTCCCTTGTTGGGCTGGCTGATGTTAAAAGGCAAATGTGCTAG
TTGCTCAGCAAAAATCTCAATTCGTTACCCGCTAGTAGAGTTAGGAACTGCCCTTGCCTCACTTTGGATTGCATTACACT
TTGGCGCAACTGAGCTCAGCTTTCTTTATATTTTTATCACTTGGGTACTCATGGCGCTTATTTTTATCGATATCGATCAC
ATGTTATTACCCGACCAGCTCACGCTTCCTTTGGTTTGGCTGGCATTAATTGCCAGTGTCATGGGCTATACCATTGCCCC
CAGCGAAGCCATTATCGGTGCTGCAGTAGGCTACTTAAGTTTATGGACGGTATTTTGGGCATTTAAGCTACTCACAGGCA
AAGAAGGCATGGGATATGGTGATTTTAAACTGTTAGCTATTTTTGGTGCACTATTGGGTTGGCAGGCCATTTTGACCATA
GTTTTACTCTCAAGTGTTGTGGGCGCCATCATTGGTAGCATTCAATTAGCTGTACAAGGTAAAGAGCACAGTACACCTAT
TCCATTTGGGCCATATTTGGCCATCGCTGGTTGGCTAGCGATGCTCTATGGCACCGAGATTCAAACTTGGTACTTTTCTC
TTTTAAGCTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (145-252)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (22-134)

3D structure

• AlphaFold DB: A0A0S2JZ15

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	54.61	95.27	0.52
pilD	Acinetobacter nosocomialis M2	49.32	99.324	0.49
pilD	Vibrio campbellii strain DS40M4	50.347	97.297	0.49
pilD	Acinetobacter baumannii D1279779	48.98	99.324	0.486
pilD	Neisseria gonorrhoeae MS11	43.346	88.851	0.385