Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CMT41_RS15495 Genome accession   NZ_CP013145
Coordinates   3655329..3657029 (+) Length   566 a.a.
NCBI ID   WP_057182476.1    Uniprot ID   A0A0S2JJD1
Organism   Colwellia sp. MT41     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3650329..3662029
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CMT41_RS15485 (CMT41_15485) - 3651245..3652717 (-) 1473 WP_058027772.1 oligosaccharide flippase family protein -
  CMT41_RS15490 (CMT41_15490) - 3653043..3655058 (-) 2016 WP_058027773.1 acyltransferase family protein -
  CMT41_RS15495 (CMT41_15495) pilB 3655329..3657029 (+) 1701 WP_057182476.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CMT41_RS15500 (CMT41_15500) pilC 3657145..3658401 (+) 1257 WP_057182477.1 type II secretion system F family protein Machinery gene
  CMT41_RS15505 (CMT41_15505) pilD 3658565..3659425 (+) 861 WP_057182704.1 A24 family peptidase Machinery gene
  CMT41_RS15510 (CMT41_15510) coaE 3659444..3660052 (+) 609 WP_058027774.1 dephospho-CoA kinase -
  CMT41_RS15515 (CMT41_15515) zapD 3660117..3660869 (+) 753 WP_058027775.1 cell division protein ZapD -
  CMT41_RS15520 (CMT41_15520) yacG 3660982..3661218 (+) 237 WP_058027776.1 DNA gyrase inhibitor YacG -
  CMT41_RS15525 (CMT41_15525) mutT 3661229..3661657 (-) 429 WP_058027777.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62319.79 Da        Isoelectric Point: 5.0197

>NTDB_id=160312 CMT41_RS15495 WP_057182476.1 3655329..3657029(+) (pilB) [Colwellia sp. MT41]
MKGSHQQASVLSALSKQNLIPLESIEDITADFVGKSKPFIRFLIENKNLDGKSIAKVLSKSFGYPQIQLSNFDTTLVPDG
VRNEKLITKHNALPLYLRGKVLFVAMSDPTNLDALEEIQFNTGYNTELVLCEDTSLQACIEKVLEDESDALDISDIDSEE
LAGIDVAEAKKEEDKTNSEDDAPIVVFINKILLDAIKKGASDLHFEPYEKTFRIRFRIDGILSEIAKPPVALASRMAARL
KVMSKLDIAERRVPQDGRIKLALSKKKSIDFRVSTLPTMWGEKIVMRILDSSSAMLGIDMLGYDPEQKKIYMDALEQPQG
MILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGINQVQINNRAGLTFPIALRSFLRQDPDVIMVGEIRDL
ETAEISIKAAQTGHLVLSTLHTNSAAETLTRLLNMGVPAYNVASSVSIIIAQRLARRLCPQCKEEEPLSEMLLAEEGFPA
DKLSEIKLFKAVGCSHCTGGYKGRVGIYEVIKISPIIATIIMEGGNSLDIAKQCQKEGYNNLRQSGLIKAMNGMTSLEEI
NRVTSS

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=160312 CMT41_RS15495 WP_057182476.1 3655329..3657029(+) (pilB) [Colwellia sp. MT41]
ATGAAAGGAAGTCATCAACAAGCCAGTGTTTTATCGGCACTATCCAAGCAGAACCTTATTCCTCTAGAGTCAATTGAAGA
CATTACCGCTGATTTTGTCGGTAAAAGTAAGCCATTTATTCGTTTTTTAATAGAAAATAAAAACCTTGATGGTAAAAGTA
TCGCCAAAGTACTGTCTAAAAGCTTTGGCTATCCACAAATTCAACTGTCAAATTTTGATACCACTTTAGTACCTGACGGT
GTGCGCAATGAAAAATTAATTACCAAGCACAACGCCCTACCACTATATCTGCGGGGTAAAGTGCTCTTTGTTGCTATGTC
AGATCCCACGAATCTAGATGCGTTAGAAGAAATTCAGTTTAATACTGGCTACAACACTGAGTTAGTTTTATGTGAAGATA
CTAGCTTACAAGCCTGTATTGAAAAAGTATTAGAAGATGAAAGTGATGCCCTAGATATCAGTGATATTGATTCCGAAGAG
TTAGCGGGTATAGATGTAGCAGAAGCCAAGAAAGAAGAAGACAAAACAAATAGTGAAGATGACGCACCTATTGTGGTTTT
TATTAACAAGATTTTACTCGATGCCATTAAAAAAGGCGCTTCTGATTTACATTTTGAACCCTATGAAAAAACATTCCGCA
TTCGCTTTCGTATTGATGGCATATTATCTGAGATAGCTAAACCTCCTGTAGCTCTAGCGTCAAGAATGGCTGCGCGCCTA
AAAGTCATGTCTAAATTAGATATCGCTGAGCGACGGGTGCCGCAAGATGGCCGGATAAAATTAGCATTATCAAAGAAAAA
ATCTATCGATTTTCGTGTCAGCACCTTACCCACTATGTGGGGTGAAAAAATTGTAATGCGTATCCTAGACTCATCAAGTG
CCATGCTGGGTATCGATATGCTTGGTTATGATCCTGAACAGAAAAAAATATACATGGACGCATTAGAGCAGCCACAAGGC
ATGATATTAGTTACCGGGCCAACAGGCTCAGGTAAGACGGTATCCTTGTATACCGGGTTAAATATTCTCAATACTGAAGA
ACGTAATATCTCTACTGCTGAAGATCCGGTCGAAATTAACCTTGAAGGTATTAACCAGGTACAAATTAATAATCGTGCCG
GTCTAACCTTCCCCATCGCCCTGCGCTCTTTTTTACGACAAGATCCAGATGTTATTATGGTGGGTGAAATACGAGACCTT
GAAACCGCTGAGATTTCCATCAAAGCCGCACAAACTGGTCATTTAGTGTTATCGACTCTTCATACTAACTCAGCGGCAGA
AACGCTAACCCGTTTATTAAATATGGGTGTGCCTGCTTATAATGTTGCCAGCTCTGTTTCAATAATAATAGCCCAGCGAT
TAGCACGGAGATTATGCCCTCAGTGTAAGGAAGAGGAGCCTCTTTCGGAGATGCTATTAGCCGAAGAAGGATTTCCTGCT
GATAAACTTAGCGAGATAAAATTATTTAAAGCGGTAGGTTGCAGCCACTGTACTGGCGGTTATAAAGGCCGCGTAGGAAT
TTATGAAGTGATAAAGATTAGTCCAATCATAGCGACTATTATTATGGAGGGCGGCAACTCTCTCGATATCGCTAAGCAAT
GTCAAAAAGAAGGCTACAACAACCTACGTCAATCAGGGTTAATTAAAGCCATGAATGGTATGACCAGCTTAGAAGAAATT
AACCGCGTGACTAGCTCTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-148)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0S2JJD1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.519

95.406

0.558
  pilB Acinetobacter baumannii D1279779

58.712

93.286

0.548
  pilB Legionella pneumophila strain ERS1305867

56.874

93.816

0.534
  pilB Vibrio cholerae strain A1552

52.847

99.293

0.525
  pilB Vibrio campbellii strain DS40M4

52.741

93.463

0.493
  pilB Vibrio parahaemolyticus RIMD 2210633

53.578

91.343

0.489
  pilF Neisseria gonorrhoeae MS11

50.279

94.876

0.477
  pilB/pilB1 Synechocystis sp. PCC 6803

42.231

88.693

0.375
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.033

95.23

0.362

Multiple sequence alignment