Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   AO356_RS09410 Genome accession   NZ_CP012831
Coordinates   2127317..2129404 (-) Length   695 a.a.
NCBI ID   WP_060739557.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain FW300-N2C3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2122317..2134404
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AO356_RS09395 (AO356_09380) - 2123929..2125503 (-) 1575 WP_060739554.1 AAA family ATPase -
  AO356_RS09400 (AO356_09385) aroB 2125514..2126614 (-) 1101 WP_060739555.1 3-dehydroquinate synthase -
  AO356_RS09405 (AO356_09390) aroK 2126794..2127312 (-) 519 WP_060739556.1 shikimate kinase AroK -
  AO356_RS09410 (AO356_09395) pilQ 2127317..2129404 (-) 2088 WP_060739557.1 type IV pilus secretin PilQ Machinery gene
  AO356_RS09415 (AO356_09400) - 2129401..2129949 (-) 549 WP_060739558.1 pilus assembly protein PilP -
  AO356_RS09420 (AO356_09405) pilO 2129946..2130569 (-) 624 WP_060739559.1 type 4a pilus biogenesis protein PilO -
  AO356_RS09425 (AO356_09410) - 2130566..2131132 (-) 567 WP_060739560.1 PilN domain-containing protein -
  AO356_RS09430 (AO356_09415) comM 2131132..2132196 (-) 1065 WP_060739561.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 695 a.a.        Molecular weight: 75075.30 Da        Isoelectric Point: 6.1178

>NTDB_id=157808 AO356_RS09410 WP_060739557.1 2127317..2129404(-) (pilQ) [Pseudomonas fluorescens strain FW300-N2C3]
MNRFLSAFGISLWIALLSPMVHAASLKTLDVAALPGDRVELKLTFDGPPPPPRGYTTEQPARIALDLPGVTNQLASKHRD
LGGGNARSATVVEAGDRTRLIIGLTQLAPYDTRVEGNSLFVVVGKGAAAKNTAGKSVAQAPRPAVANAVPARAGRPVGKA
IRGVDFQRGTQGEGNVVIDLSDPSIAPDIQEREGKIILNFARTQLPEPLRVRLDVKDFATPVQFVNASAGSDRATISIEP
SGTFDYSTYQTDNKLTVSVRPMTVDDLQKRNADRAAYSGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRL
QNVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLAPLRRELLQVNYAKAADIAKLFQSVTSA
EAKADERGTITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNSSG
VTTGTSTTIGTPGSTGTNQPFVDLGASGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTE
IPYQEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGV
FSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2088 bp        

>NTDB_id=157808 AO356_RS09410 WP_060739557.1 2127317..2129404(-) (pilQ) [Pseudomonas fluorescens strain FW300-N2C3]
ATGAACAGGTTTTTGTCAGCCTTCGGTATTTCGCTATGGATAGCGCTGTTGTCGCCGATGGTTCACGCGGCCAGTCTGAA
GACGCTGGATGTCGCGGCGTTGCCGGGCGACCGCGTCGAGTTGAAGCTCACCTTCGACGGGCCGCCGCCGCCCCCTCGGG
GCTACACCACCGAACAACCGGCCCGGATTGCCCTGGACCTGCCAGGCGTGACCAATCAACTGGCCAGCAAGCACCGCGAC
CTGGGCGGGGGCAATGCGCGCAGCGCCACCGTGGTCGAGGCAGGCGATCGCACCCGGTTGATCATCGGTCTGACCCAGCT
GGCGCCGTACGACACTCGGGTCGAAGGCAATAGCCTGTTCGTGGTGGTTGGCAAGGGCGCCGCGGCGAAGAACACTGCTG
GGAAGAGCGTCGCCCAGGCCCCGCGCCCGGCGGTGGCCAACGCGGTGCCCGCGAGGGCCGGTAGGCCGGTGGGCAAGGCG
ATTCGTGGGGTGGATTTCCAGCGGGGAACCCAGGGCGAAGGCAATGTGGTGATCGATTTGTCCGATCCGTCCATCGCCCC
GGACATCCAGGAACGCGAAGGCAAGATCATCCTCAATTTCGCCAGGACCCAATTGCCCGAACCATTGCGGGTGCGCCTGG
ACGTCAAGGACTTCGCCACCCCGGTGCAATTCGTCAATGCCAGCGCCGGCAGCGACCGGGCGACCATCAGCATCGAGCCC
AGTGGCACCTTCGACTACTCGACCTACCAGACCGACAACAAACTCACCGTCAGCGTCCGGCCCATGACTGTCGACGACCT
GCAAAAACGCAATGCTGACCGCGCGGCCTACAGCGGCGAGAAGCTCTCGCTCAACTTCCAGGACATCGAAGTGCGTTCGG
TCCTGCAACTGATTGCCGATTTCACCAACCTGAACCTGGTGGCCAGCGACACGGTGCAGGGCGGCATTACCCTGCGCTTG
CAGAATGTGCCTTGGGACCAGGCGCTGGACCTGGTGCTCAAGACCAAGGGTTTGGACAAGCGCAAGGTCGGTAACGTGCT
GCTGGTGGCCCCGGCCGATGAAATCGCCGCGCGTGAACGCCAGGAACTGGAGTCGCAGAAGCAGATCGCCGACCTGGCAC
CGCTGCGGCGTGAGCTGCTGCAGGTCAACTATGCGAAGGCGGCGGACATCGCCAAGCTGTTCCAGTCAGTGACCAGTGCC
GAGGCCAAAGCCGACGAGCGCGGCACGATCACGGTGGATGAGCGAACCAACAACATCATTGCCTACCAGACCCAGGACCG
GCTCGACGAACTGCGCCGGATCGTGGCGCAGCTGGATATTCCGGTGCGCCAGGTGATGATCGAGGCACGGATTGTCGAAG
CCAACGTCGACTACGACAAAAGCCTGGGCGTGCGCTGGGGAGGGTCGATCCAGAACAAGGGCAACTGGAACTCCTCGGGG
GTGACCACTGGCACCTCCACCACCATCGGCACGCCGGGCAGTACCGGCACCAACCAGCCGTTCGTCGACCTGGGTGCCTC
GGGCAATACGTCGGGGATCGGCATTGCGTTCATCACCGACAACGTCTTGCTGGACCTTGAGCTGACGGCCATGGAAAAAA
CCGGCAACGGCGAAATCGTCTCCCAGCCCAAGGTGGTGACGTCCGACAAGGAGACGGCAAAGATCCTCAAGGGCACGGAA
ATCCCCTACCAGGAAGCCAGTTCCAGCGGCGCCACCTCGGTGTCGTTCAAGGAGGCCTCGCTGTCGCTGGAGGTGACCCC
GCAGATCACCCCGGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGACTACCTGAACAAGGTGCAGG
ACGTGCCGCCGATCAAGAAAAACGAAGTCAACGCCAAGGTCCTGGTCAATGATGGCGAGACCATTGTGATTGGCGGAGTT
TTCTCAAATACTCAGAGCAAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCG
TGATGTGGTTTCGGAGAAAAAATCCGAGCTGCTGGTGTTCCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGA
GTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(305-351)

Bacterial type II and III secretion system protein (Secretin)

(530-686)

Bacterial type II/III secretion system short domain (Secretin_N)

(379-444)

AMIN domain (AMIN)

(38-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.007

100

0.751
  comQ Acinetobacter baylyi ADP1

41.954

100

0.42
  pilQ Acinetobacter baumannii D1279779

38.904

100

0.419
  pilQ Acinetobacter baumannii strain A118

38.904

100

0.419
  pilQ Legionella pneumophila strain Lp02

38.733

97.698

0.378
  pilQ Legionella pneumophila strain ERS1305867

38.586

97.698

0.377

Multiple sequence alignment