Detailed information NTG0157809

Bioinformatically predicted

Overview

• Name: comM
• Type: Machinery gene
• Locus tag: AO356_RS09430
• Genome accession: NZ_CP012831
• Coordinates: 2131132..2132196 (-)
• Length: 354 a.a.
• NCBI ID: WP_060739561.1
• Uniprot ID: A0A2K4FSG9
• Organism: Pseudomonas fluorescens strain FW300-N2C3
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2126132..2137196

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AO356_RS09405 (AO356_09390)	aroK	2126794..2127312 (-)	519	WP_060739556.1	shikimate kinase AroK	-
AO356_RS09410 (AO356_09395)	pilQ	2127317..2129404 (-)	2088	WP_060739557.1	type IV pilus secretin PilQ	Machinery gene
AO356_RS09415 (AO356_09400)	-	2129401..2129949 (-)	549	WP_060739558.1	pilus assembly protein PilP	-
AO356_RS09420 (AO356_09405)	pilO	2129946..2130569 (-)	624	WP_060739559.1	type 4a pilus biogenesis protein PilO	-
AO356_RS09425 (AO356_09410)	-	2130566..2131132 (-)	567	WP_060739560.1	PilN domain-containing protein	-
AO356_RS09430 (AO356_09415)	comM	2131132..2132196 (-)	1065	WP_060739561.1	pilus assembly protein PilM	Machinery gene
AO356_RS09435 (AO356_09420)	-	2132405..2134849 (+)	2445	WP_203225789.1	penicillin-binding protein 1A	-
AO356_RS09440 (AO356_09425)	-	2135097..2136365 (-)	1269	WP_053117649.1	malic enzyme-like NAD(P)-binding protein	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38121.77 Da
• Isoelectric Point: 4.9112

>NTDB_id=157809 AO356_RS09430 WP_060739561.1 2131132..2132196(-) (comM) [Pseudomonas fluorescens strain FW300-N2C3]
MLRLFNKKAHALLGIDISSTSVKLLELSRQGERYRVESYAVEPLPANAVIEKNIAELEGVGQALSRLLAKAKTPLRSVAV
AVAGSAVITKIIEMDAGLSDDDMENQLKIEADQYIPYPLDEVAIDFEVVGVSPRSAERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERAFGLLATQLAASQERLTVAVIDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLTP
EQAGLAKKQGGLPDDYLSEVLQPFREALVQQVSRSLQFFFASGQYSAVDHILLAGGTASVAGLDRLIEQRLGTPTQVANP
FTNMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=157809 AO356_RS09430 WP_060739561.1 2131132..2132196(-) (comM) [Pseudomonas fluorescens strain FW300-N2C3]
GTGCTACGACTCTTCAATAAAAAAGCCCATGCGCTTCTGGGGATCGACATCAGTTCGACGTCGGTGAAGCTGCTCGAGTT
GAGTCGCCAGGGCGAGCGATACCGCGTCGAGTCCTACGCGGTCGAACCGTTGCCGGCCAACGCCGTGATCGAGAAGAACA
TCGCCGAGCTCGAAGGGGTGGGCCAGGCGTTGTCGCGGCTGTTGGCCAAGGCCAAGACGCCCTTGCGCAGCGTGGCGGTG
GCCGTGGCGGGTTCGGCGGTGATCACCAAGATCATCGAGATGGATGCGGGCCTGTCCGACGACGACATGGAGAACCAGCT
CAAGATCGAAGCCGATCAGTACATTCCCTATCCGCTGGATGAGGTGGCCATTGATTTCGAGGTGGTGGGCGTTTCGCCAC
GCAGCGCCGAGCGCGTCGAAGTGCTGCTGGCGGCCTGTCGCAAGGAAAACGTCGAGGTTCGCGAGGCCGCGTTGGCGCTC
GCCGGGCTGACGGCCCGGGTGGTCGATGTGGAGGCTTATGCGCTGGAGCGAGCGTTTGGCCTGCTCGCCACGCAATTGGC
CGCGTCCCAGGAGCGGCTGACGGTGGCGGTCATCGACATCGGCGCCACCATGACCACCCTCAGCGTGTTGCACAACGGGC
GCATCATCTATACCCGCGAACAATTGTTCGGCGGTCGCCAGTTGACCGAGGAGATCCAGCGCCGCTACGGCCTGACACCC
GAGCAGGCCGGCCTGGCAAAGAAGCAGGGTGGGTTGCCGGACGATTATCTCAGCGAAGTGCTGCAACCTTTTCGCGAGGC
CCTGGTGCAGCAGGTCTCGCGCTCCCTGCAATTCTTCTTCGCCTCGGGCCAGTACAGCGCGGTGGACCACATTCTGCTGG
CCGGGGGCACGGCTTCCGTCGCTGGCCTGGATCGGCTGATCGAGCAGCGCCTGGGCACGCCGACCCAGGTGGCCAACCCG
TTTACCAACATGGCCCTGAGCAGCAAGGTCAATGCCGGTGCCCTGGCCAGTGATGCCCCGGCACTGATGATTGCCTGTGG
GCTGGCCCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A2K4FSG9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comM	Acinetobacter nosocomialis M2	55.367	100	0.554
pilM	Acinetobacter baumannii D1279779	55.367	100	0.554
comM	Acinetobacter baylyi ADP1	54.52	100	0.545
pilM	Legionella pneumophila strain ERS1305867	47.74	100	0.477