Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AOY20_RS05505 Genome accession   NZ_CP012808
Coordinates   1188885..1190666 (-) Length   593 a.a.
NCBI ID   WP_054580936.1    Uniprot ID   -
Organism   Acinetobacter equi strain 114     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1183885..1195666
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AOY20_RS05475 (AOY20_05475) - 1184013..1184336 (+) 324 WP_054580930.1 pyrimidine/purine nucleoside phosphorylase -
  AOY20_RS05480 (AOY20_05480) rlmB 1184466..1185215 (+) 750 WP_054580931.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  AOY20_RS05485 (AOY20_05485) - 1185250..1186137 (+) 888 WP_081403362.1 DMT family transporter -
  AOY20_RS05490 (AOY20_05490) coaE 1186157..1186765 (-) 609 WP_054580933.1 dephospho-CoA kinase -
  AOY20_RS05495 (AOY20_05495) pilD 1186774..1187634 (-) 861 WP_054580934.1 prepilin peptidase Machinery gene
  AOY20_RS05500 (AOY20_05500) pilC 1187634..1188860 (-) 1227 WP_054580935.1 type II secretion system F family protein Machinery gene
  AOY20_RS05505 (AOY20_05505) pilB 1188885..1190666 (-) 1782 WP_054580936.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  AOY20_RS05510 (AOY20_05510) tpiA 1190947..1191738 (+) 792 WP_054580937.1 triose-phosphate isomerase -
  AOY20_RS05515 (AOY20_05515) secG 1191752..1192081 (+) 330 WP_054580938.1 preprotein translocase subunit SecG -
  AOY20_RS05545 (AOY20_05545) rimP 1193016..1193540 (+) 525 WP_054580939.1 ribosome maturation factor RimP -
  AOY20_RS05550 (AOY20_05550) nusA 1193574..1195058 (+) 1485 WP_054580940.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 593 a.a.        Molecular weight: 66830.95 Da        Isoelectric Point: 5.3141

>NTDB_id=157759 AOY20_RS05505 WP_054580936.1 1188885..1190666(-) (pilB) [Acinetobacter equi strain 114]
MQSIPKFTGFIRQLVEKGMIDAEHMLSAIETAKQLDQDIVPYLIQNHHIHPLDIAKAISHEFGDPLFDLDAYDSSLIIKD
SVNDKIITKYKILPIFRRANILYVATANPTNITAIEAIRFSSQMHIEAIIVEYDKLKKLIEQHYSGEEIFNFDDDFDLEI
SDDTQKTTPTEDDILKDESPIVKYVNKLLVDAIRIGASDLHFEPYEKIYRVRYRIDGVLRQIATPPLQLATRLASRIKIM
SQMDISEKRIPQDGRIKLKLSKHKAFDFRVNSLPTLYGEKLVLRILDSSSAMMGIDSLGYEPDQKALFLEALSKPQGMLL
ITGPTGSGKTVSLYTGISILNQEEVNISTVEDPIEINLEGINQVNVNQKVGLTFATTLRSFLRQDPDIIMVGEIRDLETA
EIATTLRSFLRQDPDIIMVGEIRDLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLA
RRLCHLCKRPINIPFQSLLEMGFTEEDIQNPTFQLYDANGCSACHDGYKGRIGIYEVMKMTPEISRLIMEDCNSIQIAEA
SQLSGFQNLRRSGLLKAMQGITSLQEINRITSE

Nucleotide


Download         Length: 1782 bp        

>NTDB_id=157759 AOY20_RS05505 WP_054580936.1 1188885..1190666(-) (pilB) [Acinetobacter equi strain 114]
ATGCAATCTATACCGAAGTTTACCGGTTTTATTCGGCAACTTGTTGAGAAAGGAATGATCGATGCAGAACATATGTTATC
TGCAATTGAAACAGCTAAACAACTAGACCAAGATATCGTTCCTTATTTAATCCAAAACCATCATATTCACCCACTCGATA
TTGCCAAAGCAATTTCTCATGAGTTCGGTGATCCTTTATTTGATTTAGATGCTTATGATTCATCATTAATTATTAAAGAC
TCTGTAAATGATAAAATAATCACTAAATACAAAATCTTGCCTATTTTTAGGCGAGCAAACATTTTATATGTAGCTACAGC
AAATCCAACAAACATTACAGCAATAGAAGCAATTCGCTTTAGCAGCCAAATGCATATTGAGGCGATTATTGTTGAATATG
ACAAACTTAAAAAATTAATAGAACAACATTATAGTGGTGAAGAGATATTTAACTTCGATGATGACTTTGATTTAGAAATC
AGTGATGACACCCAAAAAACCACACCAACTGAAGATGATATTTTAAAAGATGAATCCCCTATTGTTAAATATGTAAATAA
ATTGCTTGTTGATGCAATTCGAATTGGAGCCTCTGATTTACATTTCGAACCTTATGAAAAAATTTATCGTGTTCGATATC
GAATTGATGGTGTTTTAAGGCAAATTGCAACACCACCACTACAACTAGCAACACGCTTGGCTTCACGTATAAAAATCATG
TCCCAAATGGATATTTCAGAAAAACGTATTCCTCAAGATGGTCGAATCAAACTTAAACTTTCTAAACATAAAGCATTTGA
CTTCAGGGTAAATTCGTTACCTACCCTATATGGTGAAAAATTAGTGCTTAGAATACTAGATTCATCTAGTGCAATGATGG
GTATTGATTCTTTAGGTTACGAGCCAGATCAAAAAGCTTTATTTTTGGAAGCACTAAGCAAACCTCAAGGAATGTTGCTA
ATAACGGGCCCAACAGGTTCAGGAAAAACTGTTTCACTCTACACAGGAATCAGTATTCTCAACCAAGAAGAAGTCAATAT
TTCTACTGTAGAAGACCCTATTGAAATCAACCTAGAAGGAATCAATCAAGTCAATGTAAATCAAAAAGTTGGGTTAACGT
TTGCAACTACATTACGCTCCTTTCTACGACAAGATCCCGACATTATTATGGTCGGAGAAATTCGAGATTTAGAAACAGCT
GAAATTGCAACTACATTACGCTCCTTTCTACGACAAGATCCCGACATTATTATGGTCGGAGAAATTCGAGATTTAGAAAC
AGCTGAAATTGCAATTAAAGCTGCCCAAACTGGTCACATGGTGATGTCAACATTACACACTAACAGTGCTCCTGAAACAT
TAACACGCTTAAGAAATATGGGTGTACCTTCATTTAATATTGCAACTTCAGTCAATTTAGTCATTGCACAACGATTAGCA
AGACGGCTTTGCCATCTATGTAAAAGACCAATCAATATTCCATTTCAAAGCTTATTAGAAATGGGGTTTACAGAAGAAGA
TATACAAAATCCGACATTCCAACTCTATGATGCAAATGGTTGCTCTGCTTGCCATGATGGGTATAAAGGTCGAATTGGCA
TTTATGAAGTGATGAAAATGACGCCTGAAATTTCACGACTCATTATGGAAGATTGTAATTCGATACAAATTGCGGAAGCT
TCACAACTATCTGGGTTTCAAAATTTAAGACGCTCTGGGCTACTCAAAGCAATGCAAGGAATTACCTCACTCCAAGAAAT
AAACCGTATTACAAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(187-403)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(14-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

67.395

100

0.676
  pilB Acinetobacter baylyi ADP1

67.005

99.663

0.668
  pilB Legionella pneumophila strain ERS1305867

47.563

100

0.477
  pilF Neisseria gonorrhoeae MS11

45.854

99.663

0.457
  pilB Vibrio parahaemolyticus RIMD 2210633

45.145

98.988

0.447
  pilB Vibrio cholerae strain A1552

48.007

93.086

0.447
  pilB Vibrio campbellii strain DS40M4

44.898

99.157

0.445

Multiple sequence alignment