Detailed information NTG0157757

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AOY20_RS05495
• Genome accession: NZ_CP012808
• Coordinates: 1186774..1187634 (-)
• Length: 286 a.a.
• NCBI ID: WP_054580934.1
• Uniprot ID: A0A0N9VNU2
• Organism: Acinetobacter equi strain 114
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1181774..1192634

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AOY20_RS05470 (AOY20_05470)	recN	1181855..1183513 (-)	1659	WP_054580929.1	DNA repair protein RecN	-
AOY20_RS05475 (AOY20_05475)	-	1184013..1184336 (+)	324	WP_054580930.1	pyrimidine/purine nucleoside phosphorylase	-
AOY20_RS05480 (AOY20_05480)	rlmB	1184466..1185215 (+)	750	WP_054580931.1	23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB	-
AOY20_RS05485 (AOY20_05485)	-	1185250..1186137 (+)	888	WP_081403362.1	DMT family transporter	-
AOY20_RS05490 (AOY20_05490)	coaE	1186157..1186765 (-)	609	WP_054580933.1	dephospho-CoA kinase	-
AOY20_RS05495 (AOY20_05495)	pilD	1186774..1187634 (-)	861	WP_054580934.1	prepilin peptidase	Machinery gene
AOY20_RS05500 (AOY20_05500)	pilC	1187634..1188860 (-)	1227	WP_054580935.1	type II secretion system F family protein	Machinery gene
AOY20_RS05505 (AOY20_05505)	pilB	1188885..1190666 (-)	1782	WP_054580936.1	ATPase, T2SS/T4P/T4SS family	Machinery gene
AOY20_RS05510 (AOY20_05510)	tpiA	1190947..1191738 (+)	792	WP_054580937.1	triose-phosphate isomerase	-
AOY20_RS05515 (AOY20_05515)	secG	1191752..1192081 (+)	330	WP_054580938.1	preprotein translocase subunit SecG	-

Sequence

Protein

• Length: 286 a.a.
• Molecular weight: 32503.86 Da
• Isoelectric Point: 7.6248

>NTDB_id=157757 AOY20_RS05495 WP_054580934.1 1186774..1187634(-) (pilD) [Acinetobacter equi strain 114]
MENFIQYFMTHPTALYIAVGIFSLCIGSFLNVVIYRTPKIMEQEWHQECQLLLHPEQPIIDQKKLTLSHPKSTCPHCHHA
IHWYHNIPVISWMVLLGKCAYCKYKVSWRYPCIEMLTAICALTVVYIYGPSVQMLFGLVLTYFLLALSFIDFDTQLLPDH
LTLPLAGLGLAINSFSLYTSPSAAIWGYIVGFLCLWIVYYIFKLITGKEGMGYGDFKLLAALGAWLGPLYLPLIILLSSF
IGAIIGIVLMKVRKENLPFAFGPYIAIAGWIAFLWGDSIMTFYLSY

Nucleotide

• Length: 861 bp

>NTDB_id=157757 AOY20_RS05495 WP_054580934.1 1186774..1187634(-) (pilD) [Acinetobacter equi strain 114]
ATGGAAAATTTTATTCAGTATTTTATGACACACCCAACTGCACTTTATATTGCAGTTGGGATTTTTAGCTTATGTATTGG
TAGCTTCTTAAACGTTGTGATTTATCGCACACCTAAAATAATGGAACAAGAGTGGCATCAGGAATGTCAGCTTTTATTAC
ATCCGGAACAACCTATAATTGACCAAAAAAAATTAACATTGAGTCATCCTAAGTCTACTTGTCCACATTGTCATCACGCT
ATTCATTGGTATCACAATATTCCTGTAATCAGTTGGATGGTATTACTTGGCAAGTGTGCATATTGCAAATATAAAGTTAG
TTGGCGTTATCCATGTATTGAAATGCTTACAGCCATTTGTGCCTTAACTGTAGTCTATATTTATGGTCCAAGTGTACAAA
TGCTTTTTGGTTTAGTACTGACCTATTTTCTTCTTGCGCTTAGCTTCATTGATTTTGATACACAACTGTTACCTGATCAC
CTCACCCTCCCCTTGGCAGGCTTGGGTTTAGCCATCAATAGTTTTTCACTTTACACATCTCCCAGCGCTGCAATTTGGGG
TTATATTGTTGGTTTTTTATGTCTTTGGATCGTTTATTATATTTTTAAGCTGATTACAGGTAAGGAAGGCATGGGATATG
GTGACTTTAAGCTTCTTGCTGCACTTGGCGCATGGCTAGGTCCTTTATACCTCCCCCTGATTATTCTTCTTTCTTCTTTT
ATTGGTGCTATTATTGGCATAGTTCTAATGAAAGTACGCAAAGAAAATTTGCCTTTCGCATTTGGACCATATATTGCTAT
TGCTGGATGGATCGCATTTTTATGGGGTGATTCAATCATGACATTTTATTTAAGTTACTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (138-247)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-126)

3D structure

• AlphaFold DB: A0A0N9VNU2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Acinetobacter nosocomialis M2	71.579	99.65	0.713
pilD	Acinetobacter baumannii D1279779	71.228	99.65	0.71
pilD	Vibrio cholerae strain A1552	50.699	100	0.507
pilD	Vibrio campbellii strain DS40M4	49.65	100	0.497
pilD	Neisseria gonorrhoeae MS11	45.802	91.608	0.42