Detailed information NTG0157752

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: AOY20_RS05240
• Genome accession: NZ_CP012808
• Coordinates: 1133725..1134783 (+)
• Length: 352 a.a.
• NCBI ID: WP_054580887.1
• Uniprot ID: A0A0N9V6Y5
• Organism: Acinetobacter equi strain 114
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1128725..1139783

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AOY20_RS05225 (AOY20_05225)	mraY	1128967..1130085 (+)	1119	WP_054580884.1	phospho-N-acetylmuramoyl-pentapeptide- transferase	-
AOY20_RS05230 (AOY20_05230)	-	1130152..1130958 (+)	807	WP_054580885.1	putative RNA methyltransferase	-
AOY20_RS05235 (AOY20_05235)	ponA	1131032..1133563 (-)	2532	WP_054580886.1	penicillin-binding protein PBP1a	-
AOY20_RS05240 (AOY20_05240)	pilM	1133725..1134783 (+)	1059	WP_054580887.1	pilus assembly protein PilM	Machinery gene
AOY20_RS05245 (AOY20_05245)	pilN	1134783..1135376 (+)	594	WP_054580888.1	PilN domain-containing protein	Machinery gene
AOY20_RS05250 (AOY20_05250)	pilO	1135454..1136149 (+)	696	WP_054580889.1	type 4a pilus biogenesis protein PilO	Machinery gene
AOY20_RS05255 (AOY20_05255)	pilP	1136152..1136667 (+)	516	WP_054580890.1	pilus assembly protein PilP	Machinery gene
AOY20_RS05260 (AOY20_05260)	pilQ	1136727..1138787 (+)	2061	WP_054580891.1	type IV pilus secretin PilQ	Machinery gene
AOY20_RS05265 (AOY20_05265)	aroK	1138825..1139367 (+)	543	WP_054580892.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39789.43 Da
• Isoelectric Point: 4.9116

>NTDB_id=157752 AOY20_RS05240 WP_054580887.1 1133725..1134783(+) (pilM) [Acinetobacter equi strain 114]
MPRLYNKSKDGVIGVDISSTSVKLLEFSVRNNEYWVENYAVIPLPEGSVIEKNILNTENVGDAIAKALDFTSFRSRYAAI
AIPTSMVITKIIEMDTDMHDDEREVQIRMDAEQYIPFPLDDVSLDFEVLPDRLSLPNRVNVLLVLTRVENVEIRREAIEL
SGLVPKIADVENFAIENAFQLFTDSIPMGTKTIAILDVGHTMTTLSVLQHNKIIYNREQIFGGKQLTNDIQRHYGLSFEE
AERAKKTHVLPDDYDSQVLQPFLESVIQQASRSLQFFFSSSQYHDIDHILLAGGCANIQGLSRLLQQKLNYRVTIANPIL
HMRFSSNVDIQKIKNDAPSLMVASGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=157752 AOY20_RS05240 WP_054580887.1 1133725..1134783(+) (pilM) [Acinetobacter equi strain 114]
GTGCCTAGATTATACAATAAATCTAAAGATGGGGTAATAGGCGTAGATATTAGTTCTACTTCTGTTAAATTATTAGAGTT
CTCTGTAAGAAATAATGAGTATTGGGTTGAAAATTATGCAGTTATTCCTTTACCTGAAGGAAGTGTAATTGAAAAAAATA
TTCTAAATACAGAAAATGTAGGAGATGCAATAGCAAAAGCGCTAGATTTTACGAGTTTTCGATCGCGATATGCGGCAATC
GCAATTCCCACATCAATGGTCATTACTAAAATCATTGAGATGGATACAGACATGCATGATGATGAACGAGAAGTTCAGAT
TCGGATGGATGCTGAGCAATATATTCCATTTCCTTTAGATGATGTTAGTCTTGATTTTGAAGTACTACCAGATCGATTAT
CTTTACCTAATCGAGTGAATGTTCTACTTGTTTTGACCCGTGTAGAAAATGTTGAAATTAGACGAGAAGCGATAGAGCTT
AGTGGTTTAGTTCCTAAAATTGCAGATGTTGAAAACTTTGCAATAGAAAATGCGTTTCAACTTTTTACCGACTCAATTCC
TATGGGAACTAAAACAATTGCTATTTTGGATGTTGGTCATACCATGACGACCTTATCCGTTTTGCAACATAATAAAATTA
TTTATAACCGTGAACAAATTTTTGGTGGAAAACAACTGACGAACGATATTCAAAGACATTATGGATTATCATTTGAAGAA
GCAGAACGAGCCAAAAAAACACACGTATTGCCTGATGATTATGATTCACAGGTGTTACAACCATTTTTAGAGTCTGTTAT
TCAACAAGCATCTAGATCTTTACAGTTTTTCTTTTCATCTTCTCAATATCATGATATTGATCATATTTTATTGGCTGGTG
GATGTGCGAATATTCAAGGACTTTCACGGTTATTACAACAAAAATTAAATTACAGAGTAACGATTGCAAATCCAATATTA
CATATGCGGTTTTCTTCGAATGTAGATATTCAAAAAATTAAAAATGATGCACCATCATTGATGGTCGCTAGTGGTTTAGC
ACTTAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: A0A0N9V6Y5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	70.455	100	0.705
comM	Acinetobacter nosocomialis M2	70.455	100	0.705
comM	Acinetobacter baylyi ADP1	66.193	100	0.662
pilM	Legionella pneumophila strain ERS1305867	40.282	100	0.406