Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   AOY20_RS05250 Genome accession   NZ_CP012808
Coordinates   1135454..1136149 (+) Length   231 a.a.
NCBI ID   WP_054580889.1    Uniprot ID   A0A0N9VNQ2
Organism   Acinetobacter equi strain 114     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1130454..1141149
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AOY20_RS05235 (AOY20_05235) ponA 1131032..1133563 (-) 2532 WP_054580886.1 penicillin-binding protein PBP1a -
  AOY20_RS05240 (AOY20_05240) pilM 1133725..1134783 (+) 1059 WP_054580887.1 pilus assembly protein PilM Machinery gene
  AOY20_RS05245 (AOY20_05245) pilN 1134783..1135376 (+) 594 WP_054580888.1 PilN domain-containing protein Machinery gene
  AOY20_RS05250 (AOY20_05250) pilO 1135454..1136149 (+) 696 WP_054580889.1 type 4a pilus biogenesis protein PilO Machinery gene
  AOY20_RS05255 (AOY20_05255) pilP 1136152..1136667 (+) 516 WP_054580890.1 pilus assembly protein PilP Machinery gene
  AOY20_RS05260 (AOY20_05260) pilQ 1136727..1138787 (+) 2061 WP_054580891.1 type IV pilus secretin PilQ Machinery gene
  AOY20_RS05265 (AOY20_05265) aroK 1138825..1139367 (+) 543 WP_054580892.1 shikimate kinase AroK -
  AOY20_RS05270 (AOY20_05270) aroB 1139387..1140469 (+) 1083 WP_054580893.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 231 a.a.        Molecular weight: 26532.60 Da        Isoelectric Point: 6.7231

>NTDB_id=157754 AOY20_RS05250 WP_054580889.1 1135454..1136149(+) (pilO) [Acinetobacter equi strain 114]
MEKYNNVTPNTTVAKKKKFDFEKFMHQWNTLDPQNYGGWPIAVKMTICCFILLLISILGYLVIIKPKILEIENAQGLERN
LIEEFRKKESTLRNLLQYQQQLQEMQISFNQQLEQLPKETEISGLVEDLNITGINSGLKFNNILLEPEIKQEFIIEQPIS
IEATGDYHAFGTFVSQSAVLPRIVTLHDFVVTTGQNEQKTTAIPRITYSIKAKTYRYVGSLPQSKASTEEK

Nucleotide


Download         Length: 696 bp        

>NTDB_id=157754 AOY20_RS05250 WP_054580889.1 1135454..1136149(+) (pilO) [Acinetobacter equi strain 114]
ATGGAAAAGTATAATAACGTTACTCCAAATACGACAGTTGCAAAAAAGAAGAAGTTTGATTTTGAAAAATTTATGCATCA
ATGGAATACACTAGATCCACAAAATTATGGTGGATGGCCAATTGCGGTCAAAATGACCATTTGTTGCTTTATTCTTTTAT
TGATTTCAATTTTAGGATATCTCGTAATCATCAAACCTAAAATTTTAGAAATTGAAAATGCGCAAGGACTTGAGCGGAAT
TTAATTGAAGAATTTAGAAAGAAAGAATCAACTTTAAGAAATTTATTGCAGTATCAGCAACAATTACAGGAAATGCAGAT
TAGCTTTAACCAACAATTAGAGCAATTGCCTAAAGAAACTGAAATTTCTGGGTTAGTCGAAGACTTAAATATTACAGGTA
TAAATTCAGGATTAAAATTTAATAATATTTTATTAGAGCCAGAAATTAAGCAAGAATTCATTATTGAGCAGCCTATTTCT
ATAGAGGCAACAGGTGATTACCACGCATTTGGAACATTTGTGAGTCAGAGTGCCGTGTTACCTCGTATTGTGACTTTACA
TGATTTTGTTGTGACTACAGGACAAAATGAGCAAAAAACAACAGCCATTCCTCGAATTACTTATTCAATCAAGGCAAAAA
CATATCGCTATGTAGGTAGCCTTCCGCAATCTAAAGCATCTACAGAGGAGAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(71-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0N9VNQ2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

63.679

91.775

0.584
  comO Acinetobacter baylyi ADP1

62.617

92.641

0.58

Multiple sequence alignment