Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   FORC17_RS01620 Genome accession   NZ_CP012739
Coordinates   334556..336340 (+) Length   594 a.a.
NCBI ID   WP_013571034.1    Uniprot ID   A0A2S3R784
Organism   Vibrio vulnificus strain FORC_017     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 329556..341340
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC17_RS01600 (FORC17_0285) pilM 331870..332877 (+) 1008 WP_017419656.1 type IV pilus assembly protein PilM Machinery gene
  FORC17_RS01605 (FORC17_0286) pilN 332867..333457 (+) 591 WP_011079353.1 PilN domain-containing protein Machinery gene
  FORC17_RS01610 (FORC17_0287) pilO 333450..334040 (+) 591 WP_011079354.1 type 4a pilus biogenesis protein PilO Machinery gene
  FORC17_RS01615 (FORC17_0288) pilP 334030..334542 (+) 513 WP_011079355.1 pilus assembly protein PilP Machinery gene
  FORC17_RS01620 (FORC17_0289) pilQ 334556..336340 (+) 1785 WP_013571034.1 type IV pilus secretin PilQ Machinery gene
  FORC17_RS01625 (FORC17_0290) aroK 336542..337060 (+) 519 WP_011079357.1 shikimate kinase AroK -
  FORC17_RS01630 (FORC17_0291) aroB 337093..338184 (+) 1092 WP_011079358.1 3-dehydroquinate synthase -
  FORC17_RS01635 (FORC17_0292) - 338235..339731 (+) 1497 WP_045623943.1 AAA family ATPase -
  FORC17_RS01640 (FORC17_0293) - 339811..340638 (+) 828 WP_039554836.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 594 a.a.        Molecular weight: 64550.65 Da        Isoelectric Point: 4.5316

>NTDB_id=157424 FORC17_RS01620 WP_013571034.1 334556..336340(+) (pilQ) [Vibrio vulnificus strain FORC_017]
MICELMMKNKIQLFPGMISQFVCLLSVCIFTTFANAQSEAENALKSIDFRTNAAKDAIIIVELTTPATVVDLKPVAEGLS
IELINTSVKDEQLYLLDVKDFSTVVESIEVFREVSKTRLVANVSGEFRHDYRLSGRYLEVTISRIKSEQEAREQSVLEKE
GKLISINFQDIPVRNVLQLIADYNGFNLVVSDSVTGNVTLRLDGVPWQQVLDIILQVKGLDKRVEGNVVLIAPQDELDLR
EKQQLEKERLAEELGSLKSEIIKVNFAKASDIAEMINGKESVSMLSERGSVAIDERTNSLLLRELPENIEVIREIINSLD
IPVKQVQIEARIVTVNEGNLDELGVRWGFTSINGSTTVGGSIENNLATIGLYNAGSGSGSGDSGSGGSGSEGGSASIDDF
LNVNLAATSANASSIAFQVAKLGSDLLLDLELSALQRESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATTVSF
KKAVLSLKVTPQITPDNRLVLDLSVTQDRPGEIVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNTTDKVPL
LGDLPLLGALFRRSYENVGKRELLIFVTPKVILQ

Nucleotide


Download         Length: 1785 bp        

>NTDB_id=157424 FORC17_RS01620 WP_013571034.1 334556..336340(+) (pilQ) [Vibrio vulnificus strain FORC_017]
GTGATTTGTGAGTTGATGATGAAAAACAAAATCCAATTGTTTCCGGGAATGATTTCCCAATTCGTCTGCCTGTTGTCTGT
TTGCATCTTTACCACTTTTGCAAACGCACAATCTGAAGCAGAAAATGCGCTCAAGAGCATCGATTTCAGAACCAACGCAG
CCAAAGATGCGATTATCATCGTTGAATTGACAACGCCTGCAACGGTGGTGGATTTGAAACCGGTGGCAGAAGGATTGAGC
ATCGAGTTGATCAATACCTCGGTCAAAGATGAGCAGCTCTATCTGCTCGATGTCAAAGATTTCTCGACCGTGGTTGAAAG
TATCGAAGTATTCAGAGAAGTGTCCAAAACCCGTCTTGTTGCGAATGTTAGTGGTGAATTTCGTCACGACTATCGTCTGT
CTGGACGCTATCTCGAGGTGACAATTTCTCGTATCAAATCGGAACAAGAAGCCCGTGAACAAAGTGTGTTGGAAAAAGAA
GGCAAGTTGATTTCTATCAACTTCCAAGATATTCCAGTGCGTAACGTTTTGCAGCTTATCGCAGACTACAATGGGTTCAA
CCTCGTGGTGTCTGATTCCGTGACTGGTAATGTGACGTTGAGATTGGATGGGGTACCTTGGCAGCAAGTGCTCGATATCA
TTCTTCAAGTGAAAGGTTTAGATAAACGTGTTGAAGGCAATGTTGTGCTCATCGCTCCTCAAGATGAATTGGATTTGCGA
GAGAAGCAGCAGCTTGAGAAAGAGCGTCTGGCGGAAGAACTTGGGTCCCTCAAATCGGAAATTATCAAGGTCAACTTTGC
CAAAGCGTCAGACATCGCTGAGATGATCAATGGCAAAGAGTCGGTGAGCATGCTGTCAGAGCGTGGTAGTGTGGCGATTG
ATGAACGGACAAACTCTCTTTTGTTGCGTGAGTTACCCGAAAACATCGAAGTGATTCGAGAAATTATCAATTCGTTGGAT
ATCCCAGTCAAACAGGTACAAATTGAAGCGCGAATTGTCACGGTGAACGAAGGCAATCTTGATGAGCTTGGTGTGCGTTG
GGGTTTCACCTCCATCAACGGCAGCACAACTGTTGGTGGTTCAATCGAAAACAACCTGGCAACCATTGGTCTCTATAATG
CAGGCAGTGGTAGTGGCAGTGGTGACTCAGGTAGCGGCGGCAGTGGTAGTGAAGGCGGTTCGGCCTCCATTGATGATTTC
CTCAATGTCAATCTTGCTGCGACCTCTGCGAACGCCTCAAGTATTGCTTTCCAAGTGGCTAAATTGGGGTCGGATTTGTT
GTTGGATCTCGAACTCTCTGCACTGCAACGCGAATCAAAAGCGGAAATTATTTCTAGCCCTCGCTTGATCACAACCAATA
AGAAGCCTGCTTATATCGAGCAAGGTACGGAGATCCCTTATCTAGAGTCTTCCTCCAGTGGAGCGACCACGGTTTCATTT
AAGAAAGCCGTGCTTAGCCTCAAAGTGACCCCACAGATCACGCCTGACAATCGTCTTGTGTTGGATTTAAGCGTTACACA
AGATAGACCCGGCGAAATTGTCAAAACAGGCACTGGAGAAGCAGTTGCGATTGATACTCAGCGCATCGGTACACAAGTCT
TGGTCAATAATGGTGAAACGGTAGTGTTAGGCGGTATTTTCCAACACAGCATAACCAATACCACGGACAAGGTTCCTCTG
TTAGGCGATTTACCCTTATTGGGTGCGCTTTTCCGCCGTAGCTATGAAAACGTAGGCAAACGTGAATTGCTAATATTTGT
CACACCTAAGGTTATCTTGCAGTAG

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(434-592)

Bacterial type II/III secretion system short domain (Secretin_N)

(260-325)

Secretin and TonB N terminus short domain (STN)

(186-234)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S3R784

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

77.797

99.327

0.773
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

73.345

99.158

0.727
  pilQ Vibrio cholerae strain A1552

73.345

99.158

0.727
  pilQ Legionella pneumophila strain ERS1305867

41.156

96.128

0.396
  pilQ Legionella pneumophila strain Lp02

41.429

94.276

0.391
  pilQ Pseudomonas aeruginosa PAK

39.539

94.949

0.375
  comQ Acinetobacter baylyi ADP1

37.162

99.663

0.37

Multiple sequence alignment