Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   FORC17_RS01600 Genome accession   NZ_CP012739
Coordinates   331870..332877 (+) Length   335 a.a.
NCBI ID   WP_017419656.1    Uniprot ID   A0A087I1V6
Organism   Vibrio vulnificus strain FORC_017     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 326870..337877
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC17_RS01585 - 327701..327973 (-) 273 WP_011079349.1 DUF3624 domain-containing protein -
  FORC17_RS01590 (FORC17_0283) oxyR 328247..329146 (+) 900 WP_011079350.1 DNA-binding transcriptional regulator OxyR -
  FORC17_RS01595 (FORC17_0284) - 329217..331730 (-) 2514 WP_065090047.1 penicillin-binding protein 1A -
  FORC17_RS01600 (FORC17_0285) pilM 331870..332877 (+) 1008 WP_017419656.1 type IV pilus assembly protein PilM Machinery gene
  FORC17_RS01605 (FORC17_0286) pilN 332867..333457 (+) 591 WP_011079353.1 PilN domain-containing protein Machinery gene
  FORC17_RS01610 (FORC17_0287) pilO 333450..334040 (+) 591 WP_011079354.1 type 4a pilus biogenesis protein PilO Machinery gene
  FORC17_RS01615 (FORC17_0288) pilP 334030..334542 (+) 513 WP_011079355.1 pilus assembly protein PilP Machinery gene
  FORC17_RS01620 (FORC17_0289) pilQ 334556..336340 (+) 1785 WP_013571034.1 type IV pilus secretin PilQ Machinery gene
  FORC17_RS01625 (FORC17_0290) aroK 336542..337060 (+) 519 WP_011079357.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 335 a.a.        Molecular weight: 37654.05 Da        Isoelectric Point: 6.3873

>NTDB_id=157420 FORC17_RS01600 WP_017419656.1 331870..332877(+) (pilM) [Vibrio vulnificus strain FORC_017]
MGKPFVTGIDIGHRSLKAVILKPYGEHYALLGYKEIIIKDGIFSDNHTLNHQEIVNSLKELKKELPLFRYQVSLAVPDSS
VISKMLSIDAELDGREREFAILQAFAHQSPFPVDELSIDYVQLVEETSKTNHSYQVYATKKEVVESRVRVAEEVGLKPVL
VDVHAHSLCNILTLAAERHPDKASYCLLDVGYDTTSLVMSRRNEPPLVKEFAYGVSHLNASIGDDLTSPDAHEKTDIFNK
ELTERVRRQLQLFQSMDSQSKVNGLWICGEGAITPLLSETLQRKLSLECEVLNPLALFEQKTAKRKRRIVDWHHFSVAAG
LAIGGIQWLEDSHAA

Nucleotide


Download         Length: 1008 bp        

>NTDB_id=157420 FORC17_RS01600 WP_017419656.1 331870..332877(+) (pilM) [Vibrio vulnificus strain FORC_017]
ATGGGTAAGCCGTTCGTTACCGGAATTGATATTGGTCATCGTAGCCTCAAGGCTGTCATTCTGAAACCTTATGGAGAGCA
CTACGCCCTGTTAGGGTACAAAGAAATAATCATTAAAGACGGTATTTTCTCAGATAACCACACTCTGAATCATCAAGAAA
TTGTCAATAGCCTGAAAGAACTGAAGAAGGAACTGCCTCTTTTCCGTTATCAGGTGTCATTGGCGGTGCCTGATAGTTCA
GTGATTAGTAAGATGTTATCAATCGATGCGGAGTTGGATGGTCGAGAGCGAGAATTCGCTATCCTTCAGGCGTTTGCACA
TCAGTCGCCATTTCCTGTCGATGAATTATCGATTGATTATGTCCAATTGGTCGAAGAAACCAGCAAAACGAATCATAGCT
ATCAAGTTTACGCCACCAAAAAAGAGGTGGTGGAAAGCCGTGTGCGTGTCGCAGAAGAAGTGGGACTGAAACCCGTGCTG
GTAGATGTGCATGCGCATAGCCTGTGCAACATCTTAACGTTAGCGGCAGAGCGCCATCCAGATAAAGCCAGCTATTGCTT
GTTGGATGTGGGTTATGACACCACCTCTTTGGTGATGTCGCGTCGCAACGAACCGCCTTTAGTTAAAGAATTTGCCTATG
GGGTGTCTCACCTCAATGCGTCGATAGGCGACGATCTGACTTCTCCTGATGCTCATGAGAAAACCGACATCTTTAATAAA
GAACTGACAGAGCGAGTTCGTCGGCAATTGCAGCTGTTCCAATCTATGGATAGCCAAAGCAAAGTGAATGGCCTCTGGAT
CTGCGGTGAAGGCGCGATTACTCCGTTGTTGAGTGAAACGTTGCAGAGAAAGCTGAGTTTGGAGTGCGAAGTCCTTAATC
CTCTGGCTCTGTTTGAGCAAAAAACGGCGAAAAGAAAGCGCCGTATTGTTGATTGGCACCATTTTTCGGTGGCAGCGGGA
TTAGCGATTGGTGGCATTCAGTGGCTGGAGGATAGCCATGCAGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-323)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A087I1V6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

53.134

100

0.531
  pilM Vibrio cholerae strain A1552

48.974

100

0.499

Multiple sequence alignment