Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AOC03_RS06770 Genome accession   NZ_CP012678
Coordinates   1564301..1566019 (-) Length   572 a.a.
NCBI ID   WP_062534456.1    Uniprot ID   -
Organism   Psychrobacter urativorans strain R310.10B     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1559301..1571019
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AOC03_RS06745 (AOC03_06730) - 1559977..1560312 (+) 336 WP_062534446.1 pyrimidine/purine nucleoside phosphorylase -
  AOC03_RS06750 (AOC03_06735) rlmB 1560410..1561180 (+) 771 WP_062534447.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  AOC03_RS06755 (AOC03_06740) coaE 1561143..1561844 (-) 702 WP_062534449.1 dephospho-CoA kinase -
  AOC03_RS06760 (AOC03_06745) pilD 1561938..1562837 (-) 900 WP_062534451.1 prepilin peptidase Machinery gene
  AOC03_RS06765 (AOC03_06750) pilC 1562874..1564097 (-) 1224 WP_062534453.1 type II secretion system F family protein Machinery gene
  AOC03_RS06770 (AOC03_06755) pilB 1564301..1566019 (-) 1719 WP_062534456.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AOC03_RS06775 (AOC03_06760) tpiA 1566382..1567203 (+) 822 WP_062534458.1 triose-phosphate isomerase -
  AOC03_RS06780 (AOC03_06765) secG 1567415..1567723 (+) 309 WP_062534460.1 preprotein translocase subunit SecG -
  AOC03_RS06795 (AOC03_06780) rimP 1568660..1569160 (+) 501 WP_062534462.1 ribosome maturation factor RimP -
  AOC03_RS06800 (AOC03_06785) nusA 1569309..1570793 (+) 1485 WP_062534463.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 63416.90 Da        Isoelectric Point: 5.4906

>NTDB_id=156274 AOC03_RS06770 WP_062534456.1 1564301..1566019(-) (pilB) [Psychrobacter urativorans strain R310.10B]
MSSTSSNFGGLPQQLINKGIVSEAFMKTALLESQQQQVGLVSYLVANKMADAYALAQLLSKAFDDPFFDLNVLSNDNIPK
DLVDEKIIRKFNALPIFKRGQRLFVALSDPTRIDAIDAIAFNSRLSIETVVVEDDKLKKRIDSLYADTMQNFGSFADSDL
KVGFNDDDNDSDNEGETKLSDNVEEAPVVKFVNKMLVDAIRMGASDLHFEPYEKSYRVRFRVDGVMQKMSNPPVQLAGKI
AARLKVMSQMDISERRLPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKIVLRILDPSSAMLGIEALGYEPDQKEMFLEALH
KPQGMLLITGPTGSGKTVSLYTGLNILNTEETNISTAEDPVEINLEGINQVNVNAKVGLTFSNALKSFLRQDPDIVMVGE
IRDLETAEIAIKAAQTGHMVLSTLHTNSAPETLTRLRNMGVASFNIATSVNLVIAQRLARRLCKNCKKPITIPHKSLLEL
GFNEADLDNPENIIYEPVGCSECREGYKGRVGIYEVMKVSKDISRIIMEDGNAINIKDAALKEGFRDLRRSGILKVLQGV
TSIQEMMRVTSE

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=156274 AOC03_RS06770 WP_062534456.1 1564301..1566019(-) (pilB) [Psychrobacter urativorans strain R310.10B]
ATGTCTAGCACAAGCAGTAATTTTGGTGGTCTGCCGCAGCAATTGATTAACAAAGGCATTGTTAGCGAAGCATTTATGAA
AACGGCACTGCTTGAATCACAGCAACAGCAGGTCGGACTGGTGTCGTATTTGGTAGCCAATAAAATGGCGGATGCTTATG
CGTTGGCACAGCTGCTGTCAAAAGCATTCGACGATCCATTTTTTGATCTGAATGTGCTGAGTAATGACAATATACCAAAA
GACTTGGTTGATGAAAAAATCATCCGTAAGTTTAATGCACTGCCCATCTTTAAGCGTGGGCAGCGCCTGTTTGTAGCACT
CAGTGATCCGACACGTATCGATGCGATTGATGCCATCGCCTTTAATTCACGACTGTCTATTGAAACCGTCGTCGTCGAAG
ACGATAAACTTAAAAAGCGTATCGATAGTCTTTATGCAGATACCATGCAAAATTTTGGTAGTTTTGCCGATAGTGACTTA
AAAGTTGGTTTTAATGATGACGACAATGATAGCGACAATGAGGGCGAAACCAAACTTAGCGATAACGTCGAGGAAGCGCC
AGTTGTCAAATTCGTCAATAAAATGCTGGTGGATGCGATTCGTATGGGTGCATCTGACTTACATTTTGAGCCGTATGAGA
AATCTTACCGAGTGCGCTTTCGGGTGGATGGGGTCATGCAAAAAATGTCTAATCCGCCGGTACAACTGGCGGGCAAAATC
GCGGCACGTCTTAAAGTCATGTCACAGATGGATATCTCAGAACGCCGTTTGCCACAAGATGGACGTATTAAACTCAAATT
ATCTAAAAATAAAGCCATCGACTTTCGAGTAAACTCGTTACCGACACTGTTTGGCGAAAAAATTGTCCTGCGTATTTTGG
ATCCCTCCTCTGCGATGCTCGGTATTGAAGCCTTAGGCTACGAGCCTGATCAAAAAGAGATGTTCTTAGAAGCATTGCAT
AAGCCACAAGGGATGCTGTTAATCACGGGTCCTACTGGTTCTGGTAAAACAGTGTCTTTATATACCGGGCTTAATATTCT
TAATACCGAAGAAACCAATATATCTACTGCTGAAGATCCTGTCGAGATTAATCTTGAAGGTATTAATCAGGTCAACGTGA
ACGCAAAAGTGGGGCTGACCTTCTCGAACGCCCTCAAATCATTTTTACGCCAAGATCCCGATATTGTGATGGTGGGTGAG
ATTCGTGACCTTGAAACCGCCGAAATTGCTATTAAAGCTGCGCAGACCGGACACATGGTACTGTCAACGCTACACACTAA
CTCCGCACCTGAAACCTTAACTCGTCTGCGTAATATGGGTGTGGCGTCATTTAATATTGCCACGTCAGTGAACTTAGTGA
TTGCGCAGCGCTTGGCACGACGCTTATGCAAAAACTGCAAAAAGCCCATCACCATTCCCCATAAAAGTTTACTAGAACTT
GGCTTTAATGAAGCGGATTTAGATAACCCAGAGAACATTATTTATGAACCTGTCGGCTGTAGTGAATGTCGTGAAGGCTA
TAAAGGTCGAGTGGGTATTTATGAGGTGATGAAAGTCAGTAAAGATATTTCACGTATTATTATGGAAGATGGTAACGCTA
TTAATATTAAAGATGCGGCGCTCAAAGAAGGCTTTCGTGATTTGCGGCGCTCCGGTATTTTAAAAGTATTACAAGGCGTG
ACCAGTATTCAAGAGATGATGCGCGTTACCTCTGAGTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-148)

Type II/IV secretion system protein (T2SSE)

(194-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

67.535

100

0.68
  pilB Acinetobacter baylyi ADP1

63.509

99.65

0.633
  pilB Legionella pneumophila strain ERS1305867

53.889

94.406

0.509
  pilF Neisseria gonorrhoeae MS11

53.089

90.559

0.481
  pilB Vibrio parahaemolyticus RIMD 2210633

48.077

100

0.481
  pilB Vibrio cholerae strain A1552

49.016

97.727

0.479
  pilB Vibrio campbellii strain DS40M4

48.115

97.378

0.469

Multiple sequence alignment