Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AN158_RS10355 Genome accession   NZ_CP012587
Coordinates   2238414..2240126 (-) Length   570 a.a.
NCBI ID   WP_001274987.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain CA-17     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2233414..2245126
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AN158_RS10325 (AN158_10320) - 2233576..2233902 (+) 327 WP_000099685.1 pyrimidine/purine nucleoside phosphorylase -
  AN158_RS10330 (AN158_10325) rlmB 2234004..2234753 (+) 750 WP_069370670.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  AN158_RS10335 (AN158_10330) - 2234789..2235703 (+) 915 WP_000075014.1 DMT family transporter -
  AN158_RS10340 (AN158_10335) coaE 2235700..2236296 (-) 597 WP_069370671.1 dephospho-CoA kinase -
  AN158_RS17920 (AN158_10340) pilD 2236298..2237158 (-) 861 WP_069370672.1 prepilin peptidase Machinery gene
  AN158_RS10350 (AN158_10345) pilC 2237158..2238384 (-) 1227 WP_000279218.1 type II secretion system F family protein Machinery gene
  AN158_RS10355 (AN158_10350) pilB 2238414..2240126 (-) 1713 WP_001274987.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AN158_RS10360 (AN158_10355) tpiA 2240417..2241211 (+) 795 WP_000016939.1 triose-phosphate isomerase -
  AN158_RS10365 (AN158_10360) secG 2241224..2241553 (+) 330 WP_000555570.1 preprotein translocase subunit SecG -
  AN158_RS10385 (AN158_10380) rimP 2242195..2242719 (+) 525 WP_000777730.1 ribosome maturation factor RimP -
  AN158_RS10390 (AN158_10385) nusA 2242757..2244241 (+) 1485 WP_000532248.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63355.71 Da        Isoelectric Point: 5.2391

>NTDB_id=155573 AN158_RS10355 WP_001274987.1 2238414..2240126(-) (pilB) [Acinetobacter baumannii strain CA-17]
MSALHTSPKFTGFFRRLVEEKHVSAATMQTALDAAKRAKQDTVAYLIEEVHLSPSLLAETISAEFAEPYFDLDVYDTSQI
PKDLVDQKLILKHRILPLIQRGQILYVATSNPSNIEAIDAIRFNSKLLVEPVIVEHHKLEKVLGQHFAEESSFDFNDEEF
DLDVNLDGPTAQEDEEEAPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKSKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEDQKALFMEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFAAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKIPADIPKQSLLEMGF
TEQDLAHPDFRVFQPVGCPECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=155573 AN158_RS10355 WP_001274987.1 2238414..2240126(-) (pilB) [Acinetobacter baumannii strain CA-17]
ATGTCAGCATTACATACATCTCCAAAATTTACGGGGTTTTTTCGACGTTTAGTCGAAGAAAAGCATGTGTCGGCAGCGAC
CATGCAAACTGCATTAGATGCAGCGAAAAGAGCTAAGCAGGACACAGTCGCATATTTAATTGAAGAGGTTCATCTCTCCC
CTTCTTTATTGGCTGAAACAATTTCTGCTGAGTTTGCTGAACCATATTTCGATCTGGATGTTTATGACACCTCTCAGATT
CCTAAAGATCTGGTCGATCAGAAACTGATTTTAAAACATCGAATTTTGCCGCTCATTCAAAGAGGGCAAATTTTATATGT
TGCGACCAGTAATCCAAGCAATATCGAAGCGATTGATGCAATTCGTTTTAATAGTAAATTGCTGGTTGAGCCTGTAATTG
TGGAGCACCATAAACTCGAAAAAGTTTTAGGCCAGCATTTTGCAGAAGAAAGCAGCTTTGACTTCAATGATGAAGAGTTT
GATCTCGATGTTAATCTTGATGGTCCTACCGCTCAAGAAGATGAAGAAGAAGCTCCTCAAGGCGATGAAGCCCCGATTGT
TAAATATATTAATAAGTTATTAATTGATGCCATTCGTATGGGTGCTTCAGACTTACATTTTGAACCGTACGAAAAATCCT
ATCGGGTGCGCTATCGTGTCGATGGCGTGTTACGTCAAATTGCCAATCCCCCTCTACAGCTTGCAAACCGCTTGGCATCA
CGCTTAAAAGTAATGTCTCAAATGGACATTTCTGAAAAACGCGTCCCACAAGATGGTCGTATCAAGCTCAAGCTGTCAAA
AAGCAAAGCGATTGATTTTCGTGTGAACTCCCTCCCGACTTTGTTTGGTGAAAAGTTAGTTCTACGTATTCTTGACCCAT
CAAGCGCAATGCTAGGAATTGATGCACTAGGATATGAAGAAGATCAAAAAGCTTTGTTTATGGAGGCATTAGATAAACCT
CAGGGCATGCTTTTAATTACCGGGCCAACAGGTTCTGGTAAAACCGTATCGTTATATACAGGTTTAAATATTTTAAATAC
TGAAAGCTCCAATATTTCTACTGCCGAAGACCCGGTAGAAATTAACCTGGAAGGAATTAATCAAGTTAACGTAAACCCCA
AGGTAGGGCTTACTTTTGCGGCCGCACTTAAATCATTTTTACGACAAGACCCTGACATTATTATGGTGGGTGAGATTCGT
GACTTGGAAACAGCAGAAATTGCGATTAAGGCGGCTCAAACCGGCCACATGGTGATGTCAACACTACATACTAATAGTGC
TCCGGAAACATTGACCCGTCTACGCAATATGGGGGTTCCTTCTTTTAATATTGCAACATCAGTAAACCTAGTTATTGCAC
AGCGTTTAGCACGTCGTCTTTGTTCTCAATGCAAAATACCTGCTGATATTCCTAAACAAAGCTTATTAGAAATGGGCTTT
ACCGAACAAGATTTAGCGCACCCAGATTTTCGGGTTTTTCAGCCCGTAGGTTGCCCTGAATGTCGTGAAGGCTATAAAGG
CCGTGTCGGTATTTATGAGGTAATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAACGCTTTAGAAA
TCGCAGCTGCTTCTGAAAAATTGGGGTTTAATAATCTACGTCGTTCAGGTTTAAAAAAGGTGATGCAAGGGGTGACCTCT
TTACAAGAAGTCAACCGTGTCACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

99.825

100

0.998
  pilB Acinetobacter baylyi ADP1

79.825

100

0.798
  pilB Legionella pneumophila strain ERS1305867

50.794

99.474

0.505
  pilF Neisseria gonorrhoeae MS11

50.356

98.596

0.496
  pilB Vibrio parahaemolyticus RIMD 2210633

51.698

92.982

0.481
  pilB Vibrio campbellii strain DS40M4

50.867

91.053

0.463
  pilB Vibrio cholerae strain A1552

50.29

90.702

0.456
  pilB/pilB1 Synechocystis sp. PCC 6803

35.179

100

0.379
  pilF Thermus thermophilus HB27

37.011

98.596

0.365

Multiple sequence alignment