Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   A8V30_RS00560 Genome accession   NZ_CP015846
Coordinates   120199..121584 (-) Length   461 a.a.
NCBI ID   WP_001025184.1    Uniprot ID   -
Organism   Escherichia coli O157:H7 strain FRIK2069     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 115199..126584
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A8V30_RS00530 (A8V30_00535) mutT 115645..116043 (+) 399 WP_000736023.1 8-oxo-dGTP diphosphatase MutT -
  A8V30_RS00535 (A8V30_00545) yacG 116134..116331 (-) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  A8V30_RS00540 (A8V30_00550) zapD 116341..117084 (-) 744 WP_001194728.1 cell division protein ZapD -
  A8V30_RS00545 (A8V30_00555) coaE 117084..117704 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  A8V30_RS31620 - 117729..117773 (+) 45 WP_120795372.1 protein YacM -
  A8V30_RS00550 (A8V30_00560) guaC 117929..118972 (+) 1044 WP_001217330.1 GMP reductase -
  A8V30_RS00555 (A8V30_00565) hofC 119007..120209 (-) 1203 WP_000157271.1 protein transport protein HofC -
  A8V30_RS00560 (A8V30_00570) pilB 120199..121584 (-) 1386 WP_001025184.1 type II secretion system protein GspE Machinery gene
  A8V30_RS00565 (A8V30_00575) ppdD 121594..122034 (-) 441 WP_000360900.1 prepilin peptidase-dependent pilin -
  A8V30_RS00575 (A8V30_00585) nadC 122237..123130 (-) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  A8V30_RS00580 (A8V30_00590) ampD 123218..123769 (+) 552 WP_000923722.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  A8V30_RS00585 (A8V30_00595) ampE 123766..124620 (+) 855 WP_000172008.1 beta-lactamase regulator AmpE -
  A8V30_RS00590 (A8V30_00600) aroP 124663..126036 (-) 1374 WP_010904500.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50594.41 Da        Isoelectric Point: 6.8050

>NTDB_id=155167 A8V30_RS00560 WP_001025184.1 120199..121584(-) (pilB) [Escherichia coli O157:H7 strain FRIK2069]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKMVLRLLQQVSQALDVNTLGMQPLQLAGFAHALQQPQGLVLVTGPTGSGKTVTLYSALQKLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPVNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=155167 A8V30_RS00560 WP_001025184.1 120199..121584(-) (pilB) [Escherichia coli O157:H7 strain FRIK2069]
ATGAATATTCCACAGCTCACGGCCCTGTGCCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAAGTGGTTCATGT
TGCGGTGGTCGATGCCCCCTCACATGAGTTGCTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGATGGTATTAAGGTTGTTACAGCA
GGTGAGCCAGGCACTGGATGTTAACACGCTGGGAATGCAGCCGTTACAACTGGCGGGCTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACCGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAAAGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCCGGACTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACGGCAGAAATTGCCATTAAAGCCGCGCAAACCGGTCACCTGGTGTTGTCTACCCTACACACTAATTCC
ACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGGGTCGCCCGCTGGATGCTATCATCGGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGCAAACTTTGCCCACATTGTCGCCGGCAGCAAGGGGAGCCCATCCACATTCCAGTCAATGTATGGC
CGTCGCCGCTGCCCCACTGGCAAGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGCACTGCCTTATTTGAA
GTTCTGCCCATAACACCGGTCATACGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCCCGACA
GGCGGGTATGCGAACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TATTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(88-353)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

49.74

83.297

0.414
  pilB Glaesserella parasuis strain SC1401

41.253

100

0.414
  pilB Acinetobacter baylyi ADP1

40.385

100

0.41
  pilB Vibrio campbellii strain DS40M4

48.32

83.948

0.406
  pilB Vibrio cholerae strain A1552

46.465

85.9

0.399
  pilB Vibrio parahaemolyticus RIMD 2210633

46.632

83.731

0.39
  pilB Haemophilus influenzae 86-028NP

44.961

83.948

0.377
  pilB Acinetobacter baumannii D1279779

43.909

85.466

0.375
  pilB Haemophilus influenzae Rd KW20

44.444

83.948

0.373
  pilF Neisseria gonorrhoeae MS11

43.896

83.514

0.367
  pilF Thermus thermophilus HB27

40.831

88.72

0.362