Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   A7319_RS10695 Genome accession   NZ_CP015638
Coordinates   2355924..2357342 (+) Length   472 a.a.
NCBI ID   WP_024074814.1    Uniprot ID   A0ACA7P5Z9
Organism   Pseudomonas fluorescens strain L111     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2350924..2362342
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A7319_RS10660 (A7319_10650) gspL 2350935..2352005 (-) 1071 WP_024074811.1 type II secretion system protein GspL -
  A7319_RS10665 (A7319_10655) gspK 2352002..2352880 (-) 879 WP_024074810.1 type II secretion system minor pseudopilin GspK -
  A7319_RS10670 (A7319_10660) gspG 2352889..2353338 (-) 450 WP_024074809.1 type II secretion system major pseudopilin GspG -
  A7319_RS10675 (A7319_10665) gspI 2353487..2353873 (+) 387 WP_024074808.1 type II secretion system minor pseudopilin GspI -
  A7319_RS10680 (A7319_10670) - 2353860..2354291 (-) 432 WP_024074807.1 type II secretion system protein N -
  A7319_RS10685 (A7319_10675) gspH 2354385..2354837 (+) 453 WP_024074806.1 type II secretion system minor pseudopilin GspH -
  A7319_RS10690 (A7319_10680) - 2354834..2355436 (+) 603 WP_024074805.1 type II secretion system protein GspJ -
  A7319_RS10695 (A7319_10685) pilB 2355924..2357342 (+) 1419 WP_024074814.1 type II secretion system ATPase GspE Machinery gene
  A7319_RS10700 (A7319_10690) gspF 2357342..2358553 (+) 1212 WP_024074815.1 type II secretion system inner membrane protein GspF -
  A7319_RS10705 (A7319_10695) - 2358965..2360167 (+) 1203 WP_024074816.1 substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 472 a.a.        Molecular weight: 51581.14 Da        Isoelectric Point: 5.6411

>NTDB_id=154338 A7319_RS10695 WP_024074814.1 2355924..2357342(+) (pilB) [Pseudomonas fluorescens strain L111]
MSLLPYAWAKSQRILLRPSEQGMLLTVCPSTPGWSISEVHRQFGQARLEQVRDDELDGLLASAYADTGSAAAVVGAAENE
VDLDRLMQDMPEITDLLDTQDGAPVIRMINALLTQAARDEASDIHIEPYETHSVVRYRVDGTLRDVVSPRKALHGALVSR
IKIMAQLDIAEKRLPQDGRIALRVAGRPIDIRVSTVPTGHGERVVMRLLDKQAGRLQLETLGMEPQLLARLDTLIRQPHG
IVLVTGPTGSGKTTSLYAALARLDASTSNILTVEDPVEYDLPGISQIQVNAKIDMTFGLALRAILRQDPDIIMIGEIRDL
ETAQIAVQASLTGHLVLATLHTNDAVSAVNRLIDMGVEPFLLASSLLGVLAQRLVRRLCPHCKQEDPAAPGTWRPVGCAQ
CNQIGYSGRTGIHELFCVDDDIRSLIHQGAGEQDLRVAARRAGMFSMREDGERWVRSGATAPEEILRVTRDA

Nucleotide


Download         Length: 1419 bp        

>NTDB_id=154338 A7319_RS10695 WP_024074814.1 2355924..2357342(+) (pilB) [Pseudomonas fluorescens strain L111]
ATGAGCCTTCTGCCCTACGCCTGGGCCAAATCCCAACGCATCCTCCTGCGCCCCAGCGAGCAGGGCATGCTGCTGACCGT
CTGCCCCTCAACCCCCGGCTGGTCCATCAGCGAAGTCCATCGCCAGTTCGGCCAGGCCCGCCTCGAGCAAGTGCGCGATG
ACGAACTCGACGGCCTGCTCGCCAGCGCCTATGCCGATACAGGCAGCGCCGCCGCTGTGGTCGGCGCTGCCGAAAACGAA
GTCGACCTCGACCGCCTGATGCAGGACATGCCCGAGATCACCGACCTGCTCGATACCCAGGACGGTGCGCCGGTGATCCG
CATGATCAACGCCTTGCTGACCCAGGCCGCGCGGGACGAGGCCAGTGATATTCATATCGAGCCGTATGAAACCCATTCCG
TGGTGCGCTACCGCGTCGACGGCACCCTGCGTGACGTGGTGTCGCCGCGCAAGGCGTTGCATGGCGCGCTGGTGTCGCGC
ATCAAGATCATGGCGCAGCTCGACATCGCCGAAAAACGCTTGCCCCAGGACGGCCGTATTGCCCTGCGCGTGGCCGGGCG
GCCGATTGATATTCGTGTGTCGACCGTGCCCACCGGGCATGGCGAACGCGTTGTGATGCGCTTGCTGGACAAGCAGGCCG
GGCGTCTGCAATTGGAGACCCTGGGCATGGAGCCGCAGTTGCTCGCGCGCCTCGACACCTTGATTCGCCAGCCCCATGGC
ATCGTGCTGGTCACCGGGCCCACCGGCAGCGGCAAGACCACCAGCCTGTACGCGGCCCTGGCGCGGCTGGATGCGAGCAC
CAGCAATATCCTCACCGTCGAAGACCCGGTGGAGTACGACCTGCCGGGTATCAGCCAGATCCAGGTCAACGCCAAGATCG
ACATGACCTTCGGCCTCGCGCTGCGGGCGATCCTGCGCCAGGACCCGGACATCATCATGATCGGCGAAATCCGCGACCTG
GAGACCGCCCAAATCGCCGTGCAGGCCTCGCTCACCGGCCACCTGGTGCTCGCCACCTTGCACACCAACGACGCGGTGTC
GGCGGTCAACCGCCTGATCGACATGGGCGTCGAACCGTTTTTGCTGGCCTCGTCACTGCTGGGCGTATTGGCCCAGCGCC
TGGTGCGGCGCCTGTGCCCGCATTGCAAGCAGGAAGACCCCGCCGCGCCTGGCACCTGGCGCCCGGTGGGCTGCGCGCAG
TGCAACCAGATCGGCTACAGCGGGCGTACCGGCATTCATGAGTTGTTTTGCGTCGACGACGATATCCGCAGCCTGATCCA
CCAAGGCGCCGGCGAGCAGGATTTGCGCGTGGCCGCACGCCGTGCGGGGATGTTCAGCATGCGTGAAGACGGCGAGCGCT
GGGTGCGCAGCGGCGCCACCGCGCCCGAAGAAATCCTGCGCGTGACACGGGACGCCTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(111-377)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

44.492

100

0.445
  pilB Vibrio campbellii strain DS40M4

45.638

94.703

0.432
  pilF Neisseria gonorrhoeae MS11

45.125

93.432

0.422
  pilB Vibrio parahaemolyticus RIMD 2210633

46.359

87.288

0.405
  pilB/pilB1 Synechocystis sp. PCC 6803

40.685

98.941

0.403
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.436

82.627

0.392
  pilB Legionella pneumophila strain ERS1305867

44.878

86.864

0.39
  pilF Thermus thermophilus HB27

48.404

79.661

0.386
  pilB Acinetobacter baylyi ADP1

46.392

82.203

0.381
  pilB Acinetobacter baumannii D1279779

45.897

82.627

0.379
  ctsE Campylobacter jejuni subsp. jejuni 81-176

39.627

90.89

0.36