Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ADJ80_RS00025 Genome accession   NZ_CP012067
Coordinates   3521..4930 (+) Length   469 a.a.
NCBI ID   WP_005704570.1    Uniprot ID   A0A3S4QTD0
Organism   Aggregatibacter aphrophilus strain W10433     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1..9930
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ADJ80_RS00010 (ADJ80_00010) nanQ 1425..1895 (+) 471 WP_050692437.1 N-acetylneuraminate anomerase -
  ADJ80_RS00015 (ADJ80_00015) ampD 2368..2922 (-) 555 WP_050694142.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ADJ80_RS00020 (ADJ80_00020) pilA 3047..3496 (+) 450 WP_050692438.1 pilin Machinery gene
  ADJ80_RS00025 (ADJ80_00025) pilB 3521..4930 (+) 1410 WP_005704570.1 GspE/PulE family protein Machinery gene
  ADJ80_RS00030 (ADJ80_00030) pilC 4923..6161 (+) 1239 WP_050692439.1 type II secretion system F family protein Machinery gene
  ADJ80_RS00035 (ADJ80_00035) - 6158..6850 (+) 693 WP_050692440.1 prepilin peptidase -
  ADJ80_RS00040 (ADJ80_00040) coaE 6903..7523 (+) 621 WP_050692441.1 dephospho-CoA kinase -
  ADJ80_RS00045 (ADJ80_00045) yacG 7516..7728 (+) 213 WP_005701268.1 DNA gyrase inhibitor YacG -
  ADJ80_RS00050 (ADJ80_00050) - 7728..7994 (+) 267 WP_005704566.1 GNAT family N-acetyltransferase -
  ADJ80_RS00055 (ADJ80_00055) - 8362..9735 (+) 1374 WP_050692442.1 alanine/glycine:cation symporter family protein -

Sequence

Protein


Download         Length: 469 a.a.        Molecular weight: 52968.29 Da        Isoelectric Point: 5.8266

>NTDB_id=151012 ADJ80_RS00025 WP_005704570.1 3521..4930(+) (pilB) [Aggregatibacter aphrophilus strain W10433]
MQHSMEQPYSICSQQGDIFTITPELWQQNQQQQTVLLRYLALPLKEEPQKLWLGLDSLTNLAACEAFSFLTGKNIEPVLI
ESAVLKTALQDLAPHREKLDENQPLFYSVTPQEQQEQSSDEPTIQLLNQIFENATTKKASDIHLEPQADFLQVRFRIDGV
LQVQNTIAQTLANRLISRLKLLAKLDISETRLPQDGRFQFKTTFSDILDFRLSTLATHFGEKAVLRLQQNRPVQLAFSEL
GMTEQQQQTFRHALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIQLPGIIQSQVNPQIGLDFSRLL
RTFLRQDPDIIMLGEIRDEESAAMALRAAQTGHLVLSTLHTNDAASAISRLQQLGIQQHEIDSSLLLVIAQRLVRKRCQK
CGENSTRFCDCHQGYKGRIGVYQFLQPDVSQNQSYQLDFQNLYASALEKVKSQVTDLAEVQRVLGQPNA

Nucleotide


Download         Length: 1410 bp        

>NTDB_id=151012 ADJ80_RS00025 WP_005704570.1 3521..4930(+) (pilB) [Aggregatibacter aphrophilus strain W10433]
ATGCAACATTCGATGGAACAACCTTATTCTATCTGTAGCCAACAGGGTGACATATTTACTATTACGCCCGAGTTATGGCA
ACAAAACCAACAGCAGCAAACCGTCCTTTTGCGTTATTTGGCGCTACCATTAAAAGAAGAACCACAAAAATTATGGCTGG
GATTGGATTCCTTAACCAACCTTGCCGCTTGTGAAGCGTTTTCCTTTTTAACAGGGAAAAATATCGAGCCGGTACTCATT
GAAAGTGCCGTGTTAAAAACGGCACTGCAAGATTTGGCGCCACATAGAGAAAAACTCGATGAAAATCAACCGCTCTTTTA
TTCCGTCACGCCTCAGGAGCAACAAGAACAATCCTCGGATGAGCCGACGATTCAATTGCTTAACCAAATTTTTGAAAACG
CCACAACAAAAAAAGCCTCCGATATTCATTTAGAACCGCAAGCAGATTTTTTACAAGTCCGTTTTCGTATTGACGGCGTA
TTACAAGTGCAAAACACGATTGCGCAGACACTCGCCAATCGACTGATCTCCCGTCTGAAATTACTGGCTAAATTAGACAT
CAGCGAAACCCGCCTGCCGCAAGACGGTCGATTTCAGTTTAAAACGACGTTTTCCGATATTTTGGATTTTCGCTTATCCA
CACTCGCTACGCATTTTGGCGAAAAAGCCGTATTGCGTTTACAACAAAACCGCCCCGTGCAACTGGCCTTTAGCGAACTG
GGCATGACGGAACAACAGCAACAAACATTTCGTCATGCGCTCAGCCAACCGCAAGGGTTAATTTTAGTCACCGGCCCCAC
GGGAAGCGGTAAAAGTATTTCTTTATATACGGCATTACAATGGCTCAATACCCCCGACAAGCATATTATGACGGCAGAAG
ATCCCATTGAAATTCAATTGCCGGGCATTATACAAAGCCAAGTGAATCCTCAAATCGGCTTGGATTTCAGTCGTTTGTTA
CGCACTTTTTTGCGTCAAGACCCCGACATTATTATGCTGGGTGAAATTCGTGACGAAGAAAGTGCCGCTATGGCCTTACG
GGCGGCACAAACCGGGCATCTGGTATTATCGACGTTACACACCAATGACGCCGCCTCAGCCATCTCACGCCTACAACAGC
TTGGCATTCAACAACACGAAATCGACAGCAGTTTATTATTGGTTATCGCCCAACGCTTGGTGCGTAAACGATGCCAAAAG
TGCGGTGAAAATTCCACGCGTTTTTGCGATTGTCATCAAGGTTACAAAGGTCGAATCGGTGTTTATCAATTCCTTCAGCC
TGATGTGTCACAAAACCAAAGTTATCAATTAGATTTTCAGAATTTATATGCCAGCGCATTAGAAAAAGTGAAGTCGCAAG
TGACGGATTTAGCAGAAGTGCAACGGGTATTGGGGCAACCGAATGCGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(129-396)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(20-97)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3S4QTD0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

73.42

97.868

0.719
  pilB Haemophilus influenzae Rd KW20

72.985

97.868

0.714
  pilB Glaesserella parasuis strain SC1401

58.568

98.294

0.576
  pilB Vibrio parahaemolyticus RIMD 2210633

42.38

100

0.433
  pilB Vibrio campbellii strain DS40M4

41.545

100

0.424
  pilB Acinetobacter baumannii D1279779

41.286

100

0.424
  pilB Vibrio cholerae strain A1552

41.25

100

0.422
  pilB Legionella pneumophila strain ERS1305867

38.323

100

0.409
  pilB Acinetobacter baylyi ADP1

38.431

100

0.407
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.471

90.618

0.367

Multiple sequence alignment