Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ADJ80_RS00020 Genome accession   NZ_CP012067
Coordinates   3047..3496 (+) Length   149 a.a.
NCBI ID   WP_050692438.1    Uniprot ID   -
Organism   Aggregatibacter aphrophilus strain W10433     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1..8496
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ADJ80_RS00010 (ADJ80_00010) nanQ 1425..1895 (+) 471 WP_050692437.1 N-acetylneuraminate anomerase -
  ADJ80_RS00015 (ADJ80_00015) ampD 2368..2922 (-) 555 WP_050694142.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ADJ80_RS00020 (ADJ80_00020) pilA 3047..3496 (+) 450 WP_050692438.1 pilin Machinery gene
  ADJ80_RS00025 (ADJ80_00025) pilB 3521..4930 (+) 1410 WP_005704570.1 GspE/PulE family protein Machinery gene
  ADJ80_RS00030 (ADJ80_00030) pilC 4923..6161 (+) 1239 WP_050692439.1 type II secretion system F family protein Machinery gene
  ADJ80_RS00035 (ADJ80_00035) - 6158..6850 (+) 693 WP_050692440.1 prepilin peptidase -
  ADJ80_RS00040 (ADJ80_00040) coaE 6903..7523 (+) 621 WP_050692441.1 dephospho-CoA kinase -
  ADJ80_RS00045 (ADJ80_00045) yacG 7516..7728 (+) 213 WP_005701268.1 DNA gyrase inhibitor YacG -
  ADJ80_RS00050 (ADJ80_00050) - 7728..7994 (+) 267 WP_005704566.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15275.69 Da        Isoelectric Point: 8.8087

>NTDB_id=151011 ADJ80_RS00020 WP_050692438.1 3047..3496(+) (pilA) [Aggregatibacter aphrophilus strain W10433]
MKIQSAFLSVKSVKKGFTLIELMIVIAIVAILATVAIPSYQNYTKKAAVSELLQASAPLRSEVELCVYNTGSVDNCTGGK
NGIQADVADATAKYVKSTAVKGGIITVVGKGSLDKVSYTLTAAGSATAGVTWTASCGTNADIFPAGFCS

Nucleotide


Download         Length: 450 bp        

>NTDB_id=151011 ADJ80_RS00020 WP_050692438.1 3047..3496(+) (pilA) [Aggregatibacter aphrophilus strain W10433]
ATGAAAATTCAGTCAGCATTTCTTTCTGTGAAATCCGTTAAAAAAGGATTTACATTAATTGAATTAATGATCGTGATTGC
GATTGTCGCCATTTTGGCTACTGTCGCGATTCCATCTTACCAAAATTACACCAAAAAAGCGGCAGTTTCCGAATTGTTAC
AAGCCTCTGCTCCACTTCGTTCCGAAGTCGAACTGTGCGTTTACAATACGGGCAGTGTTGATAACTGCACCGGCGGAAAA
AATGGCATTCAAGCCGATGTCGCCGACGCTACCGCAAAATATGTGAAATCAACCGCAGTGAAAGGCGGCATCATTACCGT
GGTCGGAAAAGGCAGCTTGGATAAAGTGTCTTACACACTAACCGCGGCAGGTTCTGCAACTGCCGGCGTCACATGGACAG
CCTCTTGCGGCACTAACGCCGACATCTTTCCGGCCGGCTTCTGTTCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(13-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

66.187

93.289

0.617
  pilA Haemophilus influenzae Rd KW20

64.539

94.631

0.611
  pilA Glaesserella parasuis strain SC1401

54.545

95.973

0.523

Multiple sequence alignment