Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACG33_RS12025 Genome accession   NZ_CP011971
Coordinates   2662491..2664233 (+) Length   580 a.a.
NCBI ID   WP_210399054.1    Uniprot ID   -
Organism   Steroidobacter denitrificans strain DSM 18526     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2657491..2669233
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACG33_RS11995 (ACG33_11995) - 2657780..2658064 (+) 285 WP_066921489.1 DUF1778 domain-containing protein -
  ACG33_RS12000 (ACG33_12000) - 2658065..2658589 (+) 525 WP_066921491.1 GNAT family N-acetyltransferase -
  ACG33_RS17100 - 2658715..2658912 (+) 198 Protein_2370 type II toxin-antitoxin system RelE/ParE family toxin -
  ACG33_RS12010 (ACG33_12010) - 2659237..2659809 (+) 573 WP_210399053.1 pilin -
  ACG33_RS12015 (ACG33_12015) - 2659906..2661102 (+) 1197 WP_066921495.1 isoprenylcysteine carboxylmethyltransferase family protein -
  ACG33_RS12020 (ACG33_12020) - 2661118..2662323 (+) 1206 WP_168160085.1 serine hydrolase -
  ACG33_RS12025 (ACG33_12025) pilB 2662491..2664233 (+) 1743 WP_210399054.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACG33_RS12030 (ACG33_12030) pilC 2664283..2665500 (+) 1218 WP_066921499.1 type II secretion system F family protein Machinery gene
  ACG33_RS12035 (ACG33_12035) - 2665629..2666603 (+) 975 WP_066921501.1 A24 family peptidase -
  ACG33_RS12040 (ACG33_12040) coaE 2666624..2667253 (+) 630 WP_066921503.1 dephospho-CoA kinase -
  ACG33_RS12045 (ACG33_12045) - 2667407..2669053 (+) 1647 WP_210399055.1 nodulation protein NfeD -

Sequence

Protein


Download         Length: 580 a.a.        Molecular weight: 63389.95 Da        Isoelectric Point: 5.3360

>NTDB_id=149776 ACG33_RS12025 WP_210399054.1 2662491..2664233(+) (pilB) [Steroidobacter denitrificans strain DSM 18526]
MTENVAPTLHAQRLALQGLPARLIQDGLLDERAMVQAMTEAKEAHTSLVGHLVSQGLADAREIAIAASQEFGVPLLDLDA
LQPDLETVRLVSDKLLQKHRVLPLIKRGKRLFVAVSDPTNLHALDEIRFQTGLSVEAIIVEEDKLQRVGSKAIEQVDAQM
PSLGEEDLDLENLDVTSGEDDGEREAVGRDDVEDAPIVRFVNKVMLDAIKKGASDIHFEPYEKTYRIRLRLDGHLKELAA
PPVQLANKISARIKVMSRLDIAERRIPQDGRIKMRISKNRAIDFRVSTCPTLFGEKIVARILDPSSAMLGIDALGYEPAQ
KDLYMKALAQPHGMILVTGPTGSGKTVSLYTGLNILNTEGTNISTAEDPAEINLPGVNQVNVNPKVGLTFASALRAFLRQ
DPDVIMVGEIRDLETAEIAIKAAQTGHLVLSTLHTNDAPKTLTRMVDMGVKPYAIATSVSLIIAQRLARRLCSHCKTPMD
MPPEALLKEGFREEDIAAGIRIYKPVGCSQCTDGYKGRTGLYQVMPVSEEIQKIILQDGNAVDIAAQSAAEGIWDLRRSG
LEKVKAGITSLDEVNQCTVE

Nucleotide


Download         Length: 1743 bp        

>NTDB_id=149776 ACG33_RS12025 WP_210399054.1 2662491..2664233(+) (pilB) [Steroidobacter denitrificans strain DSM 18526]
ATGACCGAAAACGTCGCTCCCACCCTCCATGCTCAGCGCCTGGCCCTGCAGGGACTGCCGGCGCGCCTGATCCAGGACGG
GCTTCTGGATGAGCGGGCGATGGTGCAGGCCATGACCGAGGCCAAGGAAGCCCATACCAGCCTGGTGGGGCACCTGGTCT
CCCAAGGTCTGGCCGACGCGCGCGAGATCGCCATCGCCGCCTCCCAGGAATTCGGTGTCCCGCTGCTGGATCTGGATGCG
CTCCAGCCGGATCTGGAAACCGTGCGGCTAGTGTCGGACAAGCTGCTGCAAAAGCACCGCGTCCTGCCTTTGATCAAGCG
CGGCAAGCGGCTGTTCGTCGCGGTTTCCGACCCGACCAACCTGCATGCGCTGGATGAAATCAGGTTCCAGACGGGCCTGT
CGGTGGAAGCCATCATCGTCGAGGAAGACAAGCTGCAGCGCGTGGGCAGCAAGGCGATCGAGCAGGTCGATGCCCAGATG
CCTTCCCTGGGCGAGGAAGACCTGGATCTCGAGAACCTGGACGTCACCAGCGGCGAAGACGACGGGGAGCGCGAGGCGGT
GGGCCGGGACGATGTCGAGGACGCCCCCATCGTCCGCTTCGTCAACAAGGTGATGCTGGATGCCATCAAGAAAGGTGCCT
CCGACATTCATTTCGAGCCCTACGAAAAGACCTACCGCATTCGCCTGCGCCTGGACGGTCACCTGAAGGAACTGGCCGCT
CCGCCGGTGCAACTGGCCAACAAGATTTCCGCCCGCATCAAGGTCATGTCGCGCCTGGACATCGCCGAGCGGCGCATCCC
TCAGGACGGCCGCATCAAGATGCGGATTTCCAAGAACCGGGCCATCGATTTTCGCGTCAGCACCTGCCCGACCCTGTTCG
GCGAAAAGATCGTCGCGCGTATCCTGGATCCCTCCAGCGCCATGCTGGGCATCGATGCGCTGGGCTATGAGCCGGCGCAG
AAAGACCTGTACATGAAGGCACTGGCCCAACCCCACGGCATGATCCTGGTCACCGGGCCGACGGGCAGCGGCAAGACCGT
GTCGCTGTACACCGGACTCAACATCCTCAACACGGAAGGCACCAACATTTCCACCGCGGAGGATCCGGCGGAAATCAATC
TTCCCGGGGTCAACCAGGTCAACGTGAATCCGAAGGTCGGCCTGACGTTCGCCTCGGCCCTGCGCGCCTTCCTGCGCCAG
GATCCGGATGTGATCATGGTCGGTGAGATTCGCGACCTGGAAACCGCCGAGATCGCCATTAAGGCGGCGCAGACCGGTCA
CCTGGTCCTCTCGACCTTGCACACCAACGATGCGCCCAAGACCTTGACCCGCATGGTCGACATGGGGGTCAAGCCCTACG
CCATCGCCACCTCCGTCTCCCTGATCATCGCCCAGCGCCTGGCACGCCGGCTTTGCAGTCACTGCAAGACGCCGATGGAC
ATGCCGCCGGAGGCGTTGCTGAAGGAAGGCTTCAGAGAGGAGGACATCGCCGCCGGTATCAGGATCTACAAGCCGGTCGG
CTGCAGCCAATGCACGGACGGCTACAAGGGACGCACCGGCCTGTACCAGGTCATGCCGGTTTCCGAGGAGATCCAGAAGA
TCATCCTCCAGGACGGCAATGCGGTCGACATTGCCGCGCAAAGCGCCGCGGAGGGCATCTGGGATCTGCGGCGTTCGGGA
CTGGAAAAGGTCAAGGCCGGCATCACCAGCCTGGATGAAGTCAATCAGTGCACGGTCGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(203-470)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.923

97.931

0.567
  pilB Acinetobacter baylyi ADP1

57.774

97.586

0.564
  pilB Legionella pneumophila strain ERS1305867

53.484

98.966

0.529
  pilB Vibrio cholerae strain A1552

50.615

98.103

0.497
  pilB Vibrio campbellii strain DS40M4

49.296

97.931

0.483
  pilB Vibrio parahaemolyticus RIMD 2210633

49.027

97.414

0.478
  pilF Neisseria gonorrhoeae MS11

51.311

92.069

0.472
  pilB/pilB1 Synechocystis sp. PCC 6803

36.482

100

0.386
  pilF Thermus thermophilus HB27

37.367

96.897

0.362

Multiple sequence alignment