Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACU14_RS06585 Genome accession   NZ_CP011960
Coordinates   1483722..1484852 (+) Length   376 a.a.
NCBI ID   WP_014502536.1    Uniprot ID   G7TJ40
Organism   Xanthomonas oryzae pv. oryzicola strain L8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1478722..1489852
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACU14_RS06560 (ACU14_06565) - 1478878..1479519 (-) 642 WP_047339651.1 M23 family metallopeptidase -
  ACU14_RS06565 (ACU14_06570) - 1479626..1480051 (-) 426 WP_014502532.1 HU family DNA-binding protein -
  ACU14_RS06570 (ACU14_06575) proC 1480609..1481469 (-) 861 WP_048483156.1 pyrroline-5-carboxylate reductase -
  ACU14_RS06575 (ACU14_06580) - 1481513..1482205 (-) 693 WP_048483157.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACU14_RS06580 (ACU14_06585) pilT 1482571..1483608 (+) 1038 WP_014502535.1 type IV pilus twitching motility protein PilT Machinery gene
  ACU14_RS06585 (ACU14_06590) pilU 1483722..1484852 (+) 1131 WP_014502536.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACU14_RS06590 (ACU14_06595) - 1485124..1485798 (+) 675 WP_024711780.1 YitT family protein -
  ACU14_RS06595 (ACU14_06600) - 1486025..1486858 (-) 834 WP_014502538.1 DUF72 domain-containing protein -
  ACU14_RS06600 (ACU14_06605) - 1486895..1487467 (-) 573 WP_014502539.1 DNA-3-methyladenine glycosylase I -
  ACU14_RS25095 - 1487464..1488662 (-) 1199 Protein_1299 hypothetical protein -
  ACU14_RS06615 (ACU14_06620) - 1488738..1489304 (+) 567 WP_024711782.1 YqgE/AlgH family protein -
  ACU14_RS06620 (ACU14_06625) ruvX 1489297..1489764 (+) 468 WP_014502544.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41940.17 Da        Isoelectric Point: 6.8287

>NTDB_id=149667 ACU14_RS06585 WP_014502536.1 1483722..1484852(+) (pilU) [Xanthomonas oryzae pv. oryzicola strain L8]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVNGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVDELSLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGHRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWESALKNTLRQAPDVIMIGEVRAREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRSRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLSQGMDGVEISEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=149667 ACU14_RS06585 WP_014502536.1 1483722..1484852(+) (pilU) [Xanthomonas oryzae pv. oryzicola strain L8]
ATGAGCACCATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCATCAGAAGGCGTCGGACCTGTTCATCACCTCAGGCAT
GCCACCGGCGATCAAGGTGAATGGCAAGATCAGCCCGATTACCCAGACGCCGCTGACTGCGCAGCAGAGCCGCGATCTGG
TGTTGAACGTGATGACGCCCTCGCAGCGCGAGGAATTCGAAAAGACCCACGAGTGCAACTTCGCCATCGGCGTTTCAGGG
GTGGGGCGTTTTCGTGTGAGCTGTTTCTACCAGCGTAATCAGGTCGGTATGGTGCTGCGCCGGATCGAAACGCGCATCCC
CACCGTGGACGAGCTGAGCCTGCCTCCGGTGATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGTACCGGTAAGTCGACCTCGCTGGCGGCGATGATCGGCCACCGCAACCAGAATTCCACCGGCCACATCATCACC
ATCGAAGACCCGATCGAATTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAGGTCGGCATCGATACCGACAG
CTGGGAAAGCGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGTGCCCGCGAGGGCA
TGGACCACGCGATCGCCTTCGCCGAAACCGGCCACCTGGTGCTGTGCACGCTGCACGCCAACAACGCCAATCAGGCGATG
GACCGCATCATCAACTTCTTCCCCGAAGACCGCCGCAATCAGCTGCTGATGGACCTGTCGCTCAATCTCAAGGGCGTGGT
GGCGCAGCAGCTGATTCCGACGCCGGATGGCCGCAGCCGCCGCGTGGCGATGGAAATCATGTTGGGTACGCCGCTGGTGC
AGGACTACATCCGCGACGGCGAAATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACCTGGGCATGCGCACCTTC
GACCAGAGCCTGTTCGAGCTCTACCAGGCTGGCGAAATCAGCTACGAAGATGCATTGCGCTACGCCGACTCGCAGAACGA
AGTGCGCTTGCGCATCAAGCTCTCGCAGGGGGGCGATGCCAAGACCTTGTCGCAGGGCATGGATGGTGTGGAGATTTCCG
AAGTTCGATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(108-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G7TJ40

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.485

97.606

0.649
  pilU Acinetobacter baylyi ADP1

64.986

94.947

0.617
  pilU Vibrio cholerae strain A1552

52.809

94.681

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.017

94.947

0.399
  pilT Acinetobacter nosocomialis M2

39.535

91.489

0.362
  pilT Acinetobacter baumannii D1279779

39.535

91.489

0.362
  pilT Acinetobacter baumannii strain A118

39.535

91.489

0.362

Multiple sequence alignment