Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACP86_RS02405 Genome accession   NZ_CP011929
Coordinates   511718..513436 (+) Length   572 a.a.
NCBI ID   WP_053112652.1    Uniprot ID   A0A0K1U5S5
Organism   Marinobacter sp. CP1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 506718..518436
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACP86_RS02385 (ACP86_02380) - 507324..508685 (+) 1362 WP_053112648.1 MBL fold metallo-hydrolase -
  ACP86_RS02390 (ACP86_02385) - 508682..509125 (-) 444 WP_053112649.1 pilin -
  ACP86_RS02395 (ACP86_02390) - 509185..510714 (-) 1530 WP_053112650.1 O-antigen ligase family protein -
  ACP86_RS02400 (ACP86_02395) - 510833..511330 (-) 498 WP_053112651.1 pilin -
  ACP86_RS02405 (ACP86_02400) pilB 511718..513436 (+) 1719 WP_053112652.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACP86_RS02410 (ACP86_02405) pilC 513439..514659 (+) 1221 WP_053112653.1 type II secretion system F family protein Machinery gene
  ACP86_RS02415 (ACP86_02410) pilD 514726..515601 (+) 876 WP_053112654.1 prepilin peptidase Machinery gene
  ACP86_RS02420 (ACP86_02415) coaE 515605..516204 (+) 600 WP_053112655.1 dephospho-CoA kinase -
  ACP86_RS02425 (ACP86_02420) - 516197..517291 (+) 1095 WP_053112656.1 hypothetical protein -
  ACP86_RS02430 (ACP86_02425) tsaA 517291..518034 (+) 744 WP_053112657.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62888.85 Da        Isoelectric Point: 4.9014

>NTDB_id=149342 ACP86_RS02405 WP_053112652.1 511718..513436(+) (pilB) [Marinobacter sp. CP1]
MSTNKTNVTLTGLARRFVDDGLLDESTAKDAFLQASQNRIPLITYLTQNNLADSSKLAFSAAMEFGVSVLDLSAFMPEMM
PDKVVDEKLIRKHNALPLYKRGNRLFIAVSDPTNIQALDEIKFNTGLSTDGILVDDAKLREAIDKYLESHDTTMGDLDDA
DLEGVETEGGEQEDDDGAVSASDVDDAPIVKYVNKMLLDAIRGGASDIHFEPYEKTYRVRYRTDGILKEVSRPSIKLAPK
ISARVKIMAQLDISERRVPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVLRILDPSQAKLGIDVLGYEEDQKKLYMDSL
AQPQGMILVTGPTGSGKTVSLYTGLNILNTPGINISTAEDPAEINLEGINQVNVNTRVGLGFAEALRAFLRQDPDVIMVG
EIRDLETANIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPSFNIATSVSLIIAQRLGRRLCNSCKQAFDVPKDVLLE
EGFTQEQIDTGFTLYRPKGCDKCNGGYKGRVGIYEVVKITDELANMIMEEASSIKIAKQAQADGFRNLRQSALMKVIEGV
TSLEEANRVTKD

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=149342 ACP86_RS02405 WP_053112652.1 511718..513436(+) (pilB) [Marinobacter sp. CP1]
ATGTCTACCAATAAAACCAACGTGACTCTAACCGGGCTGGCGAGGCGCTTCGTCGATGATGGGCTTCTTGATGAATCCAC
CGCTAAGGACGCATTCCTCCAGGCTTCGCAAAATCGCATTCCACTGATTACTTACCTGACCCAGAACAACCTGGCGGATA
GCTCGAAGCTTGCTTTTTCCGCTGCCATGGAGTTCGGGGTTTCAGTACTGGATCTCTCGGCATTCATGCCGGAAATGATG
CCGGACAAGGTGGTAGATGAGAAACTGATCCGCAAACACAACGCGCTTCCCCTCTATAAACGCGGGAATCGTTTGTTTAT
TGCGGTTTCGGACCCGACCAATATCCAGGCACTTGATGAGATCAAGTTCAATACCGGCCTGAGCACAGATGGCATTCTGG
TAGATGACGCAAAACTGCGAGAAGCAATCGACAAGTATCTGGAATCCCATGATACGACGATGGGAGATCTTGACGATGCC
GACCTCGAGGGCGTCGAGACGGAGGGGGGCGAACAGGAAGATGACGACGGCGCTGTATCTGCCAGTGATGTCGATGACGC
GCCGATCGTCAAATACGTCAATAAGATGCTGCTCGACGCCATTCGCGGTGGCGCGTCAGACATCCACTTTGAGCCGTACG
AAAAGACCTACCGCGTTCGCTACCGTACGGATGGCATTCTCAAGGAAGTCTCCCGCCCCTCTATCAAGCTGGCACCGAAA
ATCTCCGCCCGCGTAAAGATCATGGCTCAACTTGATATTTCAGAGAGACGGGTGCCTCAGGACGGTCGTATCAAGATGAA
GCTGTCCAAAACCAAGGCGATCGATTTCCGGGTAAACACGCTCCCTACTCTCTGGGGCGAGAAGATCGTGCTTCGGATTC
TGGACCCGAGTCAGGCAAAACTGGGCATCGACGTGCTCGGGTACGAGGAAGACCAGAAGAAGCTGTATATGGACTCCCTT
GCTCAACCCCAGGGCATGATTCTGGTAACGGGCCCAACTGGTTCTGGTAAGACCGTTTCCCTGTACACCGGCCTGAACAT
CCTGAACACCCCCGGAATCAATATTTCCACCGCGGAAGACCCGGCGGAAATCAATTTGGAGGGCATCAACCAGGTAAATG
TGAACACCAGGGTCGGGCTCGGTTTCGCGGAGGCCCTGAGAGCCTTCCTGCGTCAAGACCCCGACGTGATCATGGTGGGT
GAGATTCGGGATCTTGAAACGGCCAACATTGCGATCAAGGCTGCGCAGACCGGCCACCTGGTTCTTTCAACCCTGCATAC
CAACAGTGCGGCTGAAACGCTCACCCGGATGATGAATATGGGCGTGCCCTCGTTCAACATCGCTACCTCAGTAAGCCTGA
TTATTGCCCAGCGTCTTGGCCGGCGACTCTGCAATTCCTGTAAGCAAGCCTTCGACGTTCCCAAGGACGTACTTTTGGAG
GAGGGGTTCACACAGGAACAAATTGATACAGGCTTCACGTTGTACCGCCCCAAGGGCTGTGATAAATGCAATGGAGGCTA
TAAAGGCCGCGTTGGTATTTACGAGGTGGTCAAGATTACCGACGAGCTGGCGAACATGATTATGGAAGAAGCCAGCTCCA
TCAAAATTGCAAAGCAGGCTCAGGCTGACGGCTTCCGCAACTTGCGACAGTCCGCACTCATGAAAGTGATCGAGGGCGTG
ACAAGCCTTGAGGAAGCCAATCGCGTGACGAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(195-462)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0K1U5S5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.846

100

0.573
  pilB Acinetobacter baylyi ADP1

56.37

100

0.565
  pilB Legionella pneumophila strain ERS1305867

54.145

99.126

0.537
  pilB Vibrio parahaemolyticus RIMD 2210633

51.532

97.028

0.5
  pilB Vibrio cholerae strain A1552

54.913

90.734

0.498
  pilB Vibrio campbellii strain DS40M4

50.088

98.776

0.495
  pilF Neisseria gonorrhoeae MS11

47.09

99.126

0.467
  pilB/pilB1 Synechocystis sp. PCC 6803

41.538

90.909

0.378
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.469

92.133

0.364

Multiple sequence alignment